Preparation and evaluation of molecularly imprinted microspheres for solid‐phase extraction of 1,4‐hydroxybenzoic acid esters in soy

Abstract

Molecularly imprinted microspheres (MIMs) were prepared by precipitation polymerization for the binding and recognition of 1,4-hydroxybenzoic acid esters. Ethyl p-hydroxybenzoate (EtPHB) was used as the template molecule, methacrylic acid as the functional monomer, ethylene dimethacrylate as the linking agent. It was evaluated by solid-phase extraction column packed with MIMs combined with liquid chromatography to determine trace preservatives including benzoic acid, methyl p-hydroxybenzoate, EtPHB, propyl p-hydroxybenzoate in food products. A solid-phase extraction based on MIM procedure was used to isolate four additives from the food matrix before quantitative analysis. The Scatchard plot suggested that the template-polymer system had two-site binding behavior with the dissociation constants of 0.3577 and 3.952 mg/g, respectively. The rebinding test, based on the molecularly imprinted solid-phase extraction column technique, showed the recoveries of soy samples spiked with four additives within 88.4-110.6%, with the relative standard deviations of 1.97-3.82%. Finally, the method was successfully applied for the analysis of parabens in foodstuff without traditional pretreatment.

Preparation and selective recognition of a novel solid‐phase microextraction fiber combined with molecularly imprinted polymers for the extraction of parabens in soy sample

“…Molecularly imprinting technology is a molecular recognition technology and molecularly imprinted polymers (MIPs), with high selectivity, have shown potential applications as SPE sorbent [12][13][14] and SPME fiber coating to recognize the template and other closely related compounds [15]. The use of MIP-coated SPME (MI-SPME) [16,17], instead of other pretreatment methods, is expected to reduce assay time, as well as the cost of solvents and disposal and increase the selectivity of the determinations for complex matrices.…”

Synthesis of Magnetic Molecularly Imprinted Polymer Sorbents for Isolation of Parabens from Breast Milk

“…As can be seen from the results collected in Table 3 , only one text was found, which described the use of a polymer with an imprinted particle with magnetic properties [ 26 ]. In most cases, sample preparation was based on the use of the obtained sorbent in the SPE [ 27 , 29 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ], which requires packing an SPE cartridge. In our opinion, the use of the sorbent in bulk form as a dispersive solid-phase extraction allows a quicker sample preparation.…”

Application of Molecularly Imprinted Polymers in the Analysis of Waters and Wastewaters

“…The application of MIP sorbents allows not only preconcentration and cleaning of the sample, but also selective extraction of the target analyte, using a very small amount of imprinted polymer (typically 5 to 200 mg) packed into a cartridge. MISPE is an efficient approach for the isolation and preconcentration of analytes from complex matrices and was successfully reported for environmental waters [38], soils [39], sediments [40], plant extracts [41], and soy [42].…”