A novel CrCx/C-Cr multilayer coating for cotton picking spindle was prepared on 20CrMnTi substrate by PVD technology. Compared with the traditional electroplated chromium coating, the CrCx/C-Cr coating has dense structure, and no micro-cracks were observed on the surface. The results of nanoindentation show that the nano-hardness of CrCx/C-Cr coating is about 8.46 GPa and 78.5% higher than that of the substrate. Compared with electroplated chromium coating, PVD coating has lower friction coefficient and wear rate, which indicates a better wear resistance property. The multilayer PVD coating provided is expected to replace the high-pollution chromium coating for cotton picking spindle.