Preparation and Diagnostic Value of an Antiserum for Placental Protein

Cohen, Harold H.; Freda, V.

doi:10.3181/00379727-43-11080p

Cited by 5 publications

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“…In a few instances particular protein fractions have been tested. By serological methods, more or less strict organ specificity has been shown for brain (6,7), testicle (7), kidney (8)(9)(10), suprarenal gland (11), placenta (12,13), epiphysis (14), hypophysis (15), stomach and intestine (16), lens and intestine (17), leucocytes (18), carcinoma and sarcoma (19)(20)(21)(22), and glioma (23). With some organs, the specificity of antisera has been shown in vivo, either directly by the action of cytotoxins, as in the case of the injection of anti-kidney sera for the production of nephritis (24,25), or indirectly by the effects on metabolism induced by the injection of antisera for thyroid gland and the anterior body of the pituitary gland (26).…”

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Organ Specificity of Tissues of the Dog and Man as Shown by Passive Anaphylaxis in Guinea Pigs

Bailey

Raffel

1941

Journal of Experimental Medicine

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In previous studies (1, 2) it was shown that prolonged immunization of rabbits with sedimented bacterial vaccines grown in infusion broths prepared from the tissues of different animals, stimulated the production of antibodies not only for the bacteria but also for the broths. It was demonstrated that fatal anaphylaxis usually followed the injection of homologous organ broths into guinea pigs passively sensitized with the antisera of rabbits immunized in this way. Thus, almost complete immunological specificity for broths made from striated muscle and brain was so obtained. It was found that some of the organ-specific substances still remained in infusions of these tissues after they had been autoclaved. The partial destruction or conjugation of the relatively small amounts of blood in the infusions by such treatment, seemed to provide a simple method which gave promise of possible application to various tissues in an effort to demonstrate organ specificity by the elimination of the numerous cross-reactions which are usually obtained in immunological tests with suspensions and extracts of unheated cells.Reviews of the voluminous literature on recent studies of organ specificity carried out by various methods, have been made by Witebsky (3), Landsteiner (4), and others, to which the reader is referred. It should be stated, however, that most of these investigations have been made by means of either the complement-fixation or the precipitin reaction with alcoholic extracts of fresh organs. Sometimes aqueous solutions or suspensions of fresh tissues were used as antigens (5). In a few instances particular protein fractions have been tested. By serological methods, more or less strict organ specificity has been shown for brain (6, 7), testicle (7), kidney (8-10), suprarenal gland (11), placenta (12, 13), epiphysis (14), hypophysis (15), stomach and intestine (16), lens and intestine (17), leucocytes (18), carcinoma and sarcoma (19)(20)(21)(22), and glioma (23). With some organs, the specificity of antisera has been shown in vivo, either directly by the action of cytotoxins, as in the case of the injection of anti-kidney sera for the production of nephritis (24, 25), or indirectly by the effects on metabolism induced by the injection of antisera for thyroid gland and the anterior body of the pituitary gland (26).

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