Preparation and Characterization of Olaquindox Polyclonal Antibody

Abstract

2-[N-(2-Hydroxyethyl)-carbamoyl]-3-methyl-oxoquinoxaline-1,4-oxides (OLA) was first mono-esterificated by succinic acid anhydride, and was then turned into a derivative with carboxyl. The synthetic product was purified by recrystallization with a yield of 52.47%. Its chemical structure was determined by NMR, IR and MS spectra. The determined structure was used to synthese OLA hapten, which had the molecular structural characteristics of OLA. The OLA hapten and bovine serum albumin (BSA) were conjugated using the activated ester method, while the hapten and ovalbumin (OVA) were coupled by the mixed anhydride method. The UV scanning and infrared spectrum results showed that the hapten and the carrier protein were successfully coupled to BSA and OVA, with the combined ratios of 3.8∶1 and 5∶1, respectively. OLA-BSA was used as the immunogen to immunize four New Zealand white rabbits, and the high titer anti-serum produced relatively. The titers were 1∶6400, 1∶1600, 1∶12800 and 1∶6400, respectively, determined by indirect ELISA OLA-OVA as the coating antigen. The intermediate inhibition concentration (IC 50 ) of OLA in the indirect ELISA test was 743.3 ng/mL. The lowest detection limit (IC 20 ) was 5.71 ng/mL. High-purity OLA polyclonal antibody has been obtained by saturated ammonium sulfate and ion-exchange chromatography.

A novel molecularly imprinted electrochemiluminescence sensor for isoniazid detection

“…[29][30][31][32][33] Poisoning incidents, even death, have occurred occasionally due to overdose of isoniazid (isonicotinic acid hydrazide, INH), which is a wonder drug for tuberculosis and is one of the top pharmaceuticals that has changed the world. [34][35][36][37][38] Therefore, controlling the dose of INH is very important to sufferers in clinical chemistry, which has prompted study of analytical methods for INH, including titrimetric, 39,40 colorimetric, [41][42][43] spectrophotometric, [44][45][46][47][48][49][50] atomic absorption spectrometric, 51 fluorimetric, [52][53][54] high performance liquid chromatographic, [55][56][57][58][59][60][61][62] capillary electrophoresis, 63,64 chromatographic, 65,66 mass spectrometric, 67 chemiluminescence, [68][69][70][71][72][73][74][75][76][77]…”

Determination of quinoxaline antibiotics in fish feed by enzyme-linked immunosorbent assay using a monoclonal antibody

“…22 Immunoassays have been developed for OLA residue detection. 3,[23][24][25][26] However, few researchers have used monoclonal antibody based-ELISA for QCT and MEQ detection. The simultaneous detection of multi-residue quinoxaline antibiotics has been reported.…”

hydrolysis of Scutellarin and Related Glycosides to Scutellarein and the Corresponding Aglycones

“…2,3 SCUE has better solubilities and much more superior absolute bioavailability than SCU in vivo. 4 Recent reports disclosed that SCUE was the main metabolite of SCU when it was administered to rats. [5][6][7][8] Moreover, it was reported that SCUE had other important physio-and pharmacological activities such as preventing vascular endothelial dysfunction, 9 inhibiting the proliferation of human retinal endothelial cells (HREC), 10 inhibiting SARS-CoV (Severe acute respiratory syndrome-coronavirus), 11 decreasing neurological deficit score and cerebral infarction volume, 12 and selective cytotoxicities towards cancer cells.…”