Preparation and characterization of pH-sensitive proteinoid microspheres for the oral delivery of methotrexate

Anurag, Kumar; Rao, K. Panduranga

doi:10.1016/s0142-9612(97)00188-9

Cited by 67 publications

(29 citation statements)

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“…Recently, proteinoid particles were studied by several groups as new drug delivery systems [ 14 ]–[ 16 ]. The thermal condensation of amino acids into proteinoids was first described and characterized by Fox and Harada [ 17 ]–[ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…When proteinoids are incubated in an aqueous solution they form hollow particles that range in size according to the environment conditions [ 21 ]. Since proteinoid particles are considered biodegradable, non-immunogenic and non-toxic, they can be used as a delivery vehicle in the body [ 16 ]. In this work, a new version of proteinoid is synthesized and studied.…”

Section: Introductionmentioning

confidence: 99%

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Engineering of near infrared fluorescent proteinoid-poly(L-lactic acid) particles for in vivo colon cancer detection

et al. 2014

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BackgroundThe use of near-infrared (NIR) fluorescence imaging techniques has gained great interest for early detection of cancer owing to the negligible absorption and autofluorescence of water and other intrinsic biomolecules in this region. The main aim of the present study is to synthesize and characterize novel NIR fluorescent nanoparticles based on proteinoid and PLLA for early detection of colon tumors.MethodsThe present study describes the synthesis of new proteinoid-PLLA copolymer and the preparation of NIR fluorescent nanoparticles for use in diagnostic detection of colon cancer. These fluorescent nanoparticles were prepared by a self-assembly process in the presence of the NIR dye indocyanine green (ICG), a FDA-approved NIR fluorescent dye. Anti-carcinoembryonic antigen antibody (anti-CEA), a specific tumor targeting ligand, was covalently conjugated to the P(EF-PLLA) nanoparticles through the surface carboxylate groups using the carbodiimide activation method.Results and discussionThe P(EF-PLLA) nanoparticles are stable in different conditions, no leakage of the encapsulated dye into PBS containing 4% HSA was detected. The encapsulation of the NIR fluorescent dye within the P(EF-PLLA) nanoparticles improves significantly the photostability of the dye. The fluorescent nanoparticles are non-toxic, and the biodistribution study in a mouse model showed they evacuate from the body over 24 h. Specific colon tumor detection in a chicken embryo model and a mouse model was demonstrated for anti-CEA-conjugated NIR fluorescent P(EF-PLLA) nanoparticles.ConclusionsThe results of this study suggest a significant advantage of NIR fluorescence imaging using NIR fluorescent P(EF-PLLA) nanoparticles over colonoscopy. In future work we plan to broaden this study by encapsulating cancer drugs such as paclitaxel and/or doxorubicin, within these biodegradable NIR fluorescent P(EF-PLLA) nanoparticles, for both detection and therapy of colon cancer.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineering of near infrared fluorescent proteinoid-poly(L-lactic acid) particles for in vivo colon cancer detection

et al. 2014

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“…In addition, proteinoids are relatively easy to synthesize and characterize35, 46–48 and the theoretical foundation for their self‐assembly processes have been formulated 49. Historically, PM research has focused on cell‐like compartmentalization and protocell‐like function as the role of PMs in the evolution from abiotic to biotic primordial Earth conditions, more recently they have been used as small molecule drug release agents 41, 50. PMs can also be formed with other materials incorporated into their base structure 42, 51.…”

Section: Discussionmentioning

confidence: 99%

New polymer syntheses part: 54 novel conducting polymers and copolymers based on 4‐Teriary butyl‐cyclohexanone moiety in the main chain

Aly

2011

J of Applied Polymer Sci

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A new interesting class of conducting polymer and copolymers based on 4-teriary butyl-cyclohexanone in the main chain has been synthesized by solution polycodensation of terephthalaldehyde with 4-teriary butylcyclohexanone and/or cycloalkanone derivatives. The model compound I was synthesized from the 4-teriary butyl-cyclohexanone with benzaldehyde, and its structure was confirmed by elemental and spectral analyses. The resulting polymer and copolymers were characterized by elemental and spectral analyses including Fourier transform infrared spectrometer (FT-IR) and nuclear magnetic resonance ( 1 H-NMR), beside solubility and viscometry measurements. The thermal properties of those polymer and copolymers were evaluated by thermogravimetric analysis (TGA) and differential scanning calorimeter (DSC) measurements and correlated to their structural units. X-ray analysis showed that it has some degree of crystallinity in the region 2y ¼ 5-60. The UV-visible spectra of some selected polymers were measured in dimethyl sulfoxide (DMSO) solution and showed absorption bands in the range 253-398 nm, due to n-p* and p-p* transition. The morphological properties of selected examples were tested by scanning electron microscope (SEM). Moreover, the electrical conductivities and the doping with iodine were tested.

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“…In addition, they are relatively easy to synthesize and characterize 27–30. After 50 years of study, the polymers are well understood: including the theoretical foundation for the assembly process,12 membrane‐like electrical potential,31–34 compartmentalization and the responses of internal compartments to changes in temperature and pH,5 and using PMs to deliver therapeutic agents (e.g., Refs 35–3738…”

Section: Introductionmentioning

confidence: 99%

“…The difficulty however is finding suitable release triggers. One group37 has described a pH sensitive PM that can be used for intestinal delivery via oral administration. Another study38 described the use of reducible PMs to trigger delivery.…”

Section: Introductionmentioning

confidence: 99%

Triggered release from peptide‐proteinoid microspheres

Quirk

2009

J Biomedical Materials Res

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Proteinoid microspheres (PM) are unusual polymers formed by the thermal condensation of amino acids. Although they have been studied for over 60 years, they are only now beginning to garner interest as controlled release agents. Although they are very biocompatible, it has been problematic to design useful triggers that release small molecules from PM interiors. This has severely limited their usefulness. In the present study, short peptides have been successfully incorporated into PMs during their formation. The resulting hybrid peptide-PMs can release their interior content when hydrolyzed by a proteinase. Specifically, if a matrix metalloproteinase (MMP) cleavage site peptide is incorporated into a PM, the peptide-PM will release interior contents only in the presence of the MMP recognizing the cleavage peptide. The release rate can be determined by the concentration of the peptide in the PM synthesis mixture. This potentially makes peptide-PMs useful for delivering inhibitors or drugs into acute and chronic wounds, periodontal disease sites, and other disease states involving the fine-tuned regulation of proteinases.

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Preparation and characterization of pH-sensitive proteinoid microspheres for the oral delivery of methotrexate

Cited by 67 publications

References 0 publications

Engineering of near infrared fluorescent proteinoid-poly(L-lactic acid) particles for in vivo colon cancer detection

Engineering of near infrared fluorescent proteinoid-poly(L-lactic acid) particles for in vivo colon cancer detection

New polymer syntheses part: 54 novel conducting polymers and copolymers based on 4‐Teriary butyl‐cyclohexanone moiety in the main chain

Triggered release from peptide‐proteinoid microspheres

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