Preparation and characterization of cationic Pluronic for surface modification and functionalization of polymeric drug delivery nanoparticles

Gyulai, Gergö; Magyar, Anna; Rohonczy, János; Orosz, J.; Yamasaki, Marcelo M.; Bősze, Sz.

doi:10.3144/expresspolymlett.2016.20

Cited by 47 publications

(29 citation statements)

References 50 publications

(36 reference statements)

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“…PLGA nanoparticles with a narrow size distribution were prepared using the nanoprecipitation method as described previously [26,27]. Briefly PLGA was dissolved in acetone at a concentration of 10 g/L.…”

Section: Preparation and Characterization Of Plga Nanoparticlesmentioning

confidence: 99%

Interaction of poly(lactic-co-glycolic acid) nanoparticles at fluid interfaces

Gyulai

Kiss

2017

Journal of Colloid and Interface Science

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Interfacial adsorption and its kinetics were explained by the diffusion controlled adsorption theory and considering the energy barrier of particle transport to the interface. Surface modification by Pluronic increased the interfacial activity of nanoparticles at all interfaces. Surface activity of PLGA-Pluronic particles could be described by the contributions of both the PLGA NPs and the effective portion of their Pluronic shell. Both particle films present mainly elastic dilatational properties suggesting that particles are in kinetically separated state.

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Section: Preparation and Characterization Of Plga Nanoparticlesmentioning

confidence: 99%

Interaction of poly(lactic-co-glycolic acid) nanoparticles at fluid interfaces

Gyulai

Kiss

2017

Journal of Colloid and Interface Science

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“…Following immersion, catheter segments were recovered and washed twice with PBS to remove the unadsorbed polymer. All the selected P388 concentrations were lower than polymer critical micelle concentration, which is 33.4 g/L at 25 • C [37].…”

Section: P388 Adsorption Onto Silicone Tubesmentioning

confidence: 90%

Poloxamer 338 Affects Cell Adhesion and Biofilm Formation in Escherichia coli: Potential Applications in the Management of Catheter-Associated Urinary Tract Infections

et al. 2020

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Poloxamers are nontoxic, amphiphilic copolymers used in different formulations. Due to its surfactant properties, Poloxamer 338 (P388) is herein proposed as a strategy to avoid biofilm formation often causing recalcitrant catheter-associated urinary tract infections (CAUTI). The aim is to evaluate the ability of P388 coatings to affect the adhesion of Ec5FSL and Ec9FSL Escherichia coli strains on silicone urinary catheters. Attenuated total reflection infrared spectroscopy, atomic force microscopy, and static water contact angle measurement were employed to characterize the P388-coated silicone catheter in terms of amount of P388 layered, coating thickness, homogeneity, and hydrophilicity. In static conditions, the antifouling power of P388 was defined by comparing the E. coli cells adherent on a hydrophilic P388-adsorbed catheter segment with those on an uncoated one. A P388-coated catheter, having a homogeneous coverage of 35 nm in thickness, reduced of 0.83 log10 and 0.51 log10 the biofilm of Ec5FSL and Ec9FSL, respectively. In dynamic conditions, the percentage of cell adhesion on P388-adsorbed silicone channels was investigated by a microfluidic system, simulating the in vivo conditions of catheterized patients. As a result, both E. coli isolates were undetected. The strong and stable antifouling property against E. coli biofilm lead us to consider P388 as a promising anti-biofilm agent for CAUTIs control.

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“…Pluronic block copolymers have been used and applied as a micellar nano-carrier for drug delivery system, resulting in increased solubility and metabolic stability, and improving circulation time. Gyulai et al reported that the presence of pluronics on the surface of poly lactic-co-glycolic acid (PLGA) nanoparticles preserved the aggregation stability and improved the nonspecific membrane affinity of the system [17].…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Hemoglobin-Based Oxygen Carriers Reduces Recognition by Haptoglobin, Immunoglobulin, and Hemoglobin Antibodies

et al. 2019

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Hemoglobin-based oxygen carriers (HBOCs) represent a propitious type of blood substitute to transport oxygen throughout the body while acting as a carrier in biomedical applications. However, HBOCs in blood are recognized and rapidly scavenged by the body’s innate immune systems. To overcome this problem, HBOCs require a surface modification that provides protection against detection and elimination in order to prolong their circulation time after administration. In this study, we investigated different surface modifications of hemoglobin submicron particles (HbMPs) by double/triple precipitation, as well as by adsorption of human serum albumin (HSA), hyaluronic acid (HA), and pluronic (Plu) to discover how diverse surface modifications influence the oxygen binding capacity and the binding of anti-hemoglobin (Hb) antibodies, immunoglobulin G (IgG), and haptoglobin (HP) to HbMPs. The particle size and zeta potential of the six types of HbMP modifications were analyzed by zeta sizer, confocal laser scanning microscopy, and transmission electron microscopy (TEM), and were compared to the unmodified HbMPs. The results revealed that all surface-modified HbMPs had a submicron size with a negative charge. A slight decrease in the oxygen binding capacity was noticed. The specific binding of anti-Hb antibodies, IgG, and HP to all surface-modified HbMPs was reduced. This indicates a coating design able to protect the particles from detection and elimination processes by the immune system, and should lead to a delayed clearance and the required and essential increase in half-life in circulation of these particles in order to fulfill their purpose. Our surface modification method reflects a promising strategy for submicron particle design, and can lead the way toward novel biomedical applications.

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Preparation and characterization of cationic Pluronic for surface modification and functionalization of polymeric drug delivery nanoparticles

Cited by 47 publications

References 50 publications

Interaction of poly(lactic-co-glycolic acid) nanoparticles at fluid interfaces

Interaction of poly(lactic-co-glycolic acid) nanoparticles at fluid interfaces

Poloxamer 338 Affects Cell Adhesion and Biofilm Formation in Escherichia coli: Potential Applications in the Management of Catheter-Associated Urinary Tract Infections

Surface Modification of Hemoglobin-Based Oxygen Carriers Reduces Recognition by Haptoglobin, Immunoglobulin, and Hemoglobin Antibodies

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