Preparation and characterization of interferon‐loaded magnetic biodegradable microspheres

Abstract

In this work, magnetite (Fe(3)O(4)) nanoparticles with an average size 10 nm modified by sodium oleate were prepared by the modified controlled chemical coprecipitation method, which can be well dispersed in water and linked well with protein molecules because of the presence of -COOH on their surface. Then magnetic poly(lactic acid) (PLA) and poly(lactic-co-glycolic acid) (PLGA) microspheres containing interferon alpha-2b (IFN-a-2b) were prepared by the modified water-in-oil-in-water solvent evaporation procedure. X-ray powder diffraction analysis, particle size analysis, transmission electron microscopy, scanning electron microscopy, and vibrating-sample magnetometer (VSM) analysis were carried out to examine phase composition, surface and interior morphology, size and size distribution, and magnetic properties of the magnetic microspheres. Also the effects of some important parameters on the magnetic biodegradable microspheres were investigated, such as magnetite dosage in the preparation system, stirring rate of the suspension medium, and concentration of the external aqueous phase. And the antiviral activity of IFN-a-2b encapsulated in the magnetic polymeric microspheres was evaluated by the vesicular stomatitis virus (VSV) cytopathicity inhibition assay. The results showed that the properties of IFN-loaded magnetic PLGA and PLA microspheres were better than the conventional protein-loaded polymeric microspheres, such as perfect magnetic properties, higher protein encapsulation efficiency, and less effect on the antiviral activity of protein. These indicated that the magnetic PLA and PLGA microspheres containing IFN-a-2b exhibited strong potential as targeted-drug delivery vehicles, which could be rapidly localized to the immunization-related tissues easily by an external magnetic field.

Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy

“…Magnetic microspheres have been used as targeted delivery systems for interferon alpha-2b, protein drugs, 4′-epidoxorubin, doxorubicin, mitoxantrone, mitomycin C, etoposide, paclitaxel, and oxaliplatin. 22,23 In addition, magnetic delivery systems have been used to deliver radioisotopes. Hafeli et al showed that this delivery method improved tumor cell eradication without any harm to normal tissue.…”

Preparation and cellular uptake of PLGA particles loaded with lamivudine

“…Among the method for preparation of PLGA particles, the emulsification-solvent evaporation is the most frequently employed one [19]. The water/oil/water (W/O/W) double emulsion technique has been successfully developed and used to incorporate hydrophilic molecules such as hydrophilic drugs, proteins and nucleotide into the lumen of biodegradable particles [20][21][22][23].…”

Co-encapsulation of human serum albumin and superparamagnetic iron oxide in PLGA nanoparticles: Part I. Effect of process variables on the mean size

“…clustering (Astete et al 2007) which explains the shifting of the mean particle size and size distribution towards the higher values. The latter explanation may be supported by the study Zhou et al (Zhou et al 2008) who studied the size of interferon loaded found that size of both types of microspheres increased and the size distribution broadened with the increasing amounts of magnetic nanoparticles. On the other hand it is also expected that higher number of Fe 3 O 4 nanoparticles inside the PLGA nanoparticles may adsorb more PLGA, which increases the amount of polymer in a particle, increasing its mass and size.…”