Preparation and characterization of bioadhesive system containing hypericin for local photodynamic therapy

Borghi-Pangoni, Fernanda Belincanta; Junqueira, Mariana Volpato; Ferreira, Sabrina Barbosa de Souza; Silva, Larissa Lachi; Rabello, Bruno Ribeiro; Castro, Lidiane Vizioli de; Baesso, Mauro Luciano; Diniz, Andréa; Caetano, Wilker; Bruschi, Marcos Luciano

doi:10.1016/j.pdpdt.2017.06.016

Cited by 34 publications

(20 citation statements)

References 74 publications

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“…The incorporation of CUR significantly decreased ( p < 0.05) the hardness and compressibility, which is a desirable characteristic to facilitate application of the formulation in the buccal cavity. This behavior has been observed previously for polymer blends containing P407 and other acrylic acid derivative with a similar cross-linking degree as C974P to carry hypericin [69]. In this study, the polymeric micelle relaxation due to the hydrophobic drug could explain this behavior and be related to the low yield values in the flow rheology analysis.…”

Section: Resultssupporting

confidence: 79%

“…On the other hand, CUR did not significantly ( p = 0.06597) influence the adhesiveness values of different preparations. This phenomenon has been observed for other polymer blends containing hydrophobic drugs [7] since the mucoadhesive properties are obtained by the interaction between the hydrophilic portion of the polymeric micelles and mucosa [70].…”

Section: Resultsmentioning

confidence: 64%

“…Photoacoustic spectroscopy is useful in the investigation of permeation and distribution of substances in biological tissues in vitro, ex vivo and in vivo. This technique is based on the determination of optical spectral absorption by a photoacoustic signal created by the interaction of matter with radiation of a known wavelength [7,85]. Besides, the relatively low cost, non-destructible manner, and ability to detect low amounts of sample are interesting for investigation of opaque samples [7].…”

Section: Resultsmentioning

confidence: 99%

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Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

Ferreira¹,

Braga²,

Oliveira³

et al. 2019

Beilstein J. Nanotechnol.

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Mucoadhesive nanostructured systems comprising poloxamer 407 and Carbopol 974P® have already demonstrated good mucoadhesion, as well as improved mechanical and rheological properties. Curcumin displays excellent biological activity, mainly in oral squamous cancer; however, its physicochemical characteristics hinder its application. Therefore, the aim of this study was to develop nanostructured formulations containing curcumin for oral cancer therapy. The photophysical interactions between curcumin and the formulations were elucidated by incorporation kinetics and location studies. They revealed that the drug was quickly incorporated and located in the hydrophobic portion of nanometer-sized polymeric micelles. Moreover, the systems displayed plastic behavior with rheopexy characteristics at 37 °C, viscoelastic properties and a gelation temperature of 36 °C, which ensures increased retention after application in the oral cavity. The mucoadhesion results confirmed the previous findings with the nanostructured systems showing a residence time of 20 min in porcine oral mucosa under flow system conditions. Curcumin was released after 8 h and could permeate through the porcine oral mucosa. Cytotoxicity testing revealed that the formulations were selective to cancer cells over healthy cells. Therefore, these systems could improve the physicochemical characteristics of curcumin by providing improved release and permeation, while selectivity targeting cancer cells.

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Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

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Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

Ferreira¹,

Braga²,

Oliveira³

et al. 2019

Beilstein J. Nanotechnol.

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“…The therapeutic effectiveness of medicines applied topically is related, among other factors, to their diffusion rate ( Sugiura et al, 2014 ). PAS has been applied successfully in the evaluation of the penetration of medicines in non-homogeneous and opaque samples, such as the skin ( Ames et al, 2017 ; Borghi-Pangoni et al, 2017 ), tooth ( Occhi-Alexandre et al, 2018 ), and nail ( Nuglisch et al, 2005 ). In the present study, PAS efficiently showed that PE was able to reach the deep layers of the nail by itself, which illustrates an additional property of this natural compound.…”

Section: Discussionmentioning

confidence: 99%

“…The PAS analysis was performed 24 h after the application. The measurements were obtained with a simple experimental arrangement ( Ames et al, 2017 ; Borghi-Pangoni et al, 2017 ), using the photoacoustic cell illustrated in Figure 1 . The photoacoustic spectra were obtained in the ultraviolet and visible spectrum regions, with a wavelength range of 250–800 nm, the power of the source was 800 W and the light modulation frequency was 13 Hz.…”

Section: Methodsmentioning

confidence: 99%

Propolis Extract for Onychomycosis Topical Treatment: From Bench to Clinic

et al. 2018

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Onychomycosis is a chronic fungal infection of nails, commonly caused by dermatophyte fungi, primarily species of Trichophyton. Because of the limited drug arsenal available to treat general fungal infections and the frequent failure of onychomycosis treatment, the search for new therapeutic sources is essential, and topical treatment with natural products for onychomycosis has been encouraged. Propolis, an adhesive resinous compound produced by honeybees (Apis mellifera), has shown multiple biological properties including significant antifungal and anti-biofilm activities in vitro. In spite of promising in vitro results, in vivo results have not been reported so far. This study assessed an ethanol propolis extract (PE) as a topical therapeutic option for onychomycosis, including its characterization in vitro and its applicability as a treatment for onychomycosis (from bench to clinic). The in vitro evaluation included analysis of the cytotoxicity and the antifungal activity against the planktonic cells and biofilm formed by Trichophyton spp. We also evaluated the capacity of PE to penetrate human nails. Patients with onychomycosis received topical PE treatments, with a 6-month follow-up period. The results of the in vitro assays showed that PE was non-toxic to the cell lines tested, and efficient against both the planktonic cells and the biofilm formed by Trichophyton spp. The results also showed that PE is able to penetrate the human nail. The results for PE applied topically to treat onychomycosis were promising, with complete mycological and clinical cure of onychomycosis in 56.25% of the patients. PE is an inexpensive commercially available option, easy to obtain and monitor. Our results indicated that PE is a promising natural compound for onychomycosis treatment, due to its ability to penetrate the nail without cytotoxicity, and its good antifungal performance against species such as Trichophyton spp. that are resistant to conventional antifungals, both in vitro and in patients.

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