Preparation and Characterization of Nickel Oxide for Antibacterial Properties

Abstract

NiO thin films of different preferred orientations have been growth successfully by sol-gel technique using methanol solvent. The resulting film was analyzed by X-ray diffraction. Phase analysis of the sample was carried out by XRD shown the relative severity of the peak (111) represents a high crystalline of nickel oxide structure. Antibacterial properties of NiO based film against two types of gram-negative bacteria Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) have been evaluated using mac varland method. The antimicrobial activity of the sample was determined through counting the number of colonies on each plate which was reported as colony-forming unit (CFU) per milliliter. Results showed that NiO has high activation efficiency against (E. coli) and (S. aureus).