Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen

Komiyama, Nobuko; Hiemori, Miki; Kimoto, Masumi; Suzuki, Makiko; Yamashita, Hiromi; Takahashi, Yoshitaka; Tsuji, Hideaki

doi:10.1271/bbb.90224

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Allergens In Wheat1

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“…a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, which it recognized the allergen purified from wheat flour, and the epitope on the allergen to mAb was determined to be amino acid sequence 154VPWVVVDGKPL164 of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163 [21].…”

Section: Allergens In Wheatmentioning

confidence: 99%

Allergens in Wheat: Review

Feng

Zhang

Chen

et al. 2012

AMR

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Wheat is the most important foodstuff of the cereals in view of its yield and consumption quantity in the world. However, allergens in wheat become one of the most severe medical problems. Therefore, recent research progresses on allergens of wheat were reviewed in this paper. Some allergens in wheat, for example, wheat lipid transfer protein, ω-5 gliadin, and α-amylase inhibit, have been analysed clearly, whereas others, salt-soluble protein, hydrolysed wheat proteins, wheat-germ agglutinin, and polysaccharide, were an obscure explanation.

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Section: Allergens In Wheatmentioning

confidence: 99%

Allergens in Wheat: Review

Feng

Zhang

Chen

et al. 2012

AMR

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Investigation of Multiple Forms of Tri a Bd 27K, a Major Wheat Allergen, by Immunoblotting Analysis

Hiemori

Yosida

Kimoto

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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Tri a Bd 27K, a major wheat allergen, is a glycoprotein. Tri a Bd 27K was found to occur in multiple forms by two-dimensional polyacrylamide gel electrophoresis and immunoblotting with a monoclonal antibody against the allergen. Furthermore, it was found that only Tri a Bd 27K components, which have N-linked glycan moieties with fucose residues, bound to IgE antibodies in the sera of wheat-sensitive patients.

show abstract

Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen

Cited by 2 publications

References 10 publications

Allergens in Wheat: Review

Allergens in Wheat: Review

Investigation of Multiple Forms of Tri a Bd 27K, a Major Wheat Allergen, by Immunoblotting Analysis

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