Preparation and extensive characterization of hyaluronan with narrow molecular weight distribution

Čožíková, Dagmar; Šílová, Tereza; Moravcová, Veronika; Šmejkalová, Daniela; Pepeliaev, Stanislav; Velebný, Vladimı́r; Hermannová, Martina

doi:10.1016/j.carbpol.2016.12.045

Cited by 32 publications

(7 citation statements)

References 33 publications

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“…Therefore, alternative characteristic mean values with higher sensitivity for in particular narrow distributions have been introduced. For our study we have adapted the concept of the cumulative distribution angle 84 originally proposed for molar mass distributions to be used for the comparison of ST distributions. The distribution angle is expected to display changes in the steep region of the cumulative ST distribution.…”

Section: Thermophoretic Analysismentioning

confidence: 99%

Impact of Electron Beam Irradiation on Thermoplastic Polyurethanes Unraveled by Thermal Field-Flow Fractionation

Geisler

Pal

Arnhold

et al. 2021

Polymer Degradation and Stability

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The impact of electron beam irradiation on thermoplastic polyurethane material was studied for both an aliphatic and an aromatic polyurethane with equal amount of hard and soft segments. Irradiation doses up to 300 kGy at room temperature and at 100 °C were applied. Changes in chemical structure, molar mass and size were assessed using infrared spectroscopy, differential scanning calorimetry, size exclusion chromatography and thermal field flow fractionation. Material alterations were correlated with trends regarding to degradation, crosslinking or branching changes. Thereby, limits of characterization by size exclusion chromatography are addressed and amended by thermal field-flow fractionation studies. In addition, a thermophoretic analysis has been carried out complementary to the portfolio of analytical methods applied in this work.

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Section: Thermophoretic Analysismentioning

confidence: 99%

Impact of Electron Beam Irradiation on Thermoplastic Polyurethanes Unraveled by Thermal Field-Flow Fractionation

Geisler

Pal

Arnhold

et al. 2021

Polymer Degradation and Stability

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show abstract

“…For all the above-mentioned reasons, it is important to identify the Mw of the polysaccharide (Braithwaite et al, 2016 ). A good method for the determination of the average molecular weight of native and modified HA is size exclusion chromatography combined with multiangle light scattering (SEC-MALLS) (Cožíková et al, 2017 ). Hydrodynamic characterization of HA and commercial products were characterized using SEC-TDA (Size Exclusion Chromatography-Triple Detector Array) (Salzillo et al, 2016 ).…”

Section: Physico-chemical Characterization Of Native Hamentioning

confidence: 99%

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Huerta-Ángeles

Nešporová

Ambrožová

et al. 2018

Front. Bioeng. Biotechnol.

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This review shows the steps toward material selection focalized on the design and development of medical devices based on hyaluronan (HA). The selection is based on chemical and mechanical properties, biocompatibility, sterilization, safety, and scale-up costs. These facts play a vital role in the industrialization process. Approved medical devices containing-HA are illustrated to identify key parameters. The first part of this work involves the steps toward a complete characterization of chemical and mechanical aspects, reproducibility of the processes and scale up. In a second stage, we aimed to describe the preclinical in vitro and in vivo assays and selected examples of clinical trials. Furthermore, it is important to keep in mind the regulatory affairs during the research and development (R&D) using standardization (ISO standards) to achieve the main goal, which is the functionality and safety of the final device. To keep reproducible experimental data to prepare an efficient master file for the device, based on quality and recorded manufacturing data, and a rigorous R&D process may help toward clinical translation. A strong debate is still going on because the denominated basic research in HA field does not pay attention to the purity and quality of the raw materials used during the development. So that, to achieve the next generation of devices is needed to overcome the limitations of state of art in terms of efficacy, biodegradability, and non-toxicity.

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“…As oxidation agents mainly ozone (Yue 2012;Rother et al, 2015) or chlorine dioxide (Wyrwa et al, 2012) have been used. In recent years, intensive studies have been performed about acidic degradation of HA (Smejkalová et al, 2012a;Cozíková et al, 2017). Degraded HA with very narrow dispersity (Mw/Mn < 1.23) covering a broad range of differently sized products (420-3 kDa) could thus be obtained prior fractionation on a preparative scale.…”

Section: Controlled Degradation To Provide Suitable Starting Hyaluron...mentioning

confidence: 99%

Chemical Modification of Hyaluronan and Their Biomedical Applications

2022

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Hyaluronan, the extracellular matrix glycosaminoglycan, is an important structural component of many tissues playing a critical role in a variety of biological contexts. This makes hyaluronan, which can be biotechnologically produced in large scale, an attractive starting polymer for chemical modifications. This review provides a broad overview of different synthesis strategies used for modulating the biological as well as material properties of this polysaccharide. We discuss current advances and challenges of derivatization reactions targeting the primary and secondary hydroxyl groups or carboxylic acid groups and the N-acetyl groups after deamidation. In addition, we give examples for approaches using hyaluronan as biomedical polymer matrix and consequences of chemical modifications on the interaction of hyaluronan with cells via receptor-mediated signaling. Collectively, hyaluronan derivatives play a significant role in biomedical research and applications indicating the great promise for future innovative therapies.

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Preparation and extensive characterization of hyaluronan with narrow molecular weight distribution

Cited by 32 publications

References 33 publications

Impact of Electron Beam Irradiation on Thermoplastic Polyurethanes Unraveled by Thermal Field-Flow Fractionation

Impact of Electron Beam Irradiation on Thermoplastic Polyurethanes Unraveled by Thermal Field-Flow Fractionation

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Chemical Modification of Hyaluronan and Their Biomedical Applications

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