Preparation and partial characterisation of a multilamellar body fraction from Schistosoma mansoni

McDiarmid, Shona S.; Podesta, Ron B.; Rahman, S M Khaledur

doi:10.1016/0166-6851(82)90044-5

Cited by 24 publications

(5 citation statements)

References 31 publications

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“…They suggested that they add to and replace the plasma membrane. Recent work on these bodies in 8. mansoni by McDiarmid, Podesta, & Rahman (1982) suggests that their function may be more complex since these workers detected high activity levels for phosphatidic acid phosphatase. This would suggest a more active role for schistosome spherical bodies than that of a reservoir of pre-formed membrane precursors.…”

Section: Discussionmentioning

confidence: 99%

The ultrastructure of the tegument of adult Schistosoma haematobium

1984

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The ultrastructure of the tegument of Schistosoma haematobium was examined using scanning and transmission electron microscopy. The surface of the male worm is characterized by numerous raised tubercles bearing apically directed spines. The female in contrast to the male is cylindrical and relatively smooth. Details of oral and ventral suckers are given. The use of uranyl acetate as a tertiary fixative and en bloc stain has revealed the heptalaminate nature of the outer membrane. Tegumental mitochondria are shown to be morphologically more complex than those of S. mansoni. Spherical and elliptical inclusion bodies are also described. The ultrastructure of the oesophageal tegument of S. haematobium is described for the first time and corresponds with earlier observations of S. mansoni.

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Section: Discussionmentioning

confidence: 99%

The ultrastructure of the tegument of adult Schistosoma haematobium

1984

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“…At least 25% of the total phospholipids of multilamellar bodies of S. mansoni are acidic ones: mainly phosphatidylserine (PS) (15%), phosphatidylglycerol (PG) (8%), phosphatidylinositol (5%), and phosphatidic acid. Neutral phospholipids consisting of phosphatidylethanolamine (PE) (25%) and phosphatidylcholine (PC) (28%) are the main fraction (McDiarmid et al 1982). Schepers et al (1988) could show that PZQ is able to destabilize liposomes of synthetic dipalmitoylphosphatidylcholine (DPPC) liposomes by insertion into the bilayer.…”

Section: Phospholipid Composition Of Schistosome Tegumentmentioning

confidence: 99%

Activation of transient receptor potential channel Sm.(Schistosoma mansoni)TRPMPZQ by PZQ, enhanced Ca++ influx, spastic paralysis, and tegumental disrupture—the deadly cascade in parasitic schistosomes, other trematodes, and cestodes

Harder

2020

Parasitol Res

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After almost 50 years of praziquantel (PZQ) research, Park and Marchant (Trends Parasitol 36:182–194, 2020 ) described the Ca ++ -permeable transient receptor potential (TRP) channel Sm.TRPM PZQ in Schistosoma mansoni as target of PZQ. Here we describe the deadly cascade in schistosomes which is induced by the (R)-PZQ enantiomer that includes contemporaneous stereoselective activation of Sm.TRPM PZQ -mediated Ca ++ influx, disturbed Ca ++ homeostasis, Ca ++ -dependent spastic paralysis, and Ca ++ - and PZQ-dependent disruption of parasitic teguments. Under normal conditions, there is a reversible balance between bilayer, isotropic, and HII phases in biological membranes (Jouhet 2013 ). In vitro, we could observe an irreversible but not stereoselective transition to the HII phase in liposomes consisting of phosphatidylethanolamine (PE) and phosphatidylserine (PS), two naturally occurring phospholipids in schistosomes, by the concerted action of Ca ++ and PZQ (Harder 2013 ). HII structures are a prerequisite for induction of fusion processes (Jouhet 2013 ), which, indeed, become visible as blebs, vacuolation processes, and large balloon-like surface exudates in a large variety of PZQ-sensitive parasitic flukes and cestodes after PZQ treatment. These tegument damages are irreversible. As homologs of Sm.TRPM PZQ are also present in the other trematodes S. japonicum , S. haematobium , or Clonorchis sinensis and cestodes Taenia solium , Echinococcus multilocularis , or Hymenolepis microstoma (Park and Marchant, Trends Parasitol 36:182–194, 2020 ), it is suggested that a similar deadly cascade will be operating generally in PZQ-sensitive parasites.

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“…The best approximation is derived from the composition of the multilaminate vesicles, which are contained in the tegument and which are thought to be incorporated into the surface of schistosomes; at least 25 % of the total phospholipids of multilamellar bodies of S. mansoni are acidic ones: mainly phosphatidylserine (PS) (15 %), phosphatidylglycerol (PG) (8 %), phosphatidylinositol (5 %), and phosphatidic acid. Neutral phospholipids consisting of phosphatidylethanolamine (PE) (25 %) and phosphatidylcholine (PC) (28 %) are the main fraction (McDiarmid et al 1982). The initial studies were conducted using differential thermal analysis, a method which allows the measurement of stability of phospholipid membranes (e.g., acidic PG) in the presence of different drugs and ions.…”

Section: Effects Of Praziquantel On Model Membranesmentioning

confidence: 99%

Praziquantel

Harder

2013

Treatment of Human Parasitosis in Traditional Chinese Medicine

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Praziquantel is the most common antiparasitic drug against all types of trematodes and cestodes. Although discovered in Germany at Bayer Company, it came first on the market in China due to the high pressure of severe diseases such as schistosomiasis in Asia. This very efficient and safe compound is today the gold standard in the treatment of human and animal infections due to Platyhelminthes. Thus all plant-derived products-old and new ones-have to be compared with its high standards.

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Preparation and partial characterisation of a multilamellar body fraction from Schistosoma mansoni

Cited by 24 publications

References 31 publications

The ultrastructure of the tegument of adult Schistosoma haematobium

The ultrastructure of the tegument of adult Schistosoma haematobium

Activation of transient receptor potential channel Sm.(Schistosoma mansoni)TRPMPZQ by PZQ, enhanced Ca++ influx, spastic paralysis, and tegumental disrupture—the deadly cascade in parasitic schistosomes, other trematodes, and cestodes

Praziquantel

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