Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering

Hashimoto, Yoshihide; Funamoto, Seiichi; Sasaki, Satoshi; Honda, Toshio; Hattori, Satoshi; Nam, Kwangwoo; Kimura, Tsuyoshi; Mochizuki, Manabu; Fujisato, Toshiya; Kobayashi, Haruo; Kishida, Akio

doi:10.1016/j.biomaterials.2010.01.122

Cited by 224 publications

(195 citation statements)

References 33 publications

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“…49,51-54 To eliminate the toxic effect of chemicals, Hashimoto and coworkers used high-hydrostatic pressurization (HHP) to decellularize porcine corneas, which were then transplanted into rabbit corneas. 55 No immune reaction was observed and the turbid corneas became clear. Lee et al used freezing-thawingcentrifugation of porcine cornea to the remove cells from the cornea while preserving the stromal architecture.…”

Section: Decellularized Corneas As Scaffoldsmentioning

confidence: 97%

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

et al. 2012

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A range of alternatives to human donor tissue for corneal transplantation are being developed to address the shortfall of good quality tissues as well as the clinical conditions for which allografting is contraindicated. Classical keratoprostheses, commonly referred to as artificial corneas, are being used clinically to replace minimal corneal function. However, they are used only as last resorts, as they are associated with significant complications, such as extrusion/rejection, glaucoma, and retinal detachment. The past few years have seen significant developments in technologies designed to replace part or the full thickness of damaged or diseased corneas with materials that encourage regeneration to different extents. This review describes selected examples of these corneal substitutes, which range from cell-based regenerative strategies to keratoprostheses with regenerative capabilities via tissue-engineered scaffolds pre-seeded with stem cells. It is unlikely that one corneal substitute will be best for all indications, but taken together, the various approaches may soon be able to supplement the supply of human donor corneas for transplantation or allow restoration of diseased or damaged corneas that cannot be treated by currently available techniques.

Funding Agencies|Swedish Research Council, County Council of Ostergotland, Sweden||Canadian Stem Cell Network||NSERC Canada|SAF2011-22500|Spanish Ministerio de Economia y Competitividad||

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Section: Decellularized Corneas As Scaffoldsmentioning

confidence: 97%

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

et al. 2012

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Funding Agencies|Swedish Research Council, County Council of Ostergotland, Sweden||Canadian Stem Cell Network||NSERC Canada|SAF2011-22500|Spanish Ministerio de Economia y Competitividad||

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“…This approach to biologic scaffold generation has been successful with a variety of tissue types and the resultant acellular ECM has been examined in both pre-clinical studies and human clinical applications (22,(40)(41)(42). One problem associated with corneal decellularization is control of tissue hydration (43)(44)(45). During an effective decellularization process the endothelium of the cornea is completely removed.…”

Section: Discussionmentioning

confidence: 99%

Dextran Preserves Native Corneal Structure During Decellularization

Lynch

Wilson

Ahearne

2016

Tissue Engineering Part C: Methods

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“…Recently, several groups have succeeded in preparing an acellular corneal stroma by using non-ionic detergents and/or enzymes. Hashimoto et al used high-hydrostatic pressurization (HHP) to decellularize porcine corneas, which were then transplanted into rabbit corneas (2). No immune reaction was observed, and the turbid corneas became clear.…”

Section: Introductionmentioning

confidence: 99%

Lamellar Keratoplasty Treatment of Fungal Corneal Ulcers With Acellular Porcine Corneal Stroma

Zhang

Liu

Jin

et al. 2015

American Journal of Transplantation

130

115

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Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering

Cited by 224 publications

References 33 publications

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

Dextran Preserves Native Corneal Structure During Decellularization

Lamellar Keratoplasty Treatment of Fungal Corneal Ulcers With Acellular Porcine Corneal Stroma

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