Preparation and characterization of lauryl methacrylate-based monolithic microbore column for reversed-phase liquid chromatography

Shu, Shin; Kobayashi, Hiroharu; Kojima, Norihisa; Sabarudin, Akhmad; Umemura, Tomonari

doi:10.1016/j.chroma.2011.05.104

Cited by 49 publications

(40 citation statements)

References 43 publications

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“…LMA-EDMA organic monolithic column was already applied for the peptides separation [26,27], and this organic monolith exhibited abundant mesopores and macropores, which may be suitable for the intact protein separation [26,28]. A total of 18 monolithic capillary columns with 100 m i.d.…”

Section: Lc Separation Performance Of Poss-based Hybrid Monolithic Camentioning

confidence: 99%

Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns

Ou¹,

Liu²,

Lin³

et al. 2013

Journal of Chromatography A

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Section: Lc Separation Performance Of Poss-based Hybrid Monolithic Camentioning

confidence: 99%

Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns

Ou¹,

Liu²,

Lin³

et al. 2013

Journal of Chromatography A

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“…Prior to the polymerization, the inner wall of a steel tubing was pretreated with MAPS to afford olefins on the tube inner wall for covalent binding of the polymer monolith, with procedure as described by Shu et al [30]. First of all, steel tubing was washed with distilled water, filled with 0.2 M NaOH for 2 x 30 minutes, rinsed with water 3 times, then filled with 0.2 M HCl for 2 x 30 minutes, and rinsed with water followed by acetone.…”

Section: Procedures Preparation Of Monolith Column Poly-(mate-co-vbc-cmentioning

confidence: 99%

“…Microbore column can be easily connected to commercially available standard HPLC system with minor modification. Although requiring more attention to the uneven heating along the diameter of the column during the polymerization process, microbore columns combine the advantages of both capillary and conventional-size columns, that is low sample and reagent consumption, reduced waste, shorten analysis time, and enhanced sensitivity from the small amount of sample [13,28,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

Malis

Tasfiyati

Iftitah

et al. 2015

J. Pure App. Chem. Res.

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A polymer monolithic column, poly-(methacrylatoethyltrimethylammonium chloride-covinylbenzyl chloride-co-ethylene dimethacrylate) or poly-(MATE-co-VBC-co-EDMA) was successfully prepared in the current study by one-step thermally initiated in situ polymerization, confined in a steel tubing of 0.5 mm i.d. and 1/16" o.d. The monoliths were prepared from methacrylatoethyltrimethylammonium chloride (MATE) and vinylbenzyl chloride (VBC) as monomer and ethylene dimethacrylate (EDMA) as crosslinker using a binary porogen system of 1-propanol and 1,4-butanediol. The inner wall of steel tubing was pretreated with 3-methacryloxypropyl-trimethoxysilane (MAPS). In order to obtain monolith with adequate column efficiency and low flow resistance, some parameters such as total monomer concentration (%T) and crosslinker concentration (%C) were optimized. The morphology of this monolith was assessed by scanning electron microscopy (SEM). The properties of the monolithic column, such as permeability, binding capacity, and pore size distribution were also characterized in detail. From the results of the characterization of all monolith variation, monolith with %T 30 %C 50 and %T 35 %C 50 give the best characteristic. These monoliths have high permeability, adequate molecular recognition sites (represented with binding capacity value of over 20 mg/mL), and have over 80% flow through pores in their pore structure contribute to low flow resistance. The resulted monolithic columns have promising potential for dual mode liquid chromatography. MATE may contribute for anion-exchange while VBC may responsible for reversed-phase liquid chromatography.

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“…Sedangkan kolom berdiameter besar bersifat kurang homogen. Homogenitas yang rendah disebabkan oleh pengaruh pemanasan yang tidak merata dan pengaruh gravitasi pada proses pembentukan dan pengendapan polimer (growing gravitational settling effect) [14][15][16].…”

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Preparasi Kolom Microbore Monolith Berbasis Polistirena untuk Pemisahan Alkilbenzena Menggunakan Kromatografi Cair Kinerja Tinggi

Tasfiyati

Solandjari

Iftitah

et al. 2015

Nat-B

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Kata kunci : kolom monolith, microbore, polistirena, kromatografi cair fasa terbalik, alkilbenzena ABSTRACTIn this study, polystyrene-based monolith was developed as reversed phase column in high performance liquid chromatography (HPLC) for separation of small molecule sample (alkylbenzene). Polystyrene-based monolithic column was prepared in microbore silicosteel column (100 x 1.02 mm i.d.) by in-situ polymerization reaction using styrene as monomer, divinylbenzene as crosslinker, porogenic solvents, with the presence of radical initiator 2,2'-azobis(isobutyronitrile). The morphology of the monolithic column was studied by SEM. The properties of the monolithic column, such as permeability, mechanical stability, binding capacity and pore size distribution, were characterized in detail. From the characterization results of 9 monolith variation, monolith PS-DVB with %T 30, %C 50, using toluene:1-decanol (3:7 v/v) as porogenic solvent was found to be the ideal composition and gave the best separation of alkylbenzene. It has theoretical plate value (N) of 17260/m column, also has relatively equal proportion of flow-through pore and mesopores (47% and 44% respectively). The performance of monolithic column was also compared with commercially available monolithic column Proswift RP-4H. PS-DVB monolith prepared in this study shown its superiority over commercial one.Keywords : monolithic column, microbore, polystyrene, reversed phase liquid chromatography, alkylbenzene PENDAHULUANMonolith adalah material berpori kontinyu dengan struktur pori yang terdiri dari 3 jenis ukuran, yaitu micropore (<2 nm), mesopore (2-

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Preparation and characterization of lauryl methacrylate-based monolithic microbore column for reversed-phase liquid chromatography

Cited by 49 publications

References 43 publications

Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns

Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

Preparasi Kolom Microbore Monolith Berbasis Polistirena untuk Pemisahan Alkilbenzena Menggunakan Kromatografi Cair Kinerja Tinggi

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