Preparation and in vivo bacteriostatic application of PPDO-coated Ag loading TiO2 nanoparticles

Abstract

Implant-associated infections limit the clinical application of implants therapy; hence, exploiting strategies to prevent biomaterial-associated infections has become important. Therefore, in this study, a series of poly (p-dioxanone) (PPDO)-coated Ag loading TiO2 nanoparticles (Ag@TiO2-PPDO) was synthesized to be applied as bacteriostatic coating materials that could be easily dispersed in organic solvent and coated onto implantable devices via temperate methods such as electrospraying. The lattice parameters of TiO2 were a = 0.504 nm, b = c = 1.05 nm, alpha = beta = gamma = 90 degree and the size of crystallite was about 13 nm, indicating that part of Ag has been embedded into crystal defects of TiO2. Both XRD and TEM determinations indicated the successful grating of PPDO on the surface of Ag@TiO2. Among Ag@TiO2 nanoparticles with various Ag loading quantities, 12% Ag@TiO2 nanoparticles exhibited relatively higher grafting efficiency and Ag contents on the surface of grafted composites. In addition, 12% Ag@TiO2-PPDO exhibited the best bacteriostatic effect in vitro owing to its higher grafted efficiency and relatively short length of PPDO segments. Subsequently, Ag@TiO2-PPDO was coated on the surface of a poly lactic-co-glycolic acid (PLGA) electrospun membrane via the electrospraying method. Finally, the in vivo bacteriostatic effect of 12% Ag@TiO2-PPDO coating was verified by implanting 12% Ag@TiO2-PPDO-coated PLGA membrane into a rat subcutaneously combined with an injection of Staphylococcus aureus at implanting sites.

Smart-Temporary-Film-Based Local-Delivery System with Controllable Drug-Release Behavior

“…As seen in Figure 4 B, all the samples showed quite similar peaks at 2 θ of 21.8° and 23.5°. PPDO exhibited the characteristic diffraction peaks at 2 θ of 21.9° and 23.7°, which attribute to the reflection of (210) and (020) [ 25 , 26 ], while PCL shows two characteristic peaks at 2 θ of 21.2° and 23.5°, which correspond to the (110) and (200) crystallographic planes of the PCL crystalline structure [ 18 ]. Therefore, the diffraction peaks for all the samples in this work should be the mixture of the characteristic peaks of PPDO and PCL domains.…”

Bacteriostatic effect of Ag@TiO2-Poly(p-dioxanone)-coated gauzes in vitro and in vivo on otitis media pathogens

“…Hexafluoroisopropanol was purchased from Innochem (Beijing, China). NPs of 12%Ag@TiO 2 -PPDO and 16%Ag@TiO 2 -PPDO were synthesized as reported in our previous work [ 11 ]. Xenon lamp equipped with UVCut 420 optical filter, which could filter out light below 420 nm was purchased from China education Au-light (Beijing, China).…”

“…In our previous jobs, a kind of poly( p -dioxanone) (PPDO) grafted Ag-incorporated TiO 2 nanoparticles (Ag@TiO 2 -PPDO NP) has been successfully synthesized. Ag@TiO 2 -PPDO NP could inhibit the proliferation of Staphylococcus aureus [ 11 ]. Spectroscopic studies have shown that Ag@TiO 2 -PPDO NP could respond to visible light, which might make Ag@TiO 2 -PPDO NP inhibit the proliferation of bacteria continually and positively with irradiation of visible light.…”