While the effect of fertilizing treatment on vitamin content of plants is known, relatively little information exists concerning the influence of trace elements. I n an early report, Honeywell and Dutcher (1930) stated that chlorotic spinach grown on soils deficient in manganese contained less vitamin A than normal spinach. Recently Lo and W u (1943) found that the compounds of nickel, molybdenum, aluminum, zinc, copper, and iron so far studied have a physiological function of promoting vitamin P formation in mung-bean sprouts. Since it was not known whether the other parts of plants were similarly influenced, the present investigation, dealing with the effect of specific chemical treatment on the carotene and citrin contents of pea shoots and green peas by field experiment, was initiated. This seemed to be a worthy problem from both biochemical and practical standpoints.
EXPERIMENTAL PROCEDUREOne set of field experiments was laid out to test the effect of some specific chemicals (sulphates of nickel, aluminum, and zinc as well as molybdenum oxide) and a complete fertilizer (mixture of ammonium sulphate, bone meal, and straw-ash), singly and in combination. A native variety of pea (Pisum sativum, L . ) , namely Meitan Ying-Chia-Wan-Tou, was used as the experimental plant. The field experiment consisted of three blocks of plots laid out in randomized fashion, giving 10 treatments with three replicates. All plots were three and one-half by five feet with two rows of plants per plot. Each plot had six individual plants. The outside rows of each plot served as guard rows, preventing contamination of any plot by the chemicals and fertilizer used on adjacent plots. Application of the complete fertilizer (ammonium sulphate : bone meal : strawash = 3:1:4 by weight) was roughly such as would be 64 catties per mou before the plant seedlings were planted. All the specific chemicals were used in a concentration of 0.0001 N and applied in 50-c.e. portions per plant at 10-day intervals during the entire experimental period.The method of carotene determination was that recommended by Hughes and Peterson, as reported by Munsey (1937), and the citrin was estimated by a modification of the Lorenz and Arnold (1941) method described in previous papers. Six determinations were made in all instances; results are given (Tables 1 2, 3, and 4).
DISCUSSION AND CONCLUSIONFrom an inspection of the above tables it is evident that there was a considerable variation in the carotene and citrin contents of pea shoots 308