Preparation and characterization of epigallocatechin-3-gallate loaded melanin nanocomposite (EGCG @MNPs) for improved thermal stability, antioxidant and antibacterial activity

Zhao, Wangchen; Liu, Ziyu; Liang, Xiaoyun; Wang, Siqi; Ding, Jie; Li, Zhuozhu; Wang, Longfeng; Jiang, Yun

doi:10.1016/j.lwt.2021.112599

Cited by 26 publications

(17 citation statements)

References 38 publications

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“…Thus, enzymatic browning was also one factor responsible for the formation of BQ. Melanin has many excellent properties, such as strong free radical scavenging ability ( Ma et al, 2018 ), resistance to ultraviolet radiation ( Ye et al, 2019 ), heat resistance ( Li et al, 2019 ), and antibacterial ( Vasanthabharathi et al, 2011 ) and cell protection ( Langfelder et al, 2003 ), and can be used as antioxidants ( Zhao et al, 2022 ), anti-tumor drugs ( Ye et al, 2019 ), and hair dyes ( Battistella et al, 2020 ). Therefore, studies aimed at screening of melanin-producing bacteria from Daqu and identifying the melanin structure and function are needed.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Formation of Sauce-Flavored Daqu Using Non-targeted Metabolomics

Luo

Zhang²,

Yang³

et al. 2022

Front. Microbiol.

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Sauce-flavored Daqu exhibits different colors after being stacked and fermented at high temperatures. Heiqu (black Daqu, BQ) with outstanding functions is difficult to obtain because its formation mechanism is unclear. In this study, we compared the metabolites in different types of Daqu using ultra-high-performance liquid chromatography triple quadrupole mass spectrometry to explore the formation process of BQ. We found that 251 differential metabolites were upregulated in BQ. Metabolic pathway analysis showed that “tyrosine metabolism” was enriched, and most metabolites in this pathway were differential metabolites upregulated in BQ. The tyrosine metabolic pathway is related to enzymatic browning and melanin production. In addition, the high-temperature and high-humidity fermentation environment of sauce-flavored Daqu promoted an increase in the melanoidin content via a typical Maillard reaction; thus, the melanoidin content in BQ was much higher than that in Huangqu and Baiqu. By strengthening the Maillard reaction precursor substances, amino acids, and reducing sugars, the content of Daqu melanoidin increased significantly after simulated fermentation. Therefore, the enzymatic browning product melanin and Maillard reaction product melanoidin are responsible for BQ formation. This study revealed the difference between BQ and other types of Daqu and provides theoretical guidance for controlling the formation of BQ and improving the quality of liquor.

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Section: Discussionmentioning

confidence: 99%

Analysis of the Formation of Sauce-Flavored Daqu Using Non-targeted Metabolomics

Luo

Zhang²,

Yang³

et al. 2022

Front. Microbiol.

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“…Moreover, some new absorptions appear in the spectrum of HH-E. The absorptions at 1691 cm À1 are attributed to the C O in EGCG, the absorptions at 1631 and 1456 cm À1 are involved with the aromatic ring stretching 50 and the absorptions at 1343 cm À1 are attributed to C O of EGCG. 51 According to FT-IR analysis, EGCG is indeed loaded on HH.…”

Section: Characterization Of Antioxidant Loadedmentioning

confidence: 98%

Sustained antioxidant properties of epigallocatechin gallate loaded halloysite for PLA as potentially durable materials

Yao

Gong

et al. 2022

J of Applied Polymer Sci

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Halloysite nanotube (HNT) was etched by hydrochloric acid, and epigallocatechin gallate (EGCG) was loaded into the etched HNT (HH) to prepare loaded antioxidant (HH-E) for polylactic acid (PLA) with improved thermal-oxidative stability. The loading process was confirmed by TGA and FT-IR, and the loading capacity was 16.15%. The presence of EGCG on the surface of etched HNT was further confirmed by nitrogen adsorption and TEM. The effect of loaded EGCG on thermal-oxidative stability of PLA was characterized by the change in OOT values of PLA after the aging process under 140 C. The results showed that HH-E increased OOT values of PLA from 231.2 C to 293.6 C at first, indicating that HH-E provided PLA with excellent thermal-oxidative stability. Furthermore, as can be seen from the trend of OOT changing over aging time, during the 14 days aging process, OOT values of PLA composites with HH-E decreased more slowly than that of PLA composites with EGCG alone, endowing PLA with sustainable thermal-oxidative stability. Further kinetic analysis showed that the apparent activation energy of the degradation process (E a ) of PLA composites with HH-E changed less than that of PLA composites with EGCG alone, which showed that the loading system provided PLA better sustainable stability. Moreover, the isothermal crystallization process of modified PLA was characterized by DSC and POM. The results showed that HH-E provided heterogeneous crystal nucleus for PLA, reducing the half crystallization time (t 0.5 ) and improvement of crystallization rate for PLA. These results prove that HH-E exhibited excellent performance in improving the thermal stability and crystallization property of PLA, showing multifunctional characterization.

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“…Briefly, EGCG was isolated from C. sinensis using a thin-layer chromatography method and verified by a comparison with epigallocatechin gallate hydrate (Tokyo Chemical Co., Ltd., Japan). 13 Subsequently, 5 mL of 0.1% chitosan solution [containing low molecular weight chitosan (Sigma-Aldrich) in 1% acetic acid] was added to 50 mL of EGCG solution [0.05% EGCG (Sigma-Aldrich) in distilled water], and the mixture was stirred at room temperature. Next, 0.5 mL of triphosphate (TPP) solution [0.025% TPP (Merck) in distilled water] was added drop-wise with stirring for 3 h at 112 Â g. This solution was centrifuged at 21,952 Â g for 10 min using a MC-10K centrifuge (Bio-Gener, Hangzhou, China) and washed three times with deionized water to obtain the EGCG CNPs, which were freeze dried for 48 h and stored at 4°C.…”

Section: Preparation Of Egcg Cnpsmentioning

confidence: 99%

Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen

Mustofa

Susilowati²,

Suprayogi³

et al. 2023

F1000Res

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Background and Aim: The Kacang goat (Capra hircus) is an indigenous livestock species in Indonesia that is at risk of extinction due to cross-breeding. Artificial insemination (AI) techniques are expected to increase the population of these goats. This study aimed to determine the addition of epigallocatechin-3-gallate chitosan nanoparticles (EGCG CNPs) to skim milk–egg yolk (SM–EY) extender to obtain the best possible quality of post-thawed Kacang buck semen for AI. Materials and Methods: Fresh Kacang buck semen was diluted in SM–EY without or with the addition of 0.5, 1.0, 1.5, or 2.0 µg of EGCG CNPs/mL extender. Extended semen was packaged in French mini straws, frooze, and stored in liquid nitrogen at −196℃ for 24 hours. Six replicates from each treatment group were thawed for catalase, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, malondialdehyde (MDA), sperm intact plasma membrane (IPM), viability and motility analyses. Results: Post-thawed semen that was previously frozen without EGCG CNPs in the extender (control group) exhibited the lowest levels of catalase, DPPH, sperm viability, sperm motility, IPM, and the highest levels of MDA. However, the addition of EGCG CNPs at doses of 1.5 µg/mL extender increased post-thawed catalase, DPPH, sperm IPM, viability, and sperm motility and decreased MDA levels (p < 0.05) than those of control group. Conclusion: This study was the first in which EGCG CNPs were used in SM–EY extender, and the addition of only 1.0 µg/mL of EGCG CNPs in this extender increased the antioxidant capacity and post-thawed quality of Kacang buck semen.

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Preparation and characterization of epigallocatechin-3-gallate loaded melanin nanocomposite (EGCG @MNPs) for improved thermal stability, antioxidant and antibacterial activity

Cited by 26 publications

References 38 publications

Analysis of the Formation of Sauce-Flavored Daqu Using Non-targeted Metabolomics

Analysis of the Formation of Sauce-Flavored Daqu Using Non-targeted Metabolomics

Sustained antioxidant properties of epigallocatechin gallate loaded halloysite for PLA as potentially durable materials

Epigallocatechin-3-gallate chitosan nanoparticles in an extender improve the antioxidant capacity and post-thawed quality of Kacang goat semen

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