Preparation and In Vitro Characterization of Chitosan Nanoparticles and Their Broad-Spectrum Antifungal Action Compared to Antibacterial Activities against Phytopathogens of Tomato

Abstract

The present study was to prepare chitosan nanoparticles (CNPs) from chitosan (CS) to evaluate their in vitro antimicrobial activities against phytopathogens of tomato. We prepared and characterized CNPs for their particle size, polydispersity index, and structures. The antifungal properties of CS and CNPs against phytopathogenic fungi namely Colletotrichum gelosporidies, Phytophthora capsici, Sclerotinia sclerotiorum, Fusarium oxysporum, Gibberella fujikuori were investigated. CNPs showed the maximum growth inhibitory effects on mycelial growth of F. oxysporum followed by P. capsici. We also studied antibacterial activities against phytopathogenic bacteria, such as three strains of Erwinia carotovora subsp. carotovora and one strain of Xanthomonas campestris pv. vesicatoria. Our results showed that both CS and CNPs markedly inhibited the growth of the both Xanthomonas and Erwinia strains. From our study, it is evident that both CS and CNPs have tremendous potential against phytopathogens of tomato for further field screening towards crop protection.

Boosting photosynthetic machinery and defense priming with chitosan application on tomato plants infected with Fusarium oxysporum f. sp. lycopersici

“…In our research, chitosan exerts antifungal activity in vitro. These results coincide with those reported by Oh et al (2019), who found that both chitosan and nanoparticles derived from it can be used effectively against phytopathogenic fungi when evaluating the antifungal and antibacterial action in tomato phytopathogens. Conversely, Soliman and El-Mohamedy, (2017) and El-Mohamedy et al (2019) found that HMW and LMW chitosan effectively inhibited the growth of fungal pathogens and that this inhibition increased depending on the concentration.…”

Influence of chitosan nanoparticles and fennel essential oils (Foeniculum vulgare) on the shelf life of Huso huso fish fillets during the storage

“…Poly‐dispersity index (PDI) is another parameter to evaluate the stability of nanoparticles, and it decreased by the concentration of the chitosan such that the PDI for the 1% and 1.5% chitosan was 0.54 and 0.32, respectively. Oh, Chun, and Chandrasekaran () observed the size of CNPs was in the diameter range of 100–1,000 nm and they also reported the potential antimicrobial activity of CNPs. Increase in CNPs size as a function of chitosan concentration was also observed by Gan et al ().…”

“…Increase in CNPs size as a function of chitosan concentration was also observed by Gan et al (). The method of preparing the nano‐chitosan can affect the PDI and stability of nanoparticles (Oh et al, ).…”

Silver Nanoparticles Ecofriendly Synthesized by Achyranthes aspera and Scoparia dulcis Leaf Broth as an Effective Fungicide

“…Recently, Oh et al prepared the chitosan nanoparticles (CNPs) through ionic gelation. These obtained CNPs exhibited the highly antifungal ability against Fusarium oxysporum and Phytophthora capsici, and the good antibacterial effects against Xanthomonas and Erwinia strains [12]. The polymeric nanoparticles achieved a great biocompatibility and antifungal ability, but the degradation time was limited.…”