Preparation and characterization of the collagen/cellulose nanocrystals/USPIO scaffolds loaded kartogenin for cartilage regeneration

Yang, Wei; Zheng, Yuanyuan; Chen, Jie; Zhu, Qiyu; Feng, Longbao; Lan, Yong; Zhu, Ping; Tang, Shuo; Guo, Rui

doi:10.1016/j.msec.2019.02.071

Cited by 43 publications

(22 citation statements)

References 52 publications

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“…CBFβ enters the nucleus and interacts with RUNX1 to form the CBFβ-RUNX1 complex that activates the transcription of chondrogenesis-related proteins and enhances cartilage ECM synthesis [22,23]. In the recent years, several groups have developed biomaterial platforms to promote the sustained release of KGN towards improved cartilage and osteochondral regeneration [24][25][26][27][28][29][30]. However, the use of coaxial electrospun aligned fibers promoting the sustained delivery of KGN to improve cartilage regeneration is currently underexplored.…”

Section: Introductionmentioning

confidence: 99%

Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering

Silva

Udangawa

Chen

et al. 2020

Materials Science and Engineering: C

101

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Electrospinning is a valuable technology for cartilage tissue engineering (CTE) due to its ability to produce fibrous scaffolds mimicking the nanoscale and alignment of collagen fibers present within the superficial zone of articular cartilage. Coaxial electrospinning allows the fabrication of coreshell fibers able to incorporate and release bioactive molecules (e.g., drugs or growth factors) in a controlled manner. Herein, we used coaxial electrospinning to produce coaxial poly(glycerol sebacate) (PGS)/poly(caprolactone) (PCL) aligned nanofibers (core:PGS/shell:PCL). The obtained scaffolds were characterized in terms of their structure, chemical composition, thermal properties, mechanical performance and in vitro degradation kinetics, in comparison to monoaxial PCL aligned fibers and respective non-aligned controls. All the electrospun scaffolds produced presented average fiber diameters within the nanometer-scale and the core-shell structure of the composite fibers was clearly confirmed by TEM. Additionally, fiber alignment significantly increased (>2-fold) the elastic modulus of both coaxial and monoxial scaffolds. Kartogenin (KGN), a small molecule known to promote mesenchymal stem/stromal cells (MSC)

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Section: Introductionmentioning

confidence: 99%

Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering

Silva

Udangawa

Chen

et al. 2020

Materials Science and Engineering: C

101

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“…Increasing evidence has indicated that KGN is able to protect articular cartilage from OA-induced degeneration 20 , 21 . For instance, Fan et al demonstrated that intra-articular injection of KGN conjugated polyurethane nanoparticles relieved the progression of post-traumatic OA in mice 22 .…”

Section: Discussionmentioning

confidence: 99%

Kartogenin prevents cartilage degradation and alleviates osteoarthritis progression in mice via the miR-146a/NRF2 axis

et al. 2021

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Osteoarthritis (OA) is a common articular degenerative disease characterized by loss of cartilage matrix and subchondral bone sclerosis. Kartogenin (KGN) has been reported to improve chondrogenic differentiation of mesenchymal stem cells. However, the therapeutic effect of KGN on OA-induced cartilage degeneration was still unclear. This study aimed to explore the protective effects and underlying mechanisms of KGN on articular cartilage degradation using mice with post-traumatic OA. To mimic the in vivo arthritic environment, in vitro cultured chondrocytes were exposed to interleukin-1β (IL-1β). We found that KGN barely affected the cell proliferation of chondrocytes; however, KGN significantly enhanced the synthesis of cartilage matrix components such as type II collagen and aggrecan in a dose-dependent manner. Meanwhile, KGN markedly suppressed the expression of matrix degradation enzymes such as MMP13 and ADAMTS5. In vivo experiments showed that intra-articular administration of KGN ameliorated cartilage degeneration and inhibited subchondral bone sclerosis in an experimental OA mouse model. Molecular biology experiments revealed that KGN modulated intracellular reactive oxygen species in IL-1β-stimulated chondrocytes by up-regulating nuclear factor erythroid 2-related factor 2 (NRF2), while barely affecting its mRNA expression. Microarray analysis further revealed that IL-1β significantly up-regulated miR-146a that played a critical role in regulating the protein levels of NRF2. KGN treatment showed a strong inhibitory effect on the expression of miR-146a in IL-1β-stimulated chondrocytes. Over-expression of miR-146a abolished the anti-arthritic effects of KGN not only by down-regulating the protein levels of NRF2 but also by up-regulating the expression of matrix degradation enzymes. Our findings demonstrate, for the first time, that KGN exerts anti-arthritic effects via activation of the miR-146a-NRF2 axis and KGN is a promising heterocyclic molecule to prevent OA-induced cartilage degeneration.

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“…Recently, Huang et al published a study involving the preparation of magnetic nanocomposite hydrogels from Fe 3 O 4 , polyvinyl alcohol (PVA) and type II collagen, which exhibited good physical properties as well as good swelling behavior and cell compatibility [ 298 ]. In another work, Yang et al fabricated scaffolds of cross-linked collagen/cellulose nanocrystals in which ultra-small IONPs functionalized with the chondroinductive small molecule kartogenin were incorporated [ 299 ]. The resulting microenvironment stimulated the growth and differentiation of BM-MSCs and thus the formation of chondrocytes.…”

Section: Hard and Connective Tissue Regeneration And Engineeringmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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Preparation and characterization of the collagen/cellulose nanocrystals/USPIO scaffolds loaded kartogenin for cartilage regeneration

Cited by 43 publications

References 52 publications

Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering

Kartogenin-loaded coaxial PGS/PCL aligned nanofibers for cartilage tissue engineering

Kartogenin prevents cartilage degradation and alleviates osteoarthritis progression in mice via the miR-146a/NRF2 axis

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

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