Preparation and functional properties of probiotic and oat-based synbiotic yogurts fermented with lactic acid bacteria

Lim, Eun Seo

doi:10.1007/s13765-017-0333-5

Cited by 17 publications

(14 citation statements)

References 55 publications

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“…Recently, yogurt has been extensively studied to further enhance its functions using bioactive substances from edible plants. Numerous studies have indicated that the addition of these substances increases the concentration of polyphenols and flavonoids, thus increasing the antioxidant and antimicrobial activities of yogurt ( Halah and Nayra, 2011 ; Kang et al, 2018 ; Lim, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Properties and Diet-Related α-Glucosidase and Lipase Inhibitory Activities of Yogurt Supplemented with Safflower (Carthamus tinctorius L.) Petal Extract

Hong¹,

Lim²,

Kothari³

et al. 2021

Food Sci Anim Resour

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Recently, yogurt has been extensively studied to further enhance its functions using edible plant extracts. This study was conducted to investigate whether safflower petal (SP) as a natural food additive can be used to develop functional yogurt with improved health benefits. SPs were extracted with ethanol (SPE) and hot water (SPW), and then safflower yogurt was prepared by adding 0%–1.0% of those extracts to plain yogurt. With an increase in the fermentation duration, the pH of SPE and SPW yogurt samples was decreased, whereas titratable acidity and microbial counts were increased. The concentration of total polyphenols and total flavonoids, the activity of antioxidants, and the inhibitory effect on reactive oxygen species (ROS) were higher in SPW yogurt than SPE yogurt. Furthermore, α-glucosidase and lipase activity inhibitory effects of SPW yogurt were higher than those of SPE yogurt. In particular, free radical-scavenging activities, ROS inhibitory effect, and α-glucosidase activity inhibitory effects were significantly increased in SPW yogurt in a dose-dependent manner. Overall, these results suggest that SP extract possesses antioxidant activities and that it can downregulate α-glucosidase and lipase activities. The SP extract may have potential benefits as a natural food additive for the development of functional yogurt.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Properties and Diet-Related α-Glucosidase and Lipase Inhibitory Activities of Yogurt Supplemented with Safflower (Carthamus tinctorius L.) Petal Extract

Hong¹,

Lim²,

Kothari³

et al. 2021

Food Sci Anim Resour

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“…Patagonin-CRISP was screened against various strains of microorganisms with liquid growth inhibition assay. The microbial strains chosen are drawn from snakes' oral cavities or are of medical and economic relevance (Hetru and Bulet, 1997;Vassilara et al, 2017;Yin et al, 2017;Ghosh et al, 2018;Yadav et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)

et al. 2021

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Snake venom contains a variety of toxins with a range of biological activity, among these toxins cysteine-rich secreted proteins (CRISPs) can be found. The proteins of this family have masses of 20–30 kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disulfide bonds. Some of these proteins have been explored, characterized, and described in terms of their activity; however, little is known about their range of activities. A search for new antimicrobial molecules is ongoing, as the number of microbial strains resistant to available antibiotics is increasing. We identified antimicrobial activity in the secretion of Duvernoy's gland of the rear-fanged Philodryas patagoniensis. Fractions of this venom were subjected to reverse-phase high performance liquid chromatography and analyzed to determine their antimicrobial activity with a liquid broth inhibition assay. One of the fractions presented activity against a Gram-negative bacterium and a filamentous fungus. This fraction was analyzed with LC-MS/MS, and a protein of 24,848.8 Da was identified. Database searches allowed us to identify it as a CRISP due to the presence of some unique fragments in the molecule. We called it patagonin-CRISP, as the same protein in the venom of P. patagoniensis had previously been characterized as having a different biological activity. Patagonin-CRISP presented activity at very low concentrations and showed no cytotoxic activity. This is the first time that antimicrobial activity has been identified for P. patagoniensis venom or for a CRISP family protein.

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“…Each 1 g of the broken pieces received 9 mL of PBS solution to be shaken gently a few times. Then, each of the PBS solution was serially diluted, to be spread onto LB agar to count the colony forming unit (CFU) [13,14], by which immobilizing capacity (CFU/g of dry EC) of expanded clay was calculated.…”

Section: Performance Tests Of Bacteria-immobilized Expanded Clay As Cmentioning

confidence: 99%

Effectiveness of expanded clay as a bacteria carrier for self-healing concrete

et al. 2019

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Cracking of concrete over time, is a natural phenomenon. Longer service life of concrete structures is desirable. Self-healing concrete using bacteria, which could form CaCO 3 crystals for crack sealing, has promised benefits to reduce cost for concrete maintenance, because cracks could be autonomously repaired without human intervention. However, because of harsh concrete internal environment render the effectiveness depending on the bacteria viability within concrete. In this study, expanded clay (EC) was used as a carrier, to protect bacteria (Lysinibacillus boronitolerans YS11) from the harsh environment during the process. Existence of bacteria inside EC was observed using electron microscopy. When exposed to bacterial solution of 1.0 × 10 9 cells/mL, bacterial density within EC reached approximately 0.82 × 10 7 cells/g of dry EC. Extent of bacterial viability within EC, submerged to solution containing 1.0 × 10 8 cells/mL, was 53.6% of free bacteria solution containing 1.0 × 10 7 cells/mL, as measured with fluorescein diacetate assay. When rate of calcium carbonate formation was measured with Ca 2+ disappearance, rates were comparable between bacteria within EC (submerged to bacterial solution containing 1.0 × 10 8 cells/mL) and free bacteria (1.0 × 10 7 cells/mL). This finding indicates that bacteria with EC is very active for generation of CaCO 3 within EC. All experimental results suggest that EC may be an adequate bacteria carrier for self-healing concrete.

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Preparation and functional properties of probiotic and oat-based synbiotic yogurts fermented with lactic acid bacteria

Cited by 17 publications

References 55 publications

Antioxidant Properties and Diet-Related α-Glucosidase and Lipase Inhibitory Activities of Yogurt Supplemented with Safflower (Carthamus tinctorius L.) Petal Extract

Antioxidant Properties and Diet-Related α-Glucosidase and Lipase Inhibitory Activities of Yogurt Supplemented with Safflower (Carthamus tinctorius L.) Petal Extract

Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)

Effectiveness of expanded clay as a bacteria carrier for self-healing concrete

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