Preparation and characterization of poly(amidoamine) dendrimers functionalized with a rhenium carbonyl complex and PEG as new IR probes for carbonyl metallo immunoassay

Heldt, Jan‐Martin; Fischer‐Durand, Nathalie; Salmain, Michèle; Vessières, Anne; Jaouen, Gérard

doi:10.1016/j.jorganchem.2004.09.035

Cited by 60 publications

(45 citation statements)

References 32 publications

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“…Metalcyclopentadienyl-methylalcohols 9 a, 9 b and 9 c were prepared by reduction of the corresponding formyl compounds CpFeA C H T U N G T R E N N U N G (C 5 H 4 ÀCHO), [57] CpRuA C H T U N G T R E N N U N G (C 5 H 4 ÀCHO) [57] and Re(CO) 3 A C H T U N G T R E N N U N G (C 5 H 4 ÀCHO). [58] Papain from papaya latex (EC 3.4.22.2) was obtained as a suspension in acetate buffer (P3125, Sigma) and further purified by affinity chromatography by following the method of Blumberg et al, [59] with slight modification as Gly-GlyTyr-Arg (Sigma) was used as immobilized inhibitor on agarose instead of Gly-Gly-TyrA C H T U N G T R E N N U N G (Bzl)-Arg as initially described. Under these conditions, 3 mg of fully active papain (the number of SH to protein was equal to 1, as fluorimetrically assayed by using ThioGlo 1 reagent [60] ) was typically recovered from 10 mg of starting material.…”

Section: Methodsmentioning

confidence: 99%

Cysteine‐Specific, Covalent Anchoring of Transition Organometallic Complexes to the Protein Papain from Carica papaya

et al. 2007

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Site-directed and covalent introduction of various transition metal-organic entities to the active site of the cysteine endoproteinase, papain, was achieved by treatment of this enzyme with a series of organometallic maleimide derivatives specially designed for the purpose. Kinetic studies made it clear that time-dependent irreversible inactivation of papain occurred in the presence of these organometallic maleimides as a result of Michael addition of the sulfhydryl of Cys25. The rate and mechanism of inactivation were highly dependent on the structure of the organometallic entity attached to the maleimide group. Combined ESI-MS and IR analysis indicated that all the resulting papain adducts contained one organometallic moiety per protein molecule. This confirmed that chemospecific introduction of the metal complexes was indeed achieved. Thus, three novel reagents for heavy-atom derivatization of protein crystals, which include ruthenium, rhenium and tungsten, are now available for the introduction of electron-dense scatterers for phasing of X-ray crystallographic data.

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Section: Methodsmentioning

confidence: 99%

Cysteine‐Specific, Covalent Anchoring of Transition Organometallic Complexes to the Protein Papain from Carica papaya

et al. 2007

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Dendrimers represent a key stage in the ongoing evolution of macromolecular chemistry, which is a result of the wide range of applications that have been foreseen for such materials in biomedical science. Applications have included solubility enhancement, [16][17][18][19] MRI contrast agents, [20][21][22][23][24][25][26][27] neutron capture therapy, [28][29][30][31][32][33][34] gene therapy, [35][36][37][38][39][40][41] drug delivery, [42][43][44] nanocomposites, [45][46][47][48] and photodynamic therapy. [49]…”

Section: Introductionmentioning

confidence: 99%

Dendritic Polyglycerols for Biomedical Applications

et al. 2009

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The application of nanotechnology in medicine and pharmaceuticals is a rapidly advancing field that is quickly gaining acceptance and recognition as an independent area of research called "nanomedicine". Urgent needs in this field, however, are biocompatible and bioactive materials for antifouling surfaces and nanoparticles for drug delivery. Therefore, extensive attention has been given to the design and development of new macromolecular structures. Among the various polymeric architectures, dendritic ("treelike") polymers have experienced an exponential development due to their highly branched, multifunctional, and well-defined structures. This Review describes the diverse syntheses and biomedical applications of dendritic polyglycerols (PGs). These polymers exhibit good chemical stability and inertness under biological conditions and are highly biocompatible. Oligoglycerols and their fatty acid esters are FDA-approved and are already being used in a variety of consumer applications, e.g., cosmetics and toiletries, food industries, cleaning and softening agents, pharmaceuticals, polymers and polymer additives, printing photographing materials, and electronics. Herein, we present the current status of dendritic PGs as functional dendritic architectures with particular focus on their application in nanomedicine, in drug, dye, and gene delivery, as well as in regenerative medicine in the form of non-fouling surfaces and matrix materials.

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“…In these, the synthesis of PEGylated dendrimers and importance of PEGylation were discussed. [11][12][13][14] …”

Section: Original Articlementioning

confidence: 99%

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Khambete

2017

AJP

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Aim: To check the effect of polyethylene glycol (PEG) chain length on PEGylation of dendrimers. Materials and Methods: In the present work, we have synthesized different PEGylated polyamidoamine dendrimers using six different PEG chains, i.e., PEG -200, 300, 400, 600, 2000, and 6000. The PEGylated dendrimers were evaluated for color reaction ultraviolet, infrared, and nuclear magnetic resonance studies and compared with standard data. Results and Discussion: The plain dendrimers give violet color due to free NH2 groups. The intensity of violet color of 4.0 G dendrimers decreases on PEGylation, due to attachment of PEG chain on free NH2 groups which is responsible for violet color. The change in λmax values from 283 to 353 nm was observed, which shows the change in structure of dendrimers. On comparing, it was found that PEG 400 and 600 shows value near their expected values, i.e., 19,150 and 19,780, respectively. Conclusion: From the results of the present study, it can be concluded that PEGylation using PEG 400 and 600 gives a considerable level of attachment of PEG to dendrimers as compared to other PEG chains.

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Preparation and characterization of poly(amidoamine) dendrimers functionalized with a rhenium carbonyl complex and PEG as new IR probes for carbonyl metallo immunoassay

Cited by 60 publications

References 32 publications

Cysteine‐Specific, Covalent Anchoring of Transition Organometallic Complexes to the Protein Papain from Carica papaya

Cysteine‐Specific, Covalent Anchoring of Transition Organometallic Complexes to the Protein Papain from Carica papaya

Dendritic Polyglycerols for Biomedical Applications

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