Preparation and Evaluation of the Antibacterial Effect of Magnetic Nanoparticles Containing Gentamicin: A Preliminary In vitro Study

Douzandeh-Mobarrez, Banafsheh; Dogaheh, Mehdi Ansari; Eslaminejad, Touba; Kazemipour, Maryam; Shakibaie, Mojtaba

doi:10.21859/ijb.1559

Cited by 11 publications

(3 citation statements)

References 25 publications

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“…Moreover, the characteristic peaks of GNT can be attributed to the amide bands, bending vibration of S-O, and stretching vibration of S-O, which appeared at 1627, 617, and 1057 cm -1 , respectively. 33,34 G4, G5, and G6: All the characteristic peaks of PCL and GNT, which appeared at the FTIR spectra of G1, G2, and G3, were also observed with a slight shift for these formulations. The characteristic peaks of MP detected at 1450 and 1369 cm -1 can also be related to the CH3 bands.…”

Section: G1 G2 and G3mentioning

confidence: 91%

Design and Development of Antibacterial/Anti-inflammatory Dual Drug-Loaded Nanofibrous Inserts for Ophthalmic Sustained Delivery of Gentamicin and Methylprednisolone: In Vitro Bioassay, Solvent, and Method Effects’ Evaluation

Mirzaeei

Barfar

2021

Adv Pharm Bull

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Purpose: To overcome the challenges caused by the use of conventional ophthalmic dosage forms such as the fast elimination of the drug from the surface of the eye, in this study, dual drug-loaded nanofibers were developed for sustained ophthalmic delivery of Gentamicin (GNT) and Methylprednisolone (MP). Moreover, the solvent effects, polymer mixtures, and method of preparation on the release profile of the prepared nanofibers, were evaluated. Methods: The nanofibers were prepared using Polycaprolactone, Poly (lactic-co-glycolic acid), and Polyvinyl alcohol using electrospinning technique. Thereafter, seven optimized formulations were developed with different solvent mixtures and polymer concentrations using various electrospinning methods. The physicochemical and mechanical properties of nanofibers were also evaluated, and the morphology of formulations was observed. The antibacterial efficacy was investigated and the in vitro release amounts of GNT and MP from nanofibers were estimated using the bioassay and Ultraviolet-visible spectroscopy. Results: The developed G1, G4, G5, G6, and G7 had suitable mechanical properties and morphologies with diameter ranging between 70-350 nm. The 1:1 v/v ratio of DMF/DCM in the solvent mixture and using core-shell technique for the preparation, formed nanofibers with more favorable release profiles. The optimized formulations indicated sustained-release manner for both drugs during 3-9 days and the antibacterial efficacy against Staphylococcus aureus. Conclusion: Among all the prepared formulations, the nanofiber with core-shell structure possessed the best sustained-release profiles of GNT and MP. The obtained results suggest that these nanofibers have a potential to be used as an insert in the eye for long-term release of the drug.

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Section: G1 G2 and G3mentioning

confidence: 91%

Design and Development of Antibacterial/Anti-inflammatory Dual Drug-Loaded Nanofibrous Inserts for Ophthalmic Sustained Delivery of Gentamicin and Methylprednisolone: In Vitro Bioassay, Solvent, and Method Effects’ Evaluation

Mirzaeei

Barfar

2021

Adv Pharm Bull

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“…As antibacterial components in the composition of modifying coatings, nanoparticles of metals and their oxides (Ag, ZnO, CuO, MgO, MnO 2 , TiO 2 , and Fe 2 O 3 ) [6][7][8][9][10][11][12][13] are used; they have high activity against a number of gram-positive and gram-negative bacteria and low cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Modification of nonwoven polymer materials for increasing of their filtration and antibacterial properties

Avdeeva,

Petkevich,

Mikhalko

et al. 2023

Polymer Engineering & Sci

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Polytetrafluoroethylene (PTFE), octenidine dihydrochloride (OCT), and zinc oxide (ZnO) were used to modify the polypropylene nonwoven material by the methods of “wet chemistry” and low‐energy electron beam deposition (EBD). The influence of nonwoven material modification on the morphology, chemical composition, filtration, and antibacterial properties was established. The modified material has antibacterial activity against the gram‐positive strain Staphylococcus aureus and against gram‐negative strains of Escherichia coli and Klebsiella pneumoniae (suppression of bacterial growth for materials with OCT was 100%, with ZnO—70%). PTFE application to Aquaspun leads to a significant increase in the contact angle (from 141.3° to 152.7°) and air filtration efficiency (from 78.3% to 83.4%), which provides the barrier properties of the material. It was established that the material obtained using low‐energy EBD demonstrated a more pronounced antimicrobial potential against the tested strains of St. aureus.Highlights The influence of polypropylene nonwoven materials surface modification methods is systematically investigated. Low‐energy electron beam deposition increases antibacterial and filtration properties of nonwoven materials. Surface modification methods increase barrier properties of polypropylene nonwoven materials.

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“… 13 Moreover, sodium alginate was used as surface modifier on magnetic nanoparticles containing gentamicin for antibacterial targeting therapy. 14 In addition, superparamagnetic nanoparticles coated by alginate/chitosan/β-cyclodextrin were used to purify α-amylase enzyme. 15 Targeted nanoparticles have been used in drug delivery to cancer cells because of their characteristics.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Effects of Curcumin Loaded on Chitosan Alginate Nanospheres on the KMBC-10 Spheroids Cell Line

Afzali¹,

Eslaminejad²,

Rouholamini³

et al. 2021

IJN

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Breast cancer is one of the most lethal types of cancer in women. Curcumin showed therapeutic potential against breast cancer, but applying that by itself does not lead to the associated health benefits due to its poor bioavailability, which appears to be primarily due to poor absorption, rapid metabolism, and rapid elimination. Moreover, poor water solubility of curcumin causes accumulation of a high concentration of curcumin and so decrease its permeability to the cell. Many strategies are employed to reduce curcumin metabolism such as adjuvants and designing novel delivery systems. Therefore, in this study sodium alginate and chitosan were used to synthesize the hydrogels that are known as biocompatible, hydrophilic and low toxic drug delivery systems. Also, folic acid was used to link to chitosan in order to actively targetfolate receptors on the cells. Methods: Chitosan-β-cyclodextrin-TPP-Folic acid/alginate nanoparticles were synthesized and then curcumin was loaded on them. Interaction between the constituents of the particles was characterized by FTIR spectroscopy. Morphological structures of samples were studied by FE-SEM. Release profile of curcumin was determined by dialysis membrane. The cytotoxic test was done on the Kerman male breast cancer (KMBC-10) cell line by using MTT assay. The viability of cells was detected by fluorescent staining. Gene expression was investigated by realtime PCR. Results: The encapsulation of curcumin into nano-particles showed an almost spherical shape and an average particle size of 155 nm. In vitro cytotoxicity investigation was indicated as dose-respond reaction against cancer breast cells after 24 h incubation. On the other hand, in vitro cell uptake study revealed active targeting of CUR-NPs into spheroids. Besides, CXCR 4 expression was detected about 30-fold less than curcumin alone. The CUR-NPs inhibited proliferation and increased apoptosis in spheroid human breast cancer cells. Conclusion: Our results showed the potential of NPs as an effective candidate for curcumin delivery to the target tumor spheroids that confirmed the creatable role of folate receptors.

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Preparation and Evaluation of the Antibacterial Effect of Magnetic Nanoparticles Containing Gentamicin: A Preliminary In vitro Study

Cited by 11 publications

References 25 publications

Design and Development of Antibacterial/Anti-inflammatory Dual Drug-Loaded Nanofibrous Inserts for Ophthalmic Sustained Delivery of Gentamicin and Methylprednisolone: In Vitro Bioassay, Solvent, and Method Effects’ Evaluation

Design and Development of Antibacterial/Anti-inflammatory Dual Drug-Loaded Nanofibrous Inserts for Ophthalmic Sustained Delivery of Gentamicin and Methylprednisolone: In Vitro Bioassay, Solvent, and Method Effects’ Evaluation

Modification of nonwoven polymer materials for increasing of their filtration and antibacterial properties

Cytotoxicity Effects of Curcumin Loaded on Chitosan Alginate Nanospheres on the KMBC-10 Spheroids Cell Line

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