Preparation and evaluation of biopolymeric nanoparticles as drug delivery system in effective treatment of rheumatoid arthritis

Kumar, Vijay; Leekha, Ankita; Tyagi, A. K.; Kaul, Ankur; Mishra, Anil K.; Verma, Anita

doi:10.1007/s11095-016-2094-y

Cited by 56 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding hemocompatibility assessment, our studies also allowed to clarify some conflicting literature results. Considering the hemolytic activity, some original articles were found supporting the non-hemolytic activity of Chit NPs (Nadesh et al, 2013;Kumar et al, 2017) and also the Chit polymer (Nadesh et al, 2013). Nevertheless, two studies reported a slight hemolytic effect for Chit NPs (Shelma and Sharma, 2011;de Lima et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Chitosan Nanoparticles: Shedding Light on Immunotoxicity and Hemocompatibility

Jesus

Marques

Duarte

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Nanoparticles (NPs) assumed an important role in the area of drug delivery. Despite the number of studies including NPs are growing over the last years, their side effects on the immune system are often ignored or omitted. One of the most studied polymers in the nano based drug delivery system field is chitosan (Chit). In the scientific literature, although the physicochemical properties [molecular weight (MW) or deacetylation degree (DDA)] of the chitosan, endotoxin contamination and appropriate testing controls are rarely reported, they can strongly influence immunotoxicity results. The present work aimed to study the immunotoxicity of NPs produced with different DDA and MW Chit polymers and to benchmark it against the polymer itself. Chit NPs were prepared based on the ionic gelation of Chit with sodium tripolyphosphate (TPP). This method allowed the production of two different NPs: Chit 80% NPs (80% DDA) and Chit 93% NPs (93% DDA). In general, we found greater reduction in cell viability induced by Chit NPs than the respective Chit polymers when tested in vitro using human peripheral blood monocytes (PBMCs) or RAW 264.7 cell line. In addition, Chit 80% NPs were more cytotoxic for PBMCs, increased reactive oxygen species (ROS) production (above 156 µg/mL) in the RAW 264.7 cell line and interfered with the intrinsic pathway of coagulation (at 1 mg/mL) when compared to Chit 93% NPs. On the other hand, only Chit 93% NPs induced platelet aggregation (at 2 mg/mL). Although Chit NPs and Chit polymers did not stimulate the nitric oxide (NO) production in RAW 264.7 cells, they induced a decrease in lipopolysaccharide (LPS)-induced NO production at all tested concentrations. None of Chit NPs and polymers caused hemolysis, nor induced PBMCs to secrete TNF-α and IL-6 cytokines. From the obtained results we concluded that the DDA of the Chit polymer and the size of Chit NPs influence the in vitro immunotoxicity results. As the NPs are more cytotoxic than the corresponding polymers, one should be careful in the extrapolation of trends from the polymer to the NPs, and in the comparisons among delivery systems prepared with different DDA chitosans.

show abstract

Section: Discussionmentioning

confidence: 99%

Chitosan Nanoparticles: Shedding Light on Immunotoxicity and Hemocompatibility

Jesus

Marques

Duarte

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…[49] Moreover, no burst release effect was present, which is a very common and problematic disadvantage of many reported NPs in literature, due to weak electrostatic interactions, such as is the case of polymeric micro-and NPs. [50][51][52][53][54] The Dex cumulative release percentage has slightly increased over time, reaching ca. 5% after 7 h and ca.…”

Section: Preparation and Functionalization Of Melanin Nanoparticlesmentioning

confidence: 99%

Electro-responsive controlled drug delivery from melanin nanoparticles

Caldas

Santos

Rebelo

et al. 2020

International Journal of Pharmaceutics

View full text Add to dashboard Cite

A n a Cl á u d i a S a n t o s , a p r e s e n t a d o s à F a c u l d a d e d e F a r má c i a d a Un i v e r s i d a d e d e Co i mb r a , p a r a a p r e c i a ç ã o n a p r e s t a ç ã o d e p r o v a s p ú b l i c a s d e Me s t r a d o I n t e g r a d o e m Ci ê n c i a s F a r ma c ê u t i c a s. s e t e mb r o 2 0 1 8 Eu, Mariana Elisabete Rodrigues Caldas, estudante do Mestrado Integrado em Ciências Farmacêuticas, com o nº 2012133041, declaro assumir toda a responsabilidade pelo conteúdo do Documento Relatório de Estágio e Monografia intitulada "Electro-responsive controlled drug delivery from melanin nanoparticles" apresentados à Faculdade de Farmácia da Universidade de Coimbra, no âmbito da unidade curricular de Estágio Curricular. Mais declaro que este Documento é um trabalho original e que toda e qualquer afirmação ou expressão, por mim utilizada, está referenciada na Bibliografia, segundo os critérios bibliográficos legalmente estabelecidos, salvaguardando sempre os Direitos de Autor, à exceção das minhas opiniões pessoais. Coimbra, 07 de setembro de 2018 (Mariana Elisabete Rodrigues Caldas) Palavras-chave: melanina; biopolímeros; material natural; nanopartícula; libertação de fármaco; libertação de fármaco sensível a estímulos elétricos.

show abstract

“…The chitosan nanoparticle (CHNP) loaded with dexamethasone (DEX)/(MTX) was developed by Kumar et al [48] for the efficient treatment of RA. They prepared chitosan nanoparticle using ionic gelation method.…”

Section: Nanoparticles For the Treatment Of Ramentioning

confidence: 99%

Novel nano therapeutic materials for the effective treatment of rheumatoid arthritis-recent insights

Janakiraman

Krishnaswami

Rajendran

et al. 2018

Materials Today Communications

View full text Add to dashboard Cite

A B S T R A C TRheumatoid arthritis (RA) is the most common complex multifactorial joint related autoimmune inflammatory disease with unknown etiology accomplished with increased cardiovascular risks. RA is characterized by the clinical findings of synovial inflammation, autoantibody production, and cartilage/bone destruction, cardiovascular, pulmonary and skeletal disorders. Pro-inflammatory cytokines such as IL-1, IL-6, IL-8, and IL-10 were responsible for the induction of inflammation in RA patients. Drawbacks such as poor efficacy, higher doses, frequent administration, low responsiveness, and higher cost and serious side effects were associated with the conventional dosage forms for RA treatment. Nanomedicines were recently gaining more interest towards the treatment of RA, and researchers were also focusing towards the development of various anti-inflammatory drug loaded nanoformulations with an aid to both actively/passively targeting the inflamed site to afford an effective treatment regimen for RA. Alterations in the surface area and nanoscale size of the nanoformulations elicit beneficial physical and chemical properties for better pharmacological activities. These drug loaded nanoformulations may enhances the solubility of poorly water soluble drugs, improves the bioavailability, affords targetability and may improve the therapeutic activity. In this regimen, the present review focus towards the novel nanoparticulate formulations (nanoparticles, nanoemulsions, solid lipid nanoparticles, nanomicelles, and nanocapsules) utilized for the treatment of RA. The recent advancements such as siRNA, peptide and targeted based nanoparticulate systems for RA treatment were also discussed. Special emphasis was provided regarding the pathophysiology, prevalence and symptoms towards the development of RA.

show abstract

Preparation and evaluation of biopolymeric nanoparticles as drug delivery system in effective treatment of rheumatoid arthritis

Abstract: The nanocrystalline biopolymeric nanoparticles were stable, biocompatible and have potential to be administered through i.p. route with minimal toxicity and high efficacy.

Cited by 56 publications

References 32 publications

Chitosan Nanoparticles: Shedding Light on Immunotoxicity and Hemocompatibility

Chitosan Nanoparticles: Shedding Light on Immunotoxicity and Hemocompatibility

Electro-responsive controlled drug delivery from melanin nanoparticles

Novel nano therapeutic materials for the effective treatment of rheumatoid arthritis-recent insights

Contact Info

Product

Resources

About