Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nanofiber membrane

Xue, Jiajia; He, Min; Niu, Yuzhao; Líu, Hao; Crawford, Aileen; Coates, Phil; Chen, Dafu; Shi, Rui; Zhang, Liqun

doi:10.1016/j.ijpharm.2014.09.026

Cited by 108 publications

(75 citation statements)

References 41 publications

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“…11,21,22 The higher surface area-to-volume ratio of electrospun fibers has been shown to adsorb vitronectin and fibronectin to facilitate cell adhesion and proliferation. 15,22 As expected, we found that cells were well attached to the PDLLA nanofibrous layer and exhibited equivalent viability as those on the control surface ( Figure 2). To further promote healing and regeneration, MTZ was encapsulated in the outer functional layer at a dosage effective to inhibit periodontopathogen, whereas PDGF was encapsulated in the inner functional layer to promote mitogenesis.…”

Section: Discussionsupporting

confidence: 80%

“…To further promote healing and regeneration, MTZ was encapsulated in the outer functional layer at a dosage effective to inhibit periodontopathogen, whereas PDGF was encapsulated in the inner functional layer to promote mitogenesis. 11,[14][15][16] The sustained release of MTZ and PDGF ( Figure 1E) inhibited of biofilm formation 23 and enhanced the chemotaxis of MSCs, 24 suggesting that FGM may exert beneficial effects in vivo. …”

Section: Discussionmentioning

confidence: 97%

“…Poly(L-lactideco-D/L-lactide) (PDLLA; 70/30, IV: 1.6, 70LDLP200, Green Square Material Inc., Taiwan) at 6 wt % was dissolved in dichloromethane (DCM)/1,4-dioxane 4:6 (v:v) with the addition of 3% MTZ, 0.3% (w/w) PDGF, or 0.3% bovine serum albumin (BSA, in replacement of PDGF for the property assessment of functional layer), and stirred continuously for 24 hours. The dose of MTZ has been shown to inhibit Gram-negative periodontopathogens in vitro, 15 and the dose of PDGF has been shown to promote dentoalveolar regeneration in vivo. 16 The tip-to-collector distance was 10 cm, …”

Section: Fabrication Of the Fgmmentioning

confidence: 99%

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PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration

Ho¹,

Chang²,

Chang³

et al. 2017

IJN

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This study aimed to develop a functionally graded membrane (FGM) to prevent infection and promote tissue regeneration. Poly( l -lactide-co- d , l -lactide) encapsulating platelet-derived growth factor (PDLLA-PDGF) or metronidazole (PDLLA-MTZ) was electrospun to form a nanofibrous layer on the inner or outer surface of a clinically available collagen membrane, respectively. The membrane was characterized for the morphology, molecule release profile, in vitro and in vivo biocompatibility, and preclinical efficiency for alveolar ridge regeneration. The PDLLA-MTZ and PDLLA-PDGF nanofibers were 800–900 nm in diameter, and the thicknesses of the functional layers were 20–30 μm, with sustained molecule release over 28 days. All of the membranes tested were compatible with cell survival in vitro and showed good tissue integration with minimal fibrous capsule formation or inflammation. Cell proliferation was especially prominent on the PDLLA-PDGF layer in vivo. On the alveolar ridge, all FGMs reduced wound dehiscence compared with the control collagen membrane, and the FGM with PDLLA-PDGF promoted osteogenesis significantly. In conclusion, the FGMs with PDLLA-PDGF and PDLLA-MTZ showed high biocompatibility and facilitated wound healing compared with conventional membrane, and the FGM with PDLLA-PDGF enhanced alveolar ridge regeneration in vivo. The design represents a beneficial modification, which may be easily adapted for future clinical use.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 97%

Section: Fabrication Of the Fgmmentioning

confidence: 99%

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PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration

Ho¹,

Chang²,

Chang³

et al. 2017

IJN

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“…Theoretically, the LA incorporated GBR membrane would easily disrupt and kill Gram-positive periodontal bacteria due to absence of an outer membrane which can prevent the influx of foreign molecules 24) . Clinically, oral administration of antibiotics such as metronidazole benzoate (MET) as an adjunctive treatment for periodontal diseases has reduced the presence of pathogens in gingival fluid and periodontal pocket 25) . Furthermore, a PLGA membrane loaded with MET fabricated by a solvent casting method showed a significant improvement on periodontal regeneration 25) .…”

Section: Discussionmentioning

confidence: 99%

“…Clinically, oral administration of antibiotics such as metronidazole benzoate (MET) as an adjunctive treatment for periodontal diseases has reduced the presence of pathogens in gingival fluid and periodontal pocket 25) . Furthermore, a PLGA membrane loaded with MET fabricated by a solvent casting method showed a significant improvement on periodontal regeneration 25) . However, the effectiveness of antibiotic will be reduced when the survived bacteria are continuously proliferate and become resistance toward the administered antibiotic 9,10) .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial efficacy of triple-layered poly(lactic-<i>co</i>-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria

et al. 2017

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A guided bone regeneration (GBR) membrane has been extensively used in the repair and regeneration of damaged periodontal tissues. One of the main challenges of GBR restoration is bacterial colonization on the membrane, constitutes to premature membrane degradation. Therefore, the purpose of this study was to investigate the antibacterial efficacy of triple-layered GBR membrane composed of poly(lactic-co-glycolic acid) (PLGA), nanoapatite (NAp) and lauric acid (LA) with two types of Gram-negative periodontal bacteria, Fusobacterium nucleatum and Porphyromonas gingivalis through a disc diffusion and bacterial count tests. The membranes exhibited a pattern of growth inhibition and killing effect against both bacteria. The increase in LA concentration tended to increase the bactericidal activities which indicated by higher diameter of inhibition zone and higher antibacterial percentage. It is shown that the incorporation of LA into the GBR membrane has retarded the growth and proliferation of Gram-negative periodontal bacteria for the treatment of periodontal disease.

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The Role of Osteopromotive Membranes in Guided Bone Regeneration

Calciolari¹,

Akcalı²,

Donos³

2020

Bone Augmentation by Anatomical Region

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Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nanofiber membrane

Cited by 108 publications

References 41 publications

PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration

PDGF-metronidazole-encapsulated nanofibrous functional layers on collagen membrane promote alveolar ridge regeneration

Antibacterial efficacy of triple-layered poly(lactic-<i>co</i>-glycolic acid)/nanoapatite/lauric acid guided bone regeneration membrane on periodontal bacteria

The Role of Osteopromotive Membranes in Guided Bone Regeneration

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