Preparation and evaluation of the in vitro drug release properties and mucoadhesion of novel microspheres of hyaluronic acid and chitosan

Lim, S. T.; Martin, Gary P.; Berry, David J.; Brown, Marc

doi:10.1016/s0168-3659(99)00285-0

Cited by 256 publications

(108 citation statements)

References 30 publications

Supporting

Mentioning

104

Contrasting

Unclassified

Order By: Relevance

“…This technique has been already utilized in the research of microcapsules [25] and has many advantages as follows: (1) easy size control by controlling of the flow rate of liquid in the order of micrometers [26], (2) small variations in their size compared with droplet preparation methods using a magnetic stirrer and a homogenizer [27,28], and (3) easy gelation due to modification by the phenol moiety and gelation by the peroxidase-catalyzed reaction in the laminar flow [29] because elemental HA cannot be gelated. This adjustable size range and molecular polymerization in the co-flowing system enabled the development of HA microcapsules of similar size to embryos.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures

Tomita

Sakai

Khanmohammadi

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose We investigated whether enzymatically fabricated hyaluronan (HA) microcapsules were feasible for use in the cryopreservation of a small number of sperm. Methods HA microcapsules were fabricated using a system of water-immiscible fluid under laminar flow. Three sperm were injected into a hollow HA microcapsule using a micromanipulator. Capsules containing injected sperm were incubated in a freezing medium composed of sucrose as the cryoprotectant and then placed in a Cryotop® device and plunged into liquid nitrogen. After thawing, the capsule was degraded by hyaluronidase, and the recovery rate of sperm and their motility were investigated. Results The HA microcapsule measuring 200 μm in diameter and with a 30-μm thick membrane was handled using a conventional intracytoplasmic sperm injection (ICSI) system, and the procedure involved the injection of sperm into the capsule. The HA microcapsules containing sperm were cryopreserved in a Cryotop® device and decomposed by the addition of hyaluronidase. The recovery rate of sperm after cryopreservation and degradation of HA microcapsules was sufficient for use in clinical practice (90 %). Conclusions Hollow HA microcapsules can be used for the cryopreservation of a small number of sperm without producing adverse effects on sperm quality.

show abstract

Section: Discussionmentioning

confidence: 99%

Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures

Tomita

Sakai

Khanmohammadi

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…CS has been investigated as a unique vehicle for the sustained delivery of drugs, 4,5) in particular, the preparation of CS microspheres has been studied. [5][6][7] We investigated the preparation of a suitable vehicle, such as microspheres, for intra-articular injection in rheumatoid arthritis to allow sustained drug delivery. Lu et al 8) reported that CS could act on the epiphyseal cartilage and augment wound healing of articular cartilage.…”

mentioning

confidence: 99%

Biodegradation and Drug Release of Chitosan Gel Beads in Subcutaneous Air Pouches of Mice.

Kofuji

Ito

Murata

et al. 2001

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The polysaccharide chitosan (CS) is known to be an excellent material for drug preparation. CS is a plentiful natural biopolymer and is non-toxic, biocompatible and biodegradable.1-3) These properties are important for materials that are implanted in the body, because such materials must avoid the host's defense system during their long-term contact with living structures. CS has been investigated as a unique vehicle for the sustained delivery of drugs, 4,5) in particular, the preparation of CS microspheres has been studied. [5][6][7] We investigated the preparation of a suitable vehicle, such as microspheres, for intra-articular injection in rheumatoid arthritis to allow sustained drug delivery. Lu et al. 8) reported that CS could act on the epiphyseal cartilage and augment wound healing of articular cartilage. Therefore, it might be useful in healing wounds in articular cartilage after fulfilling a role as a vehicle for drug delivery. In a previous study, CS was found to form gel spheres at around pH 9 in amino acid solution, despite usually forming a gel in solutions with a pH Ͼ12.9) This is thought to be due to coacervation. Preparations made at a lower pH are preferable due to their effect on the solubility or stability of the drug contained in the gel beads and on the tissue into which they are injected. Furthermore, the release of drug from CS gel beads could be controlled by the formation of a complex between chondroitin sulfate and CS.10) Intra-articular injection of steroids is used commonly in the treatment of rheumatoid arthritis. Under such conditions, it is difficult to achieve a sustained intra-articular drug level on the basis of drug solubility. During in vivo degradation, drug release is governed by both diffusion and biodegradation of the matrix. It is necessary to clarify the relationship between in vivo biodegradability and drug release profiles of CS gel beads under different conditions (enzymes, dissolution medium, stirring strength, etc.), and those in vitro. In this study, this relationship was investigated by implanting novel CS gel beads into subcutaneous air pouches (AP) prepared on the dorsal surface of mice. MATERIALS AND METHODSMaterials CS with varying degrees of deacetylation (70% (7B), 80% (8B), 90% (9B), 100% (10B)) was purchased from Katokichi Co., Ltd., Japan. Prednisolone (PS) and sodium alginate (300 cps) (Alg) were purchased from Nacalai Tesque Inc., Japan. The other reagents were obtained from Wako Pure Chemical Industries and Nacalai Tesque Inc., Japan.Preparation of CS Gel Beads CS gel beads were prepared as follows: CS (1% w/w) was dissolved in 0.1 M acetate buffer (pH 4.5) and 1% PS was then added to the CS solution. One gram of this suspension, theoretically containing 10 mg of PS, was dropped slowly into 20 ml of 10% glycine solution (pH 9.0) using a pipette and left at room temperature for 25 min. Hydrogel beads were formed spontaneously. Dried gel beads were obtained by drying the hydrogel beads at 37°C for 24 h in a dish, before holding them under vacuum in a d...

show abstract

“…It is estimated that the drug may be associated with vestiges of the positively charged nanoparticles through electrostatic interactions, so lower surface charge favored higher cumulative drug release in PBS, i.e. cumulative drug release of NHT-CS (3:1) was higher than those of NHT-CS (1:1) and NHT-CS (2:1) (Lim et al 2000). as the sample is analysed from receiving compartment where it has diffused from nanoparticles through dialysis membrane.…”

Section: Controlled Drug Release Study By Dialysis Membranementioning

confidence: 99%

“…The rate of release was divided into an initial phase (0-8 hrs) and a terminal phase (8-144hrs) based on a modified report by Lim (S. Lim, Martin, Berry, & Brown, 2000) as it is understandable that the release rate in the burst release stage is faster than that of in the sustained release phase. In an attempt to explore the characteristics of drug release profile, the data were fitted to four different mathematical models, i.e., zero order model, first order model, Higuchi model, and Hixson-Crowell model.…”

Section: Kinetics Of Drug Releasementioning

confidence: 99%

“…The results before mathematic model correction of release rate constants (k) and coefficients of determination (r 2 ) were shown in Table 4.1. The initial phase of release of NHT was faster than that of the terminal phase due to the higher concentration gradient, and this was because of the adherence of the drug on the nanoparticle surface and the defects of pores or cracks within the matrix (S. Lim et al, 2000). As shown in Table 4.1, it indicated that NHT-loaded CS nanoparticles were best characterised by first order model (r 2 =0.9116-0.9680) and Higuchi model (r 2 =0.9796-0.9998) with high linearity.…”

Section: Kinetics Of Drug Releasementioning

confidence: 99%

See 1 more Smart Citation

Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery

Wang¹

View full text Add to dashboard Cite

Cigarette smoking has become one of the leading causes of preventable deaths all over the world, causing a serious threat to human wellbeing and a tremendous economic burden to governments. The currently available treatment options for smoking cessation are not efficient. The pulmonary delivery of drugs by methods such as dry powder inhaler (DPI) has been recognized as one of the most efficient routes of drug delivery to the targeted area. The lung delivery of nicotine in this way would be expected to mimic the effects of tobacco smoking and could significantly reduce the negative health effects of smoking that result from nicotine addiction.The aim of this study is to develop nanoparticles with controlled physicochemical properties using biodegradable polymer of chitosan (CS) loaded with nicotine salt for pulmonary delivery as DPI formulations. The outcomes of this project are expected to be a safe and effective CS-based nicotine formulation for pulmonary delivery to treat nicotine dependence. This thesis specially addresses the research questions: (1) how to develop a feasible process to manufacture the nanoparticles suitable for pulmonary drug delivery using biodegradable polymer of CS containing nicotine salt; (2) how to develop an animal model for pulmonary drug delivery from DPI formulation using a nose-only inhalation device.The current therapeutic options for treatment of smoking addiction have been reviewed, and current advancements of technology in pulmonary drug delivery are summarised. Buccal administration of drugs exhibits a low oral bioavailability, and sublingual tablets and chewing gums can be swallowed before being absorbed.Although nasal spray of nicotine is a fast way to deliver nicotine into the bloodstream, Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery iii the rate of absorption is still not as rapid as cigarette smoking. Therefore, it is proposed that delivery of nicotine with sustained drug release profile direct into the deep lung is likely to more effectively mimic the rapid effects of cigarette smoking on a physiological level, which could eliminate patient craving and allow the tapering of nicotine level over time to improve patients' compliance.Water in oil (w/o) emulsion crosslinking method has been used to fabricate nicotine hydrogen tartrate (NHT)-loaded CS nanoparticles which separate as solid microaggregates. The physicochemical properties of particles are characterized by a series of techniques. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) shows that the prepared particles are spherical in morphology with rough surface after loading CS particles with NHT. Dynamic Light Scattering (DLS)by Zetasizer determined nanoparticle size ranging from 167.6 nm to 411 nm, while DLS by Mastersizer demonstrated that the microaggregates of these nanoparticles are in the respirable range (<5µm). The surface positive charge as measured by zeta potential tends to decrease with increase of mass ratios of NHT to CS, which is in contr...

show abstract

Preparation and evaluation of the in vitro drug release properties and mucoadhesion of novel microspheres of hyaluronic acid and chitosan

Cited by 256 publications

References 30 publications

Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures

Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures

Biodegradation and Drug Release of Chitosan Gel Beads in Subcutaneous Air Pouches of Mice.

Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery

Contact Info

Product

Resources

About