Preparation and Infusion of Cryoprecipitate from Exercised Donors

Gastel, C. van; Sixma, JJ; Borst-Eilers, E; Leautaud, Marlène; Moes, Mieke; Plas, Pascal; Bouma, Bonno N.; Sybesma, J. P. H. B.

doi:10.1111/j.1365-2141.1973.tb01759.x

Cited by 14 publications

(4 citation statements)

References 11 publications

(4 reference statements)

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“…In trials employing similar intensity, duration and type of exercise (bicycle ergometry) a20 to 60Vo increase in vWF (23,24) and an about 40 to 507o increase in FVIII (9, 25) were observed similar to our placebo period. In agreement with our results Speiser et al (25) also observed the maximal increase in vWF not immediately after exercise but 60 min later (Fig.…”

Section: Discussionsupporting

confidence: 78%

Partial Blockade of Nitric Oxide Synthase Blunts the Exercise-induced Increase of von Willebrand Factor Antigen and of Factor VIII in Man

et al. 1997

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SummaryBackground: Until now the effects of β-adrenergic agonists have largely been ascribed to their ability to induce intracellular formation of cyclic adenosine monophosphate. Recently evidence has been accumulating that at least some β1 and β2-adrenoceptor effects may be mediated by nitric oxide (NO). Based on these studies, we hypothesized that the β-adrenoceptor mediated increase of von Willebrand factor and factor VIII-activity (FVIII:C) in plasma during exercise, is caused by an NO-dependent mechanism. Methods: Thirteen young healthy subjects finished an exhaustive bicycle exercise protocol while they were infused placebo or the NO-synthase inhibitor N-monomethyl-L-arginine (L-NMMA) on two separate days in a randomized, double blind cross-over design. Findings: During exercise systemic haemo-dynamic changes were parallel in both treatment periods, but L-NMMA caused a partial inhibition of NO-synthase as evidenced by a 30% decrease in exhaled NO. The workload capacities were not different during L-NMMA or placebo infusion. However, under placebo treatment exercise increased vWF-Ag by a maximum of 61% (CI: 43-84; p = 0.002) and FVIII:C by 44% (CI: 31-59; p = 0.001), which was significantly attenuated when subjects were treated with L-NMMA (p <0.05): under L-NMMA treatment vWF-Ag increased by only 25% (CI: 5-51; p = 0.001) and FVIII:C by 12% (CI: 6-39; p = 0.001). Interpretation: Partial blockade of NO-synthase with L-NMMA blunts the exercise-induced increase in vWF-Ag and FVIII:C. Our trial points to a role of endogenous NO-generation in the β2-adrenergic increase in vWF/FVIII. Thus, we propose that physiologic processes which are induced by systemic β2-adrenoceptor stimulation may at least partly be mediated by NO.

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Section: Discussionsupporting

confidence: 78%

Partial Blockade of Nitric Oxide Synthase Blunts the Exercise-induced Increase of von Willebrand Factor Antigen and of Factor VIII in Man

et al. 1997

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“…In this way the biphasic nature of the disappearance curve was discovered. The early data showed some discordance, but more recent studies have shown that the t1/2s are in the range of about [12][13][14][15][16][17][18] h. The introduction of the quantitative assay for VIIIR:Ag using heterologous antisera (2) allowed the analysis of the disappearance of Factor VIII on the basis of VIIIR:Ag. It was then reported that this VIIIR:Ag decreased more slowly than VIII:C after transfusion of normal cryoprecipitate into hemophiliacs (15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

“…Studies concerning the survival of Factor VIII in humans have been based initially on the disappearance of VIII:C administered to classic hemophilia patients (12)(13)(14). In this way the biphasic nature of the disappearance curve was discovered.…”

Section: Introductionmentioning

confidence: 99%

Survival of 125iodine-labeled Factor VIII in normals and patients with classic hemophilia. Observations on the heterogeneity of human Factor VIII.

Over¹,

Sixma²,

Bruïne³

et al. 1978

J. Clin. Invest.

100

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“…As these data correspond to those known from the literature [4, the optimalized procedure results in a higher yield of stable factor VIII, which is suitable for clinical use. The extra amount of factor VIII obtained by this method is not a labile form of this factor [7].…”

Section: Improvements Of the Final Product By Changes Irz The Preparamentioning

confidence: 86%

Contributions to the Optimal Use of Human Blood

et al. 1976

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The influence of different variables on the yield of factor VIII in cryoprecipitate as prepared in the Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, was studied. The following conclusions may be drawn: (1) In case blood should be stored, the use of the anticoagulant solution acid citrate dextrose (ACD) is preferable to citrate phosphate dextrose (CPD) or trisodium citrate (TSC). (2) The temperature of stored whole blood should not decrease below 8 degrees C because of a spontaneous precipitation of factor VIII from blood (and plasma) below this temperature. (3) Cryoprecipitate derived from rapidly frozen plasma (1 min) contains a decreased amount of proteins in comparison with cryoprecipitate prepared from slowly frozen plasma (45 min to 4 h). On the other hand, equal amounts of factor VIII activity were obtained in the precipitate after freezing of plasma at varying rates. (4) Rapid thawing of plasma results in both higher yields of factor VIII procoagulant activity and a higher specific activity of this factor in the resulting cryoprecipitate. (5) The sedimentation of cryoprecipitate is completed after 5 min centrifugation at 1,500 g. (6) At temperatures higher than 8 degrees C, cryoprecipitated factor VIII starts dissolving into the supernatant plasma or in buffer. (7) Factor VIII in lyophilized cryoprecipitate is stable at room temperature. At elevated temperatures it rapidly looses its activity. (8) Evidence was obtained that the improvements which are introduced in the preparation of factor VIII do not lead to a product which is less stable in vitro as well as in vivo.

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Preparation and Infusion of Cryoprecipitate from Exercised Donors

Cited by 14 publications

References 11 publications

Partial Blockade of Nitric Oxide Synthase Blunts the Exercise-induced Increase of von Willebrand Factor Antigen and of Factor VIII in Man

Partial Blockade of Nitric Oxide Synthase Blunts the Exercise-induced Increase of von Willebrand Factor Antigen and of Factor VIII in Man

Survival of 125iodine-labeled Factor VIII in normals and patients with classic hemophilia. Observations on the heterogeneity of human Factor VIII.

Contributions to the Optimal Use of Human Blood

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