Preparation and identification of human breast epithelial cells in culture

Easty, G. C.; Easty, D.M.; Monaghan, Paul; Ormerod, M.G.; Neville, A. Munro

doi:10.1002/ijc.2910260509

Cited by 51 publications

(27 citation statements)

References 8 publications

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“…Human mammary glands and benign tumors have also been digested with collagenase and cultured in a way virtually identical to that of the corresponding rat tissues [108][109][110][111]. The organoids so produced are fully capable of yielding all the major cell types in the nude mouse after appropriate hormonal stimulation [112], and of producing very similar cellular morphologies and structures to those of the corresponding rat mammary tissues on plastic [108,109,111] and collagen gels [113][114][115].…”

Section: (6) Culture Of Normal Human Mammary Glands and Their Immortamentioning

confidence: 99%

“…The organoids so produced are fully capable of yielding all the major cell types in the nude mouse after appropriate hormonal stimulation [112], and of producing very similar cellular morphologies and structures to those of the corresponding rat mammary tissues on plastic [108,109,111] and collagen gels [113][114][115]. They therefore consist of epithelial cells ( Fig.…”

Section: (6) Culture Of Normal Human Mammary Glands and Their Immortamentioning

confidence: 99%

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Stem cells and the development of mammary cancers in experimental rats and in humans

Rudland

1987

Cancer Metast Rev

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Evidence based on immunocytochemical staining and ultrastructure suggests that morphological gradations between epithelial and myoepithelial cells, and possibly between epithelial cells and alveolar-like cells occur in terminal ductal structures of rat and human mammary glands. The benign carcinogen-induced rat and benign human mammary tumors can contain epithelial, myoepithelial-like and alveolar-like cells, whereas the malignant counterparts mainly contain only epithelial-like cells. Clonal epithelial cell lines from normal rat mammary glands, benign tumors, and SV40-transformed human mammary glands can differentiate to either myoepithelial-like or alveolar-like cells. In those of the rat, the differentiation processes occur in steps: intermediate cells along the myoepithelial-like pathway resemble intermediates in terminal ductal structures in vivo, and can also generate certain well-differentiated mesenchymal elements of the original tumours. Differentiation of the benign rat cells to alveolar-like cells with mammatrophic hormones and retinoids in vitro leads to a reduction in their tumor-forming ability in vivo. Cell lines from malignant rat mammary tumors of increasing metastatic potential and from human ductal carcinomas largely fail to yield myoepithelial-like or alveolar-like cells and are relatively slow-growing. Growth of the rat mammary epithelial cells in culture is stimulated by a pituitary-derived mammatrophic growth factor (PMGF), prostaglandin E2, and alpha-transforming growth factor; the response of the malignant cell lines to PMGF is reduced. It is suggested that stem cells exist in the rat and human glands that are capable of differentiating to the other major cell types of the mammary parenchyma, and that during the carcinogenic process they generate genetically unstable cells which lose their ability to differentiate and attempt to maximise their intrinsically slow growth rate.

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Section: (6) Culture Of Normal Human Mammary Glands and Their Immortamentioning

confidence: 99%

Section: (6) Culture Of Normal Human Mammary Glands and Their Immortamentioning

confidence: 99%

Stem cells and the development of mammary cancers in experimental rats and in humans

Rudland

1987

Cancer Metast Rev

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“…In part the difficulty arises because traditional culture methods using serum lead to overgrowth of fibroblasts which rapidly outstrip the epithelial elements. This can be minimised by the use of serum-free medium or by a reduction of the concentration of serum to under 1% [10]. An additional problem is that the turnout frequently contains some normal breast epithelium which grows readily in tissue culture, and it is therefore difficult to determine whether the epithelium grown is malignant or benign [4].…”

Section: Introductionmentioning

confidence: 99%

Long-term culture of primary breast cancer in defined medium

McCallum

Lowther

1996

Breast Cancer Res Tr

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Over a period of 6 1/2 years between January 1986 and May 1992, 135 unselected primary breast cancers were cultured and of these 10 developed into cell lines. Six of the lines grew in defined serum-free medium, while the other four required supplementation with 0.5% fetal calf serum. Two of the lines are from the same breast, being derived from a local excision specimen and from a mastectomy specimen 12 months later. In addition, 12 lymph nodes containing metastatic breast cancer were cultured; one of these cultures became permanent in a defined serum-free medium. Oestrogen receptor (ER) status was negative in all but one of the tumours which grew successfully, and even in this case the derived cell line is ER negative. The epithelial nature of the lines has been confirmed by immunocytochemistry and by electron microscopy (EM), while their malignant nature is shown by morphology, unattached growth, chromosome analysis, and, in the case of the line from a lymph node metastasis, the absence of any benign source of epithelial cells.

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“…HMECs were prepared as organoids (clusters of luminal and myo-epithelial cells) by digestion of human mammary tissue removed at reduction mammoplasty (21,22). Briefly, 200-400 g of breast tissue were minced and digested using progressive collagenase digestion (type 1, Sigma), followed by sedimentation and sequential filtration through 140 µm and then 53 µm nylon mesh.…”

Section: Cell Culture and Carcinogen Treatmentmentioning

confidence: 99%