Preparation and Functional Characterization of Decellularized Bovine Tendon Scaffolds for Tendon Tissue Engineering

Bhat, Manzoor A.; Zama, Mms; Batoo, Azhar Shuaib; Javaid, Moien; Pathak, Raghuvar D.; Suvarna, A; Hoque, Mozammel; Singh, Rajendra K.; Sharma, Neha; Rashmi, Rashmi

doi:10.5958/2277-940x.2015.00152.7

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…The spindle‐shaped RADMSCs showed more proliferation on DT scaffolds. This observation corroborates with the earlier report of cell‐seeded scaffolds, with their 3D surface topography being an ideal biomimetic niche for RADMSCs growth 66 …”

Section: Discussionsupporting

confidence: 93%

“…This observation corroborates with the earlier report of cell-seeded scaffolds, with their 3D surface topography being an ideal biomimetic niche for RADMSCs growth. 66 Thus, the search for an ideal biomimetic tendon scaffold synergic with a cost-effective drug delivery system has led to the design of this study. This quest was further satisfied with the obtained results of various experiments carried out at the fabrication, characterization and efficacy evaluation levels.…”

Section: Discussionmentioning

confidence: 99%

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Natural 3D Extra Cellular Matrix mimicking stem cells seeded decellularized scaffolds as a platform for tendon regeneration

John

Joseph

et al. 2023

J Biomed Mater Res

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Achilles tendon, which connects the calf muscles to heel, is the strongest tendon in the body. Despite its strength, it is more prone to injury due to its limited blood supply. Tendon-related injuries are more common in sportspersons, people with laborintensive work and the aged community. The currently available treatment mode is surgery which is expensive with chances of re-injury. Present study made an attempt to fabricate a tissue-engineered tendon product using decellularized tendon (DT) seeded with stem cells and bioactive components of Tinospora cordifolia extract (TCE). The bare DT tissue scaffold/substitute may also serve as a drug delivery platform for growth factors and cells with a new approach to promote tissue regeneration in clinical applications. DT construct showed good regenerative potential and easily promoted new tissue formation. Decellularization of the tendon was carried out by chemical method using tri (n-butyl) phosphate (TnBP). DT was physicochemically characterized by contact angle measurement, thermal gravimetric analysis (TGA), and mechanical testing. Rabbit adipose derived mesenchymal stem cells (RADMSCs) were isolated and phenotypically characterized by flow cytometry analysis, tri lineage differentiation, and so forth. Further, stem cell seeded DT scaffolds were prepared and found to be non-toxic by cytotoxicity, cell adhesion by scanning electron microscope (SEM) analysis, cell viability by live dead assays, and so forth.The findings of this study yield valid proof for the employability of cell-seeded DT construct as a natural scaffold in repairing injured tendons-the toughest chords of the skeleton. This is a cost effective method for the replacement of injured/damaged tendons for athletes, people in labor-intensive occupations, the elderly population, and so forth-a boon towards the repair of the tendon in damage/injury.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Natural 3D Extra Cellular Matrix mimicking stem cells seeded decellularized scaffolds as a platform for tendon regeneration

John

Joseph

et al. 2023

J Biomed Mater Res

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“…[129], [130], bovine [24], [102], [131], [132], equine [133], [134] and porcine [22], [81], [103]- [105], [135], [136]. It is important to select the appropriate animal source, as each animal has its pros and cons.…”

Section: I41 Decellularization Methodsmentioning

confidence: 99%

Writing 3D In Vitro Models of Human Tendon within a Biomimetic Fibrillar Support Platform

Monteiro¹,

Bakht²,

Gomez‐Florit³

et al. 2023

ACS Appl. Mater. Interfaces

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Tendinopathies are poorly understood diseases for which treatment remains challenging. Relevant in vitro models to study human tendon physiology and pathophysiology are therefore highly needed. Here we propose the automated 3D writing of tendon microphysiological systems (MPSs) embedded in a biomimetic fibrillar support platform based on cellulose nanocrystals (CNCs) self-assembly. Tendon decellularized extracellular matrix (dECM) was used to formulate bioinks that closely recapitulate the biochemical signature of tendon niche. A monoculture system recreating the cellular patterns and phenotype of the tendon core was first developed and characterized. This system was then incorporated with a vascular compartment to study the crosstalk between the two cell populations. The combined biophysical and biochemical cues of the printed pattern and dECM hydrogel were revealed to be effective in inducing human-adipose-derived stem cells (hASCs) differentiation toward the tenogenic lineage. In the multicellular system, chemotactic effects promoted endothelial cells migration toward the direction of the tendon core compartment, while the established cellular crosstalk boosted hASCs tenogenesis, emulating the tendon development stages. Overall, the proposed concept is a promising strategy for the automated fabrication of humanized organotypic tendon-on-chip models that will be a valuable new tool for the study of tendon physiology and pathogenesis mechanisms and for testing new tendinopathy treatments.

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“…Acellular tendon grafts were developed from bubaline tendon by processes including combination of physical and chemical treatment [4]. Terminal sterilization was done by UV irradiation.…”

Section: Methodsmentioning

confidence: 99%

Clinical Management of Achilles Tendon Injury with Tendon Gap Defect in a Horse Using Tissue Engineering: A Case Report

Wahengbam¹,

Pathak²,

Hajam³

et al. 2023

Act Sci VetSci

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A 3 years old horse was presented to Referral Veterinary Polyclinic I.V.R.I with history of traumatic injury on left hind limb with lameness. The case was examined and diagnosed as lacerated and crushed rupture of Achilles tendon with tendon gap defect. With suitable anaesthetic protocol and aseptic measures, the tendon grafting was performed using a tested tissue engineered bubaline derived tendon grafts to reconstruct the tendon. Postoperatively the limb was immobilized with fiberglass cast and antibiotic coverage along with anti-inflammatory and routine dressing. However, infection developed after few days, which might have been due to open wound presented, resulting in the removal of the graft. Hence, the treatment of the tendon gap defect wound was attempted with bubaline tendon derived collagen gel application resulting in a progressive healing. The fiberglass cast with splint were also removed 25 days post-surgery. Thereafter, a moderate weight bearing while standing on the affected limb was noticed post collagen gel treatment. The animal regained normal weight bearing and made an uneventful recovery after three months.

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Preparation and Functional Characterization of Decellularized Bovine Tendon Scaffolds for Tendon Tissue Engineering

Cited by 4 publications

References 18 publications

Natural 3D Extra Cellular Matrix mimicking stem cells seeded decellularized scaffolds as a platform for tendon regeneration

Natural 3D Extra Cellular Matrix mimicking stem cells seeded decellularized scaffolds as a platform for tendon regeneration

Writing 3D In Vitro Models of Human Tendon within a Biomimetic Fibrillar Support Platform

Clinical Management of Achilles Tendon Injury with Tendon Gap Defect in a Horse Using Tissue Engineering: A Case Report

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