Preparation and mechanical optimization of a two‐layer silk/magnesium wires braided porous artificial nerve guidance conduit

Zhao, Jingyuan; Zhang, Shujun; Duan, Lirong; Yao, Ruotong; Yan, Yonggang; Wang, Jing; Zheng, Zhaozhu; Wang, Xiaoqin; Li, Gang

doi:10.1002/jbm.a.37426

Cited by 5 publications

(2 citation statements)

References 38 publications

(62 reference statements)

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“…( m , n ) multichannel conduits [ 101 , 108 ]. ( o ) NGC containing multiple biomimetic features [ 112 ].…”

Section: Figurementioning

confidence: 99%

Advances in Biomimetic Nerve Guidance Conduits for Peripheral Nerve Regeneration

Mankavi,

Ibrahim,

Wang

2023

Nanomaterials

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Injuries to the peripheral nervous system are a common clinical issue, causing dysfunctions of the motor and sensory systems. Surgical interventions such as nerve autografting are necessary to repair damaged nerves. Even with autografting, i.e., the gold standard, malfunctioning and mismatches between the injured and donor nerves often lead to unwanted failure. Thus, there is an urgent need for a new intervention in clinical practice to achieve full functional recovery. Nerve guidance conduits (NGCs), providing physicochemical cues to guide neural regeneration, have great potential for the clinical regeneration of peripheral nerves. Typically, NGCs are tubular structures with various configurations to create a microenvironment that induces the oriented and accelerated growth of axons and promotes neuron cell migration and tissue maturation within the injured tissue. Once the native neural environment is better understood, ideal NGCs should maximally recapitulate those key physiological attributes for better neural regeneration. Indeed, NGC design has evolved from solely physical guidance to biochemical stimulation. NGC fabrication requires fundamental considerations of distinct nerve structures, the associated extracellular compositions (extracellular matrices, growth factors, and cytokines), cellular components, and advanced fabrication technologies that can mimic the structure and morphology of native extracellular matrices. Thus, this review mainly summarizes the recent advances in the state-of-the-art NGCs in terms of biomaterial innovations, structural design, and advanced fabrication technologies and provides an in-depth discussion of cellular responses (adhesion, spreading, and alignment) to such biomimetic cues for neural regeneration and repair.

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“…( m , n ) multichannel conduits [ 101 , 108 ]. ( o ) NGC containing multiple biomimetic features [ 112 ].…”

Section: Figurementioning

confidence: 99%

Advances in Biomimetic Nerve Guidance Conduits for Peripheral Nerve Regeneration

Mankavi,

Ibrahim,

Wang

2023

Nanomaterials

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“…In particular, Li et al and Pixley et al have used Mg metal wires in their studies, with positive effects on nerve repair [15,[20][21][22]. However, while many of the in vivo studies have targeted the effects on axons and inflammation, observing the nerve as a whole, few studies have specifically studied the effects on SCs [14,23], and the in vitro studies focused mainly on cytocompatibility [24,25] or did not consider the injury microenvironment [17,19]. In summary, there is a need for improved understanding of SC responses to Mg material degradation.…”

Section: Introductionmentioning

confidence: 99%

Influence of Magnesium Degradation on Schwannoma Cell Responses to Nerve Injury Using an In Vitro Injury Model

Bhat,

Hanke,

Helmholz

et al. 2024

JFB

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Nerve guidance conduits for peripheral nerve injuries can be improved using bioactive materials such as magnesium (Mg) and its alloys, which could provide both structural and trophic support. Therefore, we investigated whether exposure to Mg and Mg-1.6wt%Li thin films (Mg/Mg‑1.6Li) would alter acute Schwann cell responses to injury. Using the RT4-D6P2T Schwannoma cell line (SCs), we tested extracts from freeze-killed cells (FKC) and nerves (FKN) as in vitro injury stimulants. Both FKC and FKN induced SC release of the macrophage chemoattractant protein 1 (MCP-1), a marker of the repair SC phenotype after injury. Next, FKC-stimulated cells exposed to Mg/Mg‑1.6Li reduced MCP-1 release by 30%, suggesting that these materials could have anti-inflammatory effects. Exposing FKC-treated cells to Mg/Mg‑1.6Li reduced the gene expression of the nerve growth factor (NGF), glial cell line-derived neurotrophic factor (GDNF), and myelin protein zero (MPZ), but not the p75 neurotrophin receptor. In the absence of FKC, Mg/Mg‑1.6Li treatment increased the expression of NGF, p75, and MPZ, which can be beneficial to nerve regeneration. Thus, the presence of Mg can differentially alter SCs, depending on the microenvironment. These results demonstrate the applicability of this in vitro nerve injury model, and that Mg has wide-ranging effects on the repair SC phenotype.

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Fabrication of Silk Fibroin‐Derived Fibrous Scaffold for Biomedical Frontiers

Rahman,

Dip,

Nur

et al. 2024

Macro Materials & Eng

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Silk fibroin (SF), a natural protein derived from silkworms, has emerged as a promising biomaterial due to its biocompatibility, biodegradability, degradation rate, and tunable mechanical properties. This review delves into the intrinsic attributes of SF that make it an attractive candidate for scaffold development in tissue engineering and regenerative medicine. The distinctiveness of this comprehensive review resides in its detailed exploration of recent advancements in the fabrication techniques of SF‐based fibrous scaffolds, namely electrospinning, freeze‐drying, and 3D printing. An in‐depth analysis of these fabrication techniques is conducted to illustrate their versatility in customizing essential scaffold characteristics, such as porosity, fiber diameter, and mechanical strength. The article meticulously discusses process parameters, advantages, and challenges of each fabrication technique, highlighting the innovative advancements made in the respective field. Furthermore, the review goes beyond fabrication techniques to provide an overview of the latest biomedical applications and research endeavors utilizing SF‐derived scaffolds. From nerve regeneration and wound healing to drug delivery, bone regeneration, and vascular tissue engineering, the diverse applications underscore the versatility of SF in adopting various biomedical challenges. Finally, the article emphasizes the need for standardized characterization techniques, scalable manufacturing processes, and long‐term in vivo studies.

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Preparation and mechanical optimization of a two‐layer silk/magnesium wires braided porous artificial nerve guidance conduit

Cited by 5 publications

References 38 publications

Advances in Biomimetic Nerve Guidance Conduits for Peripheral Nerve Regeneration

Advances in Biomimetic Nerve Guidance Conduits for Peripheral Nerve Regeneration

Influence of Magnesium Degradation on Schwannoma Cell Responses to Nerve Injury Using an In Vitro Injury Model

Fabrication of Silk Fibroin‐Derived Fibrous Scaffold for Biomedical Frontiers

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