Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle

Chevalier, Clément; Nicolas, Jean‐François; Petit, Anne-Cécile

doi:10.1007/7651_2013_63

Cited by 18 publications

(10 citation statements)

References 11 publications

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“…To activate CreERT2 in pups, we administered 120 µg/g body weight of tamoxifen (T5648) or 20 µg/g of 4-OH tamoxifen (H6278) diluted in a 1:1:2 solution of EtOH:Cremophor:phosphate buffered saline (PBS) 56 . To investigate the function of p21 in angiogenesis, we used Cdkn1a (p21) knockout mice 57 .…”

Section: Methodsmentioning

confidence: 99%

High mitogenic stimulation arrests angiogenesis

et al. 2019

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Appropriate therapeutic modulation of endothelial proliferation and sprouting is essential for the effective inhibition of angiogenesis in cancer or its induction in cardiovascular disease. The current view is that an increase in growth factor concentration, and the resulting mitogenic activity, increases both endothelial proliferation and sprouting. Here, we modulate mitogenic stimuli in different vascular contexts by interfering with the function of the VEGF and Notch signalling pathways at high spatiotemporal resolution in vivo. Contrary to the prevailing view, our results indicate that high mitogenic stimulation induced by VEGF, or Notch inhibition, arrests the proliferation of angiogenic vessels. This is due to the existence of a bell-shaped dose-response to VEGF and MAPK activity that is counteracted by Notch and p21, determining whether endothelial cells sprout, proliferate, or become quiescent. The identified mechanism should be considered to achieve optimal therapeutic modulation of angiogenesis.

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Section: Methodsmentioning

confidence: 99%

High mitogenic stimulation arrests angiogenesis

et al. 2019

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“…Conditional induction of Cherry-VANGL2 protein was performed by intraperitoneal injection of 4-OH tamoxifen (4-OHTm, Sigma: H6278) at a concentration of 66.6 mg/g in Rosa26CreER females mated with Cherry-Vangl2 males. The 4-OHTm solution was prepared as described previously (Chevalier et al, 2014). For embryo staging, the day of vaginal plug was considered E0.5.,All the embryonic stages used are stated in the main text or in the figure legends.…”

Section: Resource Availabilitymentioning

confidence: 99%

Apical Restriction of the Planar Cell Polarity Component VANGL in Pancreatic Ducts Is Required to Maintain Epithelial Integrity

et al. 2020

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“…Conditional induction of Cherry-VANGL2 protein was performed by intraperitoneal injection of 4-OH tamoxifen (4-OHTm, Sigma, H6278) at a concentration of 66,6 μg/g in Rosa26CreER females mated with Cherry-Vangl2 males. The 4-OHTm solution was prepared following instructions described in Chevalier et al 80.…”

Section: Methodsmentioning

confidence: 99%

Apical restriction of the planar cell polarity component VANGL in pancreatic ducts is required to maintain epithelial integrity

Flasse

Yennek

Cortijo

et al. 2019

Preprint

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15Cell polarity is essential for the architecture and function of numerous epithelial tissues. Here we show 16 how planar cell polarity (PCP), so far studied principally in flat epithelia, is deployed during the 17 morphogenesis of a tubular organ. Using the mammalian pancreas as a model, we report that 18 components of the core PCP pathway such as the transmembrane protein Van Gogh-like (VANGL), are 19 progressively apically-restricted. VANGL expression becomes asymmetrically localized at the apical 20 surface of ductal cells, revealing a planar polarization of the pancreatic duct. We further show that 21 restricting VANGL to these discrete sites of expression is crucial for epithelial integrity. Expansion of 22 expression on basolateral membranes of the progenitors leads to their death and extrusion from the 23 epithelium, as previously observed for perturbations of apico-basal polarity. Using organoids and in vivo 24 analyses, we show that cell elimination is induced by a decrease of Rock activity via Dishevelled. 25 2 group of six families of proteins is required to coordinate planar polarization between neighboring cells: 38 the transmembrane proteins Frizzled (vertebrate: FZD; fly: FZ), CELSR (CELSR; FMI) and Van Gogh-like 39 (VANGL; STBM/VANG) and the cytosolic-submembrane proteins Dishevelled (DVL; DSH), 40Inversin/Diversin (INV/ANKRD6; DGO), and Prickle (PK). The PCP signaling system employs intra-and inter-41 cellular feedback interactions between its core components to establish their characteristic asymmetric 42 cellular distributions. In the most comprehensively-characterized system, the Drosophila wing blade, the 43 FZ-DSH-DGO complex localizes to intercellular junctions facing the VANG-PK complex in the adjacent 44 cell. FMI, an atypical cadherin, localizes to both sides of the apical junction and bridges the FZ and VANG 45 complexes between neighboring cells. These intercellular interactions are counterbalanced 46 intracellularly by reciprocal inhibitory interactions between the two complexes 1,2 . 47While traditionally depicted in flat epithelia that require directional motion or an oriented structure, it is less 48 clear how these proteins are organized or function in complex epithelia forming tubular structures 3 . The 49 developing pancreas consists of a network of tubes lined by polarized progenitors, the apical membranes of 50 which line the duct lumen 4,5 . This network is dynamic and remodeled throughout development while the 51 pancreatic epithelium grows and progenitors commit to various specialized fates 6 . At embryonic day 52 E10.5, multipotent progenitors are organized around discrete micro-lumens 7,8 . By E12.5, these micro-53 lumens have fused to form a mesh of interconnected ducts. Distal "Tip" progenitors committed to an 54 exocrine fate are restricted to the periphery while the core of this plexus contains bipotent "Trunk" 55 progenitors that subsequently either remain in the epithelium to form mature duct cells or delaminate 56 to give rise to endocrine cells. By birth, the duc...

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Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle

Cited by 18 publications

References 11 publications

High mitogenic stimulation arrests angiogenesis

High mitogenic stimulation arrests angiogenesis

Apical Restriction of the Planar Cell Polarity Component VANGL in Pancreatic Ducts Is Required to Maintain Epithelial Integrity

Apical restriction of the planar cell polarity component VANGL in pancreatic ducts is required to maintain epithelial integrity

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