Preparation and characterization of high capacity, strong cation-exchange fiber based adsorbents

Schwellenbach, Jan; Taft, Florian; Villain, Louis; Strube, Jochen

doi:10.1016/j.chroma.2016.04.019

Cited by 25 publications

(14 citation statements)

References 51 publications

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“…As estimated before, the thickness of the hydrogel layer using this surface modification technique reaches values >300 nm [ 10 ]. Based on this finding, it can be assumed that the channels formed by the winged-like extensions are completely filled with hydrogel bearing ligand sites.…”

Section: Theoreticalmentioning

confidence: 75%

“…The fibers were surface modified as reported previously [ 10 ]. Briefly, the fiber surface was modified by grafting glycidyl methacrylate (GMA) via surface-initiated atom transfer radical polymerization (SI-ATRP) and a subsequent derivatization leading to sulfonic acid groups.…”

Section: Methodsmentioning

confidence: 99%

“…The preparation of a packed fiber bed was performed as described previously [ 10 ]. Briefly, in a representative packing procedure 0.825 g of PET-SO 3 − fibers were, prior to column packing, slurried in ethanol, filtered off, and dried at 60 °C for 4 h to yield a fiber sample free of agglomerates.…”

Section: Methodsmentioning

confidence: 99%

“…The structure of a packed bed of these fibers has been investigated and discussed in detail before. A bimodal structure within the fiber bed has been revealed, consisting of larger transport channels formed by the voidage between the fibers, and a hydrogel layer with porous properties [ 10 ]. Based on these findings, the fiber bed column is schematically considered as an ideal porous medium of total length L with uniform interstitial voidage

between the fibers and stationary phase distribution.…”

Section: Theoreticalmentioning

confidence: 99%

“…Recent advances in the production of fibers with shaped cross-sections based on extrusion processes offer the possibility to produce high-surface area fibers composed of different thermoplastic base materials [ 9 ]. As reported, a packed bed of these fiber materials, after a suitable surface modification, successfully addresses the shortcomings of hydrogel grafted non-porous adsorption supports regarding their often limited binding capacity, but maintain their advantages such as fast mass-transfer kinetics, high permeability, and accessibility of the binding sites for larger target molecules [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Purification of Monoclonal Antibodies Using a Fiber Based Cation-Exchange Stationary Phase: Parameter Determination and Modeling

et al. 2016

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Monoclonal antibodies (mAb) currently dominate the market for protein therapeutics. Because chromatography unit operations are critical for the purification of therapeutic proteins, the process integration of novel chromatographic stationary phases, driven by the demand for more economic process schemes, is a field of ongoing research. Within this study it was demonstrated that the description and prediction of mAb purification on a novel fiber based cation-exchange stationary phase can be achieved using a physico-chemical model. All relevant mass-transport phenomena during a bind and elute chromatographic cycle, namely convection, axial dispersion, boundary layer mass-transfer, and the salt dependent binding behavior in the fiber bed were described. This work highlights the combination of model adaption, simulation, and experimental parameter determination through separate measurements, correlations, or geometric considerations, independent from the chromatographic cycle. The salt dependent binding behavior of a purified mAb was determined by the measurement of adsorption isotherms using batch adsorption experiments. Utilizing a combination of size exclusion and protein A chromatography as analytic techniques, this approach can be extended to a cell culture broth, describing the salt dependent binding behavior of multiple components. Model testing and validation was performed with experimental bind and elute cycles using purified mAb as well as a clarified cell culture broth. A comparison between model calculations and experimental data showed a good agreement. The influence of the model parameters is discussed in detail.

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Section: Theoreticalmentioning

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

between the fibers and stationary phase distribution.…”

Section: Theoreticalmentioning

confidence: 99%