Preparation and evaluation of chitosan-gelatin composite scaffolds modified with chondroitin-6-sulphate

Abstract

The objective of the present study was to investigate the properties of porous chitosan–gelatin scaffolds. The modification of samples was done by incorporation of chondroitin-6-sulphate in bulk phase through cross linking with glutaraldehyde. Scaffolds were prepared using the freeze-drying method. An in vitro cell culture of fibroblasts L-929 was employed to study the growth and attachment of cells to the scaffolds. Optical microscopy showed high cell density for the sample supplemented with chondroitin-6-sulphate. Scanning electron microscopy was used to analyze the growth and attachment of cells. Results of the tests revealed that the sample with a 65: 35 ratio of gelatin and chitosan was suitable for both cell attachment and proliferation in fibroblast cultures. The cell viability 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay with human mesenchymal stem cells on the previous sample showed about 80 % increase in cell viability. To analyze the mechanical properties of scaffolds, the tensile strengths of the samples were measured and all samples showed stress at break above 0.1 MPa. Optimum substratum composition for porous scaffolds would involve a compromise between the desired attributes of biocompatibility, promotion of cell growth and mechanical strength.

Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering

“…25,26 Chitosan has been widely used in this field as well. 27,28 However, most of the previous studies focus on a single polymer for fabrication of electrospun nanofibrous scaffolds.…”

Cartilage and facial muscle tissue engineering and regeneration: a mini review

“…In regard to tissue engineering of a particular cartilage, although implanting artificial matrices, growth factors, perichondrium, and periosteum can initiate the formation of cartilaginous tissue in osteochondral and chondral defects in synovial fluids, the results vary considerably from patient to patient [16]. A successful scaffold is one that fits the anatomical defects defined from clinical imaging data, while also having a design that is porous and that can balance load bearing and biofactor delivery requirements [17][18][19][20][21][22][23][24][25][26][27][28][29][30]. For these designs, the global image design database is created from a computed tomography (CT) or magnetic resonance (MR) image of a patient.…”

Gelatin/chondroitin sulfate nanofibrous scaffolds for stimulation of wound healing: In‐vitro and in‐vivo study

“…Chondroitin sulphate has also an effective role in wound healing and tissue regeneration. The multiple biological functions of GAGs suggest that, as biomaterials, they have a great potential for tissue engineering and wound healing . Therefore, it would be a great idea to mix it with gelatin for fabrication of proper nanofibrous scaffold to be used as artificial ECM in tissue engineering .…”

“…The multiple biological functions of GAGs suggest that, as biomaterials, they have a great potential for tissue engineering and wound healing. [18][19][20][21] Therefore, it would be a great idea to mix it with gelatin for fabrication of proper nanofibrous scaffold to be used as artificial ECM in tissue engineering. 17,22 In this study, we adopted electrospinning to fabricate nanofibrous scaffolds based on gelatin and chondroitin sulfate (GAG) in trifluoroethanol (TFE) and water as a solvent system.…”