Preparation and evaluation of liposomal formulations of tropicamide for ocular delivery

Nagarsenker, Mangal S.; Londhe, Vaishali; Nadkarni, G.D.

doi:10.1016/s0378-5173(99)00265-3

Cited by 116 publications

(56 citation statements)

References 8 publications

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“…To fix this trouble, a number of efforts were performed in the former times by formulating miscellaneous ophthalmic drug delivery systems in disparate formats such as hydrogels (Derwent and Mieler 2008), microparticles (Joshi 1994), nanoparticles (Nagarwal et al 2009), liposomes (Nagarsenker et al 1999), collagen shields (Yoel and Guy 2008), ocular inserts/discs (Bloomfield et al 1978), dendrimers (Vandamme and Brobeck 2005) and transcorneal iontophoresis (Monti et al 2003) etc. All of the attempts made in the history were executed with a target to defeat the swift elimination of active agent from the precorneal cavity of the eye and to boost the corneal residence time of the drug molecules.…”

Section: Introductionmentioning

confidence: 99%

In vitro, in vivo and pharmacokinetic assessment of amikacin sulphate laden polymeric nanoparticles meant for controlled ocular drug delivery

et al. 2014

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The rationale of current exploration was to formulate positively charged amikacin-loaded polymeric nanoparticles providing a controlled release attribute. Amikacin sulphate-loaded nanoparticles were prepared by w/o/w emulsification solvent evaporation approach succeeded by high-pressure homogenization. Two bioadhesive positively charged polymers, Eudragit Ò RS 100 and Eudragit Ò RL 100, were used in the blend, with variable ratios of drug and polymer. The formulations were assessed in terms of particle size and zeta potential. Thermal gravimetric analysis was brought out on the samples of drug, polymer and drug polymer complex. Drug loading and release attributes of the nanoparticles were scrutinized and antimicrobial activity in contrast to Staphylococcus aureus was appraised. Ocular irritation test, in vivo ocular retention study, in vivo release profile (permeation study) and in vivo antibacterial activity of polymeric nanosuspensions were executed. No rupture consequence but a lengthened drug release was contemplated from all formulations. Amikacin sulphate release from the polymeric nanoparticles reflected a better fit with Korsmeyer-Peppas model. In the course of the antibacterial activity of nanoparticles against S. aureus, formulation AE1 displays the most prominent inhibitory effect as compared with marketed formulation of amikacin sulphate.

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Section: Introductionmentioning

confidence: 99%

In vitro, in vivo and pharmacokinetic assessment of amikacin sulphate laden polymeric nanoparticles meant for controlled ocular drug delivery

et al. 2014

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“…Successful results have been obtained with inserts (3,4), collagen shields (5), temperature sensitive in situ-forming gel (6)(7)(8), liposome (9)(10)(11), and microsphere and nanosphere (12,13). However, taking into consideration of patients' compliance, manufacturing cost, dosage adherence, and blurring effect these dosage forms may have, a simple aqueous solution with moderate viscosity is still preferred.…”

Section: Introductionmentioning

confidence: 99%

Development of an Ex Vivo Method for Evaluation of Precorneal Residence of Topical Ophthalmic Formulations

Liu

Wang

2009

AAPS PharmSciTech

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Abstract. This paper describes the development of an ex vivo perfusion method for the evaluation of topical ophthalmic formulations. The perfusion system developed consisted of a perfusion chamber, two precision pumps to control the in/out flow rates to simulate the tear flow rate, and a fluorescence microscope imager. Freshly excised rat cornea was used as a biomembrane. Fluorescein (FITC) was used as a marker. Residence time was determined by measuring fluorescence intensity over time after application of the formulation to the cornea. In addition, viscoelastic properties of the formulations were measured and correlated to the retention times. The perfusion method easily differentiated formulations based on the retention time on the cornea: For example, a 0.3% hydroxypropyl methylcellulose formulation had a short retention time of <10 min. Addition of 0.25% carboxymethylcellulose increased the retention time from less than 10 min to over 16 min, and addition of 0.1% Carbopol further increased retention time to over 40 min. For alginate formulations, the retention time was significantly longer in the presence of 0.06% calcium chloride than that of 0.006% calcium chloride. The longer residence time at a higher Ca++ concentration can be attributed to the greater elastic modulus associated with the gel. Interestingly, however, a hyaluronate formulation displayed a very long retention time but has low viscoelastic moduli. This suggests that the mucoadhesive properties may not always be discernable by the rheological properties. The ex vivo perfusion method may in fact provide more meaningful information with regard to retention times of formulations.

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“…After 3 min, second cycle was processed for 3 min at 80 % amplitude with 0.5 sec pulse for another 3 min. 10,11 After sonication the heterogeneous liposomal suspension of SUVs (small uni-lamellar vesicles) were converted to homogenous suspension of SUVs by passing through 0.22 μ syringe filter which further improves the polydispersibility index and also achieved the sterilization of the liposomal suspension which can be administered by intravenous route. Liposomal formulations prepared by film hydration method were coded as LF1, LF2, LF3, LF4, LF5, LF6, and LF7.…”

Section: Amentioning

confidence: 99%

Development and Investigation of Timolol Maleate and Latanoprost Combination Liposomes for the Treatment of Glaucoma

Dubey¹,

Prabhu²,

Beladiya³

et al. 2015

Int. Res. J. Pharm.

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In this study liposomes were developed and evaluated for timolol maleate (TM) and latanoprost (LP) by lipid film hydration technique for the treatment of glaucoma. A 3 2 factorial design was utilized to study the effect of the molar ratio of 1, 2 -Dipalmitoyl-sn-glycero-3-phosphocholine (DPCC) (X1) and cholesterol (X2) on vesicle size, drug entrapment efficiency and in-vitro release study. Fourier transform infrared spectroscopy (FT-IR) studies were performed to investigate and predict any physiological interactions between components in the formulation. The liposomal vesicles were found to be uniform in size and shape. The drug entrapment efficiency values for the seven batches showed a variation from a minimum of 56.65 ± 1.39 % to maximum of 70.24 ± 1.14 %. This clearly indicates that the drug entrapment value is strongly dependent on the variables selected. The Zeta potential, average particle size and poly dispersibility index was found to be -17.3 mV, 240 nm and 0.114 respectively. Most of the formulations were found to have a linear release and the formulations were found to provide approximately 72 % -55 % of drug release for TM and LP respectively within a period of 6 h. Kinetic study reveals that release mechanism depends on swelling, diffusion and relaxation. Best formulation (F2) was found to be sterile and stable and free from microorganisms and passes the isotonicity test. Reduction in intra ocular pressure (IOP) was greater in liposomes as compared to the marketed product of alone and combination medication. A latanoprost-timolol maleate fixed combination, has the advantage of a more convenient delivery system as well as providing a simple summing effect of the two constituents.

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Preparation and evaluation of liposomal formulations of tropicamide for ocular delivery

Cited by 116 publications

References 8 publications

In vitro, in vivo and pharmacokinetic assessment of amikacin sulphate laden polymeric nanoparticles meant for controlled ocular drug delivery

In vitro, in vivo and pharmacokinetic assessment of amikacin sulphate laden polymeric nanoparticles meant for controlled ocular drug delivery

Development of an Ex Vivo Method for Evaluation of Precorneal Residence of Topical Ophthalmic Formulations

Development and Investigation of Timolol Maleate and Latanoprost Combination Liposomes for the Treatment of Glaucoma

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