Preparation and investigation on properties of lysozyme chemically bonded to single-walled carbon nanotubes

Borzooeian, Zahra; Safavi, Afsaneh; Sheikhi, Mohammad Hossein; Aminlari, Mahmood; Doroodmand, Mohammad Mahdi

doi:10.1080/17458081003699122

Cited by 10 publications

(13 citation statements)

References 30 publications

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“…Highly puried activated SWCNTs were synthesized by the plasma-enhanced chemical vapour deposition (PECVD) process. 44 The SWCNTs' outer diameters were 1-3 nm.…”

Section: Methodsmentioning

confidence: 99%

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Activity and stability analysis of covalent conjugated lysozyme-single walled carbon nanotubes: potential biomedical and industrial applications

et al. 2017

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“…Highly puried activated SWCNTs were synthesized by the plasma-enhanced chemical vapour deposition (PECVD) process. 44 The SWCNTs' outer diameters were 1-3 nm.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we evaluated the conjugation of chicken egg white lysozyme (EWL) with single-walled carbon nanotubes (SWCNTs) structurally. 44 Lysozymes are a family of enzymes with antimicrobial activity. They are present in saliva, tears, egg white, and many animal uids.…”

Section: Introductionmentioning

confidence: 99%

Activity and stability analysis of covalent conjugated lysozyme-single walled carbon nanotubes: potential biomedical and industrial applications

et al. 2017

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“…Carbodiimide method 19,21,25 was used for bio-conjugation of lysozyme onto carboxylated carbon nanotubes surface. The results from SEM micrographs ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Details of covalent conjugation of lysozyme (lyophilized chicken egg white lysozyme, EC 3.2.1.17, Inovatech, Inc. Abbotsford, BC, Canada) to the functionalized single-walled carbon nanotubes (SWCNT-COOH) with outer diameter of 1-2 nm (MKnano, Canada) were given in our previous work. 19,20 Conjugation was achieved using carbodiimide method. 21 One mg of CNTs was dispersed in one mL of MES buffer, 50 mM, pH 6.2, and was added to an equal volume of 400 mM NHS in MES buffer.…”

Section: Conjugation Of Lysozyme Onto Swcntsmentioning

confidence: 99%

A high precision length-based carbon nanotube ladder

et al. 2018

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“…The antibacterial enzyme lysozyme (LSZ) has garnered significant attention as an intriguing SWNT dispersant. It has been considered as a model system for understanding fundamental nanotube-protein interactions [14][15][16][17][18][19][20][21] as well as the effects of nanotubes on protein activity [18,[22][23][24][25], gelation, [26], crystallization [27], and separation [28,29]. In addition, high strength, antimicrobial fibers [11,30], and films [31] have been produced from LSZ/SWNT dispersions.…”

Section: Introductionmentioning

confidence: 99%

Concentration of lysozyme/single-walled carbon nanotube dispersions

Horn

Davis

2016

Colloids and Surfaces B: Biointerfaces

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The dispersion of single-walled carbon nanotubes (SWNT) in aqueous solutions of biological materials enables the production of bulk films and fibers that combine natural biological activity with SWNT's intrinsic mechanical, thermal, and electrical properties. In this work, we report the rheology and phase behavior of concentrated lysozyme (LSZ)/SWNT dispersions. Even at low concentration, the LSZ's globular structure causes a deviation from the rheological behavior expected of rigid rods such as SWNT. With increasing concentration, stabilized SWNT typically form lyotropic liquid crystalline phases. However, in this case, the LSZ results in depletion attraction and the formation of large dense SWNT aggregates surrounded by a LSZ network. At intermediate concentrations, the microstructure and rheological properties are a complex function of the initial dispersion state, the absolute concentrations, and the LSZ to SWNT ratio. The rheological effects of concentrating mixtures comprised of aggregates, a range of bundle sizes, and individual SWNT were compared to the effects of concentrating supernatants comprised solely of individual SWNT and small bundles. In general, lysozyme concentration has the greatest impact on dispersion viscoelasticity. However, the inherent viscosity was a function of SWNT concentration; data from both initial mixtures and supernatants spanning two orders of magnitude in concentration could be collapsed onto a single master curve. This work provides a foundation for exploring the behavior of other globular protein-SWNT dispersions.

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Preparation and investigation on properties of lysozyme chemically bonded to single-walled carbon nanotubes

Cited by 10 publications

References 30 publications

Activity and stability analysis of covalent conjugated lysozyme-single walled carbon nanotubes: potential biomedical and industrial applications

Activity and stability analysis of covalent conjugated lysozyme-single walled carbon nanotubes: potential biomedical and industrial applications

A high precision length-based carbon nanotube ladder

Concentration of lysozyme/single-walled carbon nanotube dispersions

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