Preparation and Characterization of Functionalized Graphene Oxide Carrier for siRNA Delivery

Abstract

A successful siRNA delivery system is dependent on the development of a good siRNA carrier. Graphene oxide (GO) has gained great attention as a promising nanocarrier in recent years. It has been reported that GO could be used to deliver a series of drugs including synthetic compounds, proteins, antibodies, and genes. Our previous research indicated that functionalized GO could deliver siRNA into tumor cells and induce a gene silencing effect, to follow up the research, in this research, GO-R8/cRGDfV(GRcR) was designed and prepared for VEGF-siRNA delivery as a novel carrier. The Zeta potential and particle size of the new designed GRcR carrier was measured at (29.46 ± 5.32) mV and (135.7 ± 3.3) nm respectively, and after transfection, the VEGF mRNA level and protein expression level were down-regulated by 48.22% (p < 0.01) and 38.3% (p < 0.01) in HeLa cells, respectively. The fluorescent images of the treated BALB/c nude mice revealed that GRcR/VEGF-siRNA could conduct targeted delivery of VEGF-siRNA into tumor tissues and showed a gene silencing effect as well as a tumor growth inhibitory effect (p < 0.01) in vivo. Further studies showed that GRcR/VEGF-siRNA could effectively inhibit angiogenesis by suppressing VEGF expression. Histology and immunohistochemistry studies demonstrated that GRcR/VEGF-siRNA could inhibit tumor tissue growth effectively and have anti-angiogenesis activity, which was the result of VEGF protein downregulation. Both in vitro and in vivo results demonstrated that GRcR/VEGF-siRNA could be used as an ideal nonviral tumor-targeting vector for VEGF-siRNA delivery in gene therapy.

Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy

“…1 C). In our previous research, GO was decorated with cationic materials such as octaarginine (R8) and poly- L -lysine (PLL) for siRNA delivery [45] , [46] , [47] . The zeta potential of CS functionalized GO was (41.94 ± 0.34) mV ( Fig.…”

Preparation Optimization of Bovine Serum Albumin Nanoparticles and Its Application for siRNA Delivery

“…The expression of survivin-mRNA was measured by RT-qPCR. 32 MCF-7 cells were seed in six-well dishes at the density of 2×10 5 cells/well for 12h. After washing 2 times with PBS, the cells were treated with 100 nM of siRNA, Lipo/NC, BSA NPs/NC, Lipo/siRNA and BSA NPs/siRNA separately for 6 h. Then the solution in dishes was replaced by completed medium, and the cells were incubated for another 42 h. TRIzol reagent was applied to exact the total RNA.…”

Structural characterization, anti-inflammatory and glycosidase inhibitory activities of two new polysaccharides from the root of Pueraria lobata

“…The peak caused by the strong stretching vibration of O–H was observed at 3369.5 cm −1 . 32 The band at 2929.7 cm −1 was ascribed to the C–H stretching vibration. The peak at 1415.5 cm −1 is caused by the C–H stretching vibration.…”