Preparation and characterization of nano‐hydroxyapatite/chitosan composite scaffolds

Abstract

A novel nano-hydroxyapatite (HA)/chitosan composite scaffold with high porosity was developed. The nano-HA particles were made in situ through a chemical method and dispersed well on the porous scaffold. They bound to the chitosan scaffolds very well. This method prevents the migration of nano-HA particles into surrounding tissues to a certain extent. The morphologies, components, and biocompatibility of the composite scaffolds were investigated. Scanning electron microscopy, porosity measurement, thermogravimetric analysis, X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier transformed infrared spectroscopy were used to analyze the physical and chemical properties of the composite scaffolds. The biocompatibility was assessed by examining the proliferation and morphology of MC 3T3-E1 cells seeded on the scaffolds. The composite scaffolds showed better biocompatibility than pure chitosan scaffolds. The results suggest that the newly developed nano-HA/chitosan composite scaffolds may serve as a good three-dimensional substrate for cell attachment and migration in bone tissue engineering.

Calcium orthophosphate-based biocomposites and hybrid biomaterials

“…Thus, the biocompatibility of chitosan in osteoblast cell culture was significantly improved by addition of nano-HA [582]. Similar finding is valid for nano-HA/polyamide biocomposites [531].…”

Biocomposites and hybrid biomaterials based on calcium orthophosphates

“…694 For example, the biocompatibility of chitosan in osteoblast cell culture was significantly improved by the addition of nano-sized HA. 695 Similar findings are valid for nanodimensional HA/polyamide biocomposites. 630 Further details on nanodimensional biocomposites might be found in an excellent review in reference 36.…”

“…Various biocomposites of calcium orthophosphates with chitosan 298,482,500,512,527,565,[645][646][647][648][649][650][651][652][653]674,675,690,695,799,807,[811][812][813][814][815][816][817][818][819][820][821][822][823][824][825][826][827][828] and chitin 232,476,612,[829][830][831][832][833] are also very popular. For example, a solutionbased method was developed to combine HA powders with chitin, in which the ceramic particles were uniformly dispersed.…”

The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects

“…Others have reported that fibrous scaffolds had a much greater surface-to-volume ratio than scaffolds with solid pore walls, which might have further increased protein adsorption capacity 21,22) .…”