Preparation and characterization of site-specific dechlorinating microbial inocula capable of complete dechlorination enriched in anaerobic microcosms amended with clay mineral

Nagymáté, Zsuzsanna; Jurecska, Laura; Romsics, Csaba; Tóth, Ferenc; Bódai, Viktória; Mészáros, Éva; Erdélyi, Balázs; Màrialigeti, Károly

doi:10.1007/s11274-020-2806-7

Cited by 6 publications

(2 citation statements)

References 63 publications

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“…The same progress was observed for Dehalococcoides, although, in this case, a weaker increase was reported. Similar trends of microbial communities were found either natural [27,71] or modified by the addition of carbon substrates [54,64]. In such complex microbial communities, many competitions can be found that are partially discussed above.…”

Section: Molecular Biological Analysessupporting

confidence: 66%

Thermally Enhanced Biodegradation of TCE in Groundwater

Najmanová

Steinová

Czinnerová

et al. 2022

Water

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In situ remediation is usually restricted by temperature, lack of substrate for reductive dechlorination (anaerobic respiration), the presence of dehalogenating microorganisms, and specific bedrock conditions. In this work, trichloroethene (TCE) degradation was studied by a number of methods, from physical–chemical analyses to molecular biological tools. The abundance changes in dechlorinating bacteria were monitored using real-time PCR. The functional genes vcrA and bvcA as well as the 16S rRNA specific for representatives of genera Dehalococcoides, Dehalobacter, and Desulfitobacterium were monitored. Furthermore, the sulfate-reducing bacteria and denitrifying bacteria were observed by amplifying the functional genes apsA and nirK. The elevated temperature and the substrate (whey) addition significantly affected TCE dechlorination. The chlorine index decreased after nine weeks from 2.5 to 0.1 at 22 °C, to 1.1 at 17 °C and 1.7 at 12 °C and complete dechlorination was achieved at 22 °C with whey addition. The achieved results of this work show the feasibility and effectiveness of biological dechlorination of TCE enhanced with elevated temperature and whey addition.

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Section: Molecular Biological Analysessupporting

confidence: 66%

Thermally Enhanced Biodegradation of TCE in Groundwater

Najmanová

Steinová

Czinnerová

et al. 2022

Water

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“…Other than the well-known and highly abundant organohalide-respiring D. mccartyi, additional genera identified in the SG sample A0 have been implicated in dechlorinating processes. Amongst them, the Euryarchaeota, genus Methanosarcina is a PCE and TCE dehalogenator [22,23] while the genus Methanosphaerula has been recently described as a member of an anaerobic dechlorinating enrichment [75]. Species belonging to genus Geomonas (Geobacter) are often present in environments tainted with chlorinated ethenes and play a pivotal role in anaerobically dechlorination of PCE to cis-DCE [12,21,55], as does Sulfurospirillum [20].…”

Section: Microbial Compositionmentioning

confidence: 99%

Bioremediation of a Polluted Groundwater: Microbial Community Comparison of Treated and Untreated Aquifer through Next Generation Sequencing

Pretto

Sanseverino

Demichelis

et al. 2022

Water

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Bioremediation is an active process for the detoxification of polluted ambient media employing the metabolism of microbes, while natural attenuation relies on physical, chemical and biological processes occurring without human intervention. A shallow aquifer (A0) was treated using a bioremediation approach through the amendment of whey to detoxify the most abundant contaminants: 1,1,2,2- tetrachloroethane (1,1,2,2-TeCA), perchloroethene (PCE) and trichloroethene (TCE). A deeper aquifer (A1), showing lower concentration of the contaminants, was left untreated. In A0, a concomitant decrease of more chlorinated molecules 1,1,2,2-TeCA, PCE and TCE and an increase of less halogenated molecules such as trichloroethane (1,1,2-TCA), cis-dichloroethene (cis-DCE) and vinyl chloride (VC) were observed, suggesting that a reductive dechlorination took place. In contrast, the aquifer A1 did not show a significant decrease of contaminants during this period. A metagenomic approach (shot gun and 16S rRNA gene) was then used to investigate the microbial population of the two aquifers. A massive presence of the dehalogenator Dehalococcoides mccartyi (D. mccartyi) and a spectrum of different Geobacter species were detected in A0, after the treatment. The metagenome assembly of shotgun (SG) data further indicated a significant presence of methanogenic archaea, most likely from class Methanomassiliicoccales, at a level comparable to that of D. mccartyi. Instead, A1 was characterized by the species Burkholderia, Curvibacter and Flavobacterium. These results indicate that the autochthonous microbial consortia reflected the geochemistry of the two aquifers, with a dominant population thriving in an anoxic and nutrient rich environment implicated in reductive dehalogenation in A0 and a more diverse population, not able to decompose the pollutants, in A1.

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Field test of a bioaugmentation agent for the bioremediation of chlorinated ethene contaminated sites

Krett,

Romsics,

Jurecska

et al. 2024

BIOLOGIA FUTURA

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Chlorinated ethenes are toxic compounds that were widely used in the past, and their improper handling and storage caused notable pollutions worldwide. In situ bioremediation by reductive dechlorination of bacteria is a cost-effective and ecologically friendly way to eliminate these pollutions. During the present study, the efficiency of a previously developed bioaugmentation agent combined with biostimulation was tested under field conditions in contaminated soil. Furthermore, the preservation of dechlorinating ability was also investigated in a long-term experiment. Initially, aerobic conditions were present in the groundwater with possible presence of anaerobic micro-niches providing habitat for Brocadia related anammox bacteria. “Candidatus Omnitrophus” was also identified as a dominant member of community then. Significant changes were detected after the biostimulation, anaerobic conditions established and most of the dominant OTUs were related to fermentative taxa (e.g. Clostridium, Trichococcusand Macillibacteroides). Dominant presence of vinyl-chloride coupled with the lack of vinyl-chloride reductase gene was observed. The most notable change after the bioaugmentation was the significant decrease in the pollutant quantities and the parallel increase in the vcrA gene copy numbers. Similar to post-biostimulation state, fermentative bacteria dominated the community. Bacterial community composition transformed considerably with time after the treatment, dominance of fermentative—mainly Firmicutes related—taxa decreased and chemolithotrophic bacteria became abundant, but the dechlorinating potential of the community remained and could be induced by the reappearance of the pollutants even after 4 years.

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Preparation and characterization of site-specific dechlorinating microbial inocula capable of complete dechlorination enriched in anaerobic microcosms amended with clay mineral

Cited by 6 publications

References 63 publications

Thermally Enhanced Biodegradation of TCE in Groundwater

Thermally Enhanced Biodegradation of TCE in Groundwater

Bioremediation of a Polluted Groundwater: Microbial Community Comparison of Treated and Untreated Aquifer through Next Generation Sequencing

Field test of a bioaugmentation agent for the bioremediation of chlorinated ethene contaminated sites

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