Preparation and evaluation of a new biopesticide solution candidate for plant disease control using pexiganan gene and Pichia pastoris expression system

Neshani, Alireza; Tanhaeian, Abbas; Zare, Hosna; Eidgahi, Mohammad Reza Akbari; Ghazvini, Kiarash

doi:10.1016/j.genrep.2019.100509

Cited by 10 publications

(6 citation statements)

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“…Cloning, expression and purification of recombinant Pexiganan was done as previously described [15]. Briefly, the pGH vector containing Pexiganan optimized sequence was transformed into Escherichia coli TOP10 according to efficient expression in Pichia pastoris.…”

Section: Cloning and Expression Of Pexigananmentioning

confidence: 99%

Preparation and evaluation of antibacterial properties of Pexiganan, a Magainin analogue with Broadly-Spectrum Antimicrobial Activity

Ghazvini¹,

Neshani²,

Farsiani³

2021

PJMHS

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Introduction: Pexiganan is one of the most popular Maganin-2 analogues, which has broadly- spectrum bactericidal effects against both gram-positive and gram-negative bacteria. In this study, the effects of temperature, pH, and in vitro resistant analysis were evaluated on the antibacterial activity of this peptide. Materials and Methods: Pexiganan recombinant was expressed in P. pastoris GS115, and after its characterization, the cell cytotoxicity characteristics, effects of temperature on antibacterial activity, as well as the emergence of resistance to pexiganan in continuous sub-cultures and MIC of this peptide were investigated against 12 bacterial pathogens. Results: Pexiganan was not cytotoxic to HeLa cell lines. Increasing the temperature had no effect on the zone of inhibition, but increasing the pH caused a significant increase in MIC against S. aureus ATCC 29213 strain. In addition, Pexiganan had inhibitory properties against all the 12 human pathogens (MIC range: 1-32 /g / ml), and no resistance was observed even after 28 sub- cultures. Conclusion: Pexiganan is an AMP with antimicrobial properties against a wide range of susceptible and drug-resistant human pathogens with a very low rate of resistance compared to available antibiotics and is a reliable candidate for further investigation on therapeutic applications. Keywords: Pexiganan; Antimicrobial Peptides; Resistance; Minimum Inhibitory Concentration

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Section: Cloning and Expression Of Pexigananmentioning

confidence: 99%

Preparation and evaluation of antibacterial properties of Pexiganan, a Magainin analogue with Broadly-Spectrum Antimicrobial Activity

Ghazvini¹,

Neshani²,

Farsiani³

2021

PJMHS

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“…Peptide has high long-term stability and temperature resistance. According to calculations, production of 52 mg of recombinant peptide costs about 10 $, while synthetic production of crude pexiganan is 20-30 times more expensive and in case of 98 % purity, it is even 120 times more expensive (Neshani et al, 2019), (www.entonbio. com; www.synpeptide.com).…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

“…1. Minimum inhibitory concentration of recombinant pexiganan and cooper oxychloride needed for lethal effect on pathogen (Neshani et al, 2019). detected in soil and drinking water long after their application and they belong to the most toxic pesticides causing long-term damage to human health since they inhibit the enzyme acetylcholinesterase which is essential for the function of central nervous system (Songa & Okonkwo, 2016).…”

Section: Pesticidesmentioning

confidence: 99%

“…Pexiganan replacement of pesticides Neshani et al, 2019 Organophosphorus hydrolase biodegradation of organophosphorus pesticides Shen et al, 2016 Kpkt β-1,3glucanase and killer activity against spoilage yeast in wine and sweet beverage industry Chessa et al, 2017 Laccase degradation of synthetic dyes, lignin, nitrophenol, antibiotics and phenolic pollutants Zhou et al, 2018;Colao et al, 2006 Manganese peroxidase degradation of lignin, cellulose, hemicellulose, polycyclic aromatic hydrocarbons, chlorophenols, synthetic dyes, nitroaromatics, tetracyclines, oxytetracyclines, bleaching of pulp Xu et al, 2017;Xu et al, 2010 Lignin peroxidase degradation of lignin Wei & Xianghua, 2009 Xylanase conversion of lignocellulosic material and agrowaste to high-value products, paper pulping and bleaching Ma et al, 2019 Phytase helps to digest phytate which prevent phosphorus pollution Tang et al, 2009 Nitrate reductase test nitrates in water www.nitrate.com…”

Section: Product Environmental Treatment Referencementioning

confidence: 99%

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Pichia pastoris — recombinant enzyme producent for environment treatment — review

Kyzeková

Krasňan

Rebroš

2020

Acta Chimica Slovaca

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Since environmental pollution is increasing, scientists try to find a sustainable way for its clean up and for environment protection. Due to increasing knowledge of genetics and recombinant technologies, recombinant enzymes have been increasingly applied for these purposes. This article deals with the possibilities of environmental treatment with different types of enzymes produced by P. pastoris. Environment is polluted mostly with pesticides, wastewaters, phenol compounds, plastics, toxic compounds, wastes from medical treatment, etc. All these compounds have to be eliminated considering the deteriorating biodiversity, human health, and condition of plants. Enzymes are an environmentally friendly way of such treatment.

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“…4,11 The indiscriminate use of synthetic herbicides and the lack of products with a specific mode of action favor the emergence of resistant weeds and reduce the effectiveness of these products, increasing production costs and reducing crop productivity. 12 Thus, bioherbicides produced with metabolites like α-bisabolol emerge as a more environmentally safe and efficient alternative in the management of weeds. 13,14 Thus, it is hypothesized that the essential oil of V. arborea (EOVA) and α-bisabolol (the major component of EOVA) inhibit seed germination and reduce the growth of weed species by inducing oxidative stress and affecting photosynthesis, while in nontarget species (crops), there is no damage.…”

Section: Introductionmentioning

confidence: 99%

New Perspective on the Use of α-Bisabolol for Weed Control

Chaves,

Dias,

Pereira

et al. 2024

J. Agric. Food Chem.

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The indiscriminate use of synthetic herbicides reduces its effectiveness. Bioherbicides produced with metabolites emerge as an alternative to managing weeds. We aimed to analyze the phytotoxic potential of the essential oil of Vanillosmopsis arborea (EOVA) and the α-bisabolol molecule, its main component. We evaluated the effects of EOVA and α-bisabolol at different concentrations on the germination, growth, antioxidant metabolism, and photosynthesis of different species. EOVA and α-bisabolol showed promising phytotoxic effects on the germination and initial growth of the weed Senna occidentalis, inhibiting the activity of the antioxidant enzymes and increasing lipid peroxidation. α-Bisabolol reduced the weed seedling growth by inducing oxidative stress, which suggests a greater role in postemergence. Moreover, in the weed postemergence, both EOVA and α-bisabolol caused damage in the shoots, reduced the chlorophyll content, and increased lipid peroxidation besides reducing photosynthesis in S. occidentalis. Overall, we suggest the promising action of α-bisabolol and EOVA as bioherbicides for weed control.

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Preparation and evaluation of a new biopesticide solution candidate for plant disease control using pexiganan gene and Pichia pastoris expression system

Cited by 10 publications

References 42 publications

Preparation and evaluation of antibacterial properties of Pexiganan, a Magainin analogue with Broadly-Spectrum Antimicrobial Activity

Preparation and evaluation of antibacterial properties of Pexiganan, a Magainin analogue with Broadly-Spectrum Antimicrobial Activity

Pichia pastoris — recombinant enzyme producent for environment treatment — review

New Perspective on the Use of α-Bisabolol for Weed Control

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