Preparation and characterization of chitosan/graphene oxide composite hydrogels for nerve tissue Engineering

Jafarkhani, Mahboubeh; Salehi, Zeinab; Nematian, Tahereh

doi:10.1016/j.matpr.2018.04.171

Cited by 25 publications

(12 citation statements)

References 21 publications

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“…Although the presented mechanical properties did not precisely match with those of the common neural tissue, nevertheless, this research evidence did not preclude the effectiveness of the presented structures for neural tissue regeneration as demonstrated by others in literature. 35 The morphological characterization of the G-CB-GPTMS samples via SEM confirmed all the deduction about the mechanical and swelling properties of hydrogels. Further, SEM images demonstrated that the concentration of 0.9 % of CB was too high to guarantee a uniform dispersion of the filling agent inside the gelatin matrix, causing a degradation of both mechanical and swelling properties.…”

Section: Discussionsupporting

confidence: 70%

“…34 Despite this aspect, it cannot be ruled out that the presented G-CB-GPTMS structure could support neural growth as proved by others in literature. 35 In this regard, as a second stage of this work, the response of neural cells to these structures will be evaluated to validate whether there is an effect of interest to pursue this material for its final application or if a modulation of the composition of the structures will be needed in favor of structures with improved mechanical properties.…”

Section: Mechanical Characterizationmentioning

confidence: 99%

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Fabrication and Characterization of Gelatin/Carbon Black–Based Scaffolds for Neural Tissue Engineering Applications

Gunasekaran

Acutis

Montemurro

et al. 2019

Materials Performance and Characterization

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Neural tissue engineering has recently emerged as an alternative strategy to repair nerve damage and promote nerve regeneration. It involves the fabrication of scaffolds with properties mimicking those of the natural extracellular matrix for guiding a three-dimensional (3D) neural regeneration.These engineered constructs, in addition to mechanical support, should be also capable of providing proper chemical and electrical stimuli for adhesion, migration, and proliferation of the neural cells. In this study, we developed conductive composite hydrogel films based on gelatin and carbon black (CB) as scaffolds for neural tissue engineering applications. The presented hydrogel constructs were fabricated in the form of films using the solvent casting method after dispersing several concentrations of CB in a 5 % (w/v) gelatin solution along with (3-glycidoxypropyl) trimethoxysilane (GPTMS) as the crosslinking agent at a concentration of 1.84 % (v/v). The CB concentrations of 0.3 %, 0.5 %, 0.7 %, and 0.9 % (w/w) with respect to the gelatin amount were chosen. The morphological, compositional, swelling, dissolution, electrical, mechanical, and wettability properties together were characterized as function of CB content and compared with those of pure gelatin-based hydrogel. Results demonstrated that the incorporation of different quantities of CB relatively reduced the water uptake capability of the films and increased the stability in water of the gelatin matrix. Findings from the mechanical tests revealed that composite hydrogels have a lower elastic modulus with respect to the pure gelatin matrix. Moreover, it was found that the incorporation of incremental CB concentrations kept the wettability surface property unchanged while the electrical characterization of the proposed structures showed a reduction of the electrical impedance. Overall, the study suggests that the composite structures could be used as a potential candidate for fabrication of scaffolds for neural regeneration with tunable electrical and mechanical properties by varying the CB concentration in a finite range.

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Section: Discussionsupporting

confidence: 70%

Section: Mechanical Characterizationmentioning

confidence: 99%

Fabrication and Characterization of Gelatin/Carbon Black–Based Scaffolds for Neural Tissue Engineering Applications

Gunasekaran

Acutis

Montemurro

et al. 2019

Materials Performance and Characterization

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“…Graphene also has the ability to interact with tissues, biomolecules and cells which adds another application of GO in various biomedical fields such as drug delivery and tissue engineering [ 17 ]. Nanographene oxide/chitosan influences the adhesion and proliferation rate of nerve cells with a 20% increase in nerve cell growth [ 19 ]. CS/GO nanocomposite membrane has improved in congo red dye absorption uptake with >90% removal efficiency [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation on Structural Properties and Performances of Graphene Oxide Incorporated into Chitosan/Poly-Lactic Acid Composites: CS/PLA versus CS/PLA-GO

et al. 2021

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In this work, to fabricate a novel composite consisting of chitosan/poly-lactic acid doped with graphene oxide (CS/PLA-GO), composites were prepared via solution blending method to create various compositions of CS and PLA (90/10, 70/30 and 50/50CS/PLA-GO). Graphene oxide (GO) was added into a PLA solution prior to blending it with chitosan (CS). The surface morphology and structural properties of synthesized composites were characterized using FT-IR, SEM and XRD analysis. The performances of synthesized composites on thermal strength, mechanical strength, water absorption, and microbial activity were also evaluated through standard testing methods. The morphology of 70/30CS/PLA-GO became smoother with the addition of GO due to enhanced interfacial adhesion between CS, PLA and GO. The presence of GO has also improved the miscibility of CS and PLA and has superior properties compared to CS/PLA composites. Moreover, the addition of GO has boosted the thermal stability of the composite, with a significant enhancement of Td and Tg. The highest Td and Tg were accomplished at 389 °C and 76.88 °C, respectively, for the 70/30CS/PLA-GO composite in comparison to the CS and PLA that recorded Td at 272 °C and 325 °C and Tg at 61 °C and 60 °C, respectively. In addition, as reinforcement, GO provided a significant influence on the tensile strength of composites where the tensile modulus showed remarkable improvement compared to pure CS and CS/PLA composites. Furthermore, CS/PLA-GO composites showed excellent water-barrier properties. Among other compositions, 70/30CS/PLA revealed the greatest decrement in water absorption. From the antibacterial results, it was observed that 90/10CS/PLA-GO and 70/30CS/PLA-GO showed an inhibitory effect and had wide inhibition zones which were 8.0 and 8.5 mm, respectively, against bacteria Bacillus Subtillis B29.

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“…Some other functional materials are also used to manufacture composite hydrogels. Jafarkhani et al [103] synthesized nano graphene oxide/chitosan (NGO/CHT) composite hydrogels, the NGO addition changed the pore structure and improved mechanical strength of hydrogel and increased nerve cells growth up to 20%. More examples of conductive composite hydrogels are introduced in Section 4.3.1.…”

Section: Composite Hydrogelsmentioning

confidence: 99%

3D Printing and Bioprinting Nerve Conduits for Neural Tissue Engineering

Zhang

2020

Polymers

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Fabrication of nerve conduits for perfectly repairing or replacing damaged peripheral nerve is an urgent demand worldwide, but it is also a formidable clinical challenge. In the last decade, with the rapid development of manufacture technologies, 3D printing and bioprinting have been becoming remarkable stars in the field of neural engineering. In this review, we explore that the biomaterial inks (hydrogels, thermoplastic, and thermoset polyesters and composite) and bioinks have been selected for 3D printing and bioprinting of peripheral nerve conduits. This review covers 3D manufacturing technologies, including extrusion printing, inkjet printing, stereolithography, and bioprinting with inclusion of cells, bioactive molecules, and drugs. Finally, an outlook on the future directions of 3D printing and 4D printing in customizable nerve therapies is presented.

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Preparation and characterization of chitosan/graphene oxide composite hydrogels for nerve tissue Engineering

Cited by 25 publications

References 21 publications

Fabrication and Characterization of Gelatin/Carbon Black–Based Scaffolds for Neural Tissue Engineering Applications

Fabrication and Characterization of Gelatin/Carbon Black–Based Scaffolds for Neural Tissue Engineering Applications

Evaluation on Structural Properties and Performances of Graphene Oxide Incorporated into Chitosan/Poly-Lactic Acid Composites: CS/PLA versus CS/PLA-GO

3D Printing and Bioprinting Nerve Conduits for Neural Tissue Engineering

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