Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate

Pieper, Jeroen; Oosterhof, Arie; Dijkstra, P.J.; Veerkamp, J.H.; Kuppevelt, A.H.M.S.M. van

doi:10.1016/s0142-9612(98)00240-3

Cited by 384 publications

(258 citation statements)

References 35 publications

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“…Non treated collagen has 32 free amine groups per 1000 amino acid residues (100% free amine groups). 24 Pieper et al reported that similar collagen-GAG scaffolds retained 68% of free amine groups when crosslinked by DHT and EDC/NHS compared to EDC/NHS treatment, which retained 63%. 24 DHT partially denatures collagen by breaking the hydrogen bonding needed to maintain the collagen triple-helix structure.…”

Section: Introductionmentioning

confidence: 99%

“…24 DHT partially denatures collagen by breaking the hydrogen bonding needed to maintain the collagen triple-helix structure. 24,25 However, although DHT appears to have little effect on scaffold structure, it is not known if it affects the binding and release of insulin-like growth factor-1 (IGF-1) from this type of scaffold, and therefore we compared scaffolds cross-linked via DHT and EDC/NHS (þDHT) with scaffolds cross-linked by EDC/NHS only (ÀDHT).…”

Section: Introductionmentioning

confidence: 99%

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Binding and Release Characteristics of Insulin-Like Growth Factor-1 from a Collagen–Glycosaminoglycan Scaffold

Mullen

Best

Brooks

et al. 2010

Tissue Engineering Part C: Methods

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Tissue engineering is a promising technique for cartilage repair, but to optimize novel scaffolds before clinical trials, it is necessary to determine their characteristics for binding and release of growth factors. Toward this goal, a novel, porous collagen-glycosaminoglycan scaffold was loaded with a range of concentrations of insulinlike growth factor-1 (IGF-1) to evaluate its potential as a controlled delivery device. The kinetics of IGF-1 adsorption and release from the scaffold was demonstrated using radiolabeled IGF-1. Adsorption was rapid, and was approximately proportional to the loading concentration. Ionic bonding contributed to this interaction. IGF-1 release was studied over 14 days to compare the release profiles from different loading groups. Two distinct phases occurred: first, a burst release of up to 44% was noted within the first 24 h; then, a slow, sustained release (13%-16%) was observed from day 1 to 14. When the burst release was subtracted, the relative percentage of remaining IGF-1 released was similar for all loading groups and broadly followed t ½ kinetics until approximately day 6. Scaffold cross-linking using dehydrothermal treatment did not affect IGF-1 adsorption or release. Bioactivity of released IGF-1 was confirmed by seeding scaffolds (preadsorbed with unlabeled IGF-1) with human osteoarthritic chondrocytes and demonstrating increased proteoglycan production in vitro.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Binding and Release Characteristics of Insulin-Like Growth Factor-1 from a Collagen–Glycosaminoglycan Scaffold

Mullen

Best

Brooks

et al. 2010

Tissue Engineering Part C: Methods

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“…Bioscaffolds were prepared using type I collagen fibrils derived from bovine Achilles' tendon, 26 as previously described. 27,28 A 0.8% (w/v) suspension of insoluble type I collagen in 0.25 M acetic acid was prepared.…”

Section: Methodsmentioning

confidence: 99%

Heparinized collagen scaffolds with and without growth factors for the repair of diaphragmatic hernia

et al. 2013

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“…Insoluble type I collagen was derived from pig Achilles' tendon using neutral salt and dilute acid extractions with enzyme treatment [13,23]. Type III collagen was removed by centrifugation after salt fractionation, and type I collagen was drawn off the lower portion.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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a b s t r a c tRotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, while commercially-available synthetic scaffolds are often inadequate at inducing tenogenic differentiation. The aim of this study is to develop an advanced 3D aligned collagen/silk scaffold (ACS) and investigate its efficacy in a rabbit massive rotator cuff tear model. ACS has similar 3D alignment of collagen fibers as natural tendon with superior mechanical characteristics. Based on ectopic transplantation studies, the optimal collagen concentration (10 mg/ml), pore diameter (108.43 ± 7.25 lm) and porosity (97.94 ± 0.08%) required for sustaining a stable macro-structure conducive for cellular infiltration was determined. Within in vitro culture, tendon stem/progenitor cells (TSPCs) displayed spindle-shaped morphology, and were well-aligned on ACS as early as 24 h. TSPCs formed intercellular contacts and deposited extracellular matrix after 7 days. With the in vivo rotator cuff repair model, the regenerative tendon of the ACS group displayed more conspicuous native microstructures with larger diameter collagen fibrils (48.72 ± 3.75 vs. 44.26 ± 5.03 nm) that had better alignment and mechanical properties (139.85 ± 49.36 vs. 99.09 ± 33.98 N) at 12 weeks post-implantation. In conclusion, these findings demonstrate the positive efficacy of the macroporous 3D aligned scaffold in facilitating rotator cuff tendon regeneration, and its practical applications for rotator cuff tendon tissue engineering. Statement of SignificanceMassive rotator cuff tear is one of the most common shoulder injuries, and poses a formidable clinical challenge to the orthopedic surgeon. Tissue engineering of tendon can potentially overcome the problem. However, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for repairing massive rotator cuff tendon injuries need to be developed. In this study, we developed a novel macroporous 3D aligned collagen/silk scaffold, and demonstrated that this novel scaffold enhanced the efficacy of rotator cuff tendon regeneration by inducing aligned supracellular structures similar to natural tendon, which in turn enhanced http://dx

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Preparation and characterization of porous crosslinked collagenous matrices containing bioavailable chondroitin sulphate

Cited by 384 publications

References 35 publications

Binding and Release Characteristics of Insulin-Like Growth Factor-1 from a Collagen–Glycosaminoglycan Scaffold

Binding and Release Characteristics of Insulin-Like Growth Factor-1 from a Collagen–Glycosaminoglycan Scaffold

Heparinized collagen scaffolds with and without growth factors for the repair of diaphragmatic hernia

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

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