Preparation and characterization of a novel antibacterial hydrogel based on thiolated ovalbumin/gelatin with silver ions

Chen, Yue; Qiu, Ning; Ma, Bin; Hu, Gan; Oh, Deog‐Hwan; Fu, Xing

doi:10.1016/j.ifset.2022.103007

Cited by 15 publications

(8 citation statements)

References 48 publications

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“…Regarding the crosslinking strategy, Traut's reagent‐based modification has been employed in the thiolation of gelatin and proteins [ , 32 ] such as ovalbumin, [ 32 ] human decellularized extracellular matrix (human dECM), [ 20b ] and BSA. [ 5b,8a ] Due to the strong nucleophilic nature of thiol groups, thiolated proteins commonly undergo self‐crosslinking through disulfide bonds [ 20b ] or with the assistance of Ag + .…”

Section: Discussionmentioning

confidence: 99%

Thiolation‐Based Protein–Protein Hydrogels for Improved Wound Healing

Liu,

Guo,

Wang

et al. 2024

Adv Healthcare Materials

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The limitations of protein‐based hydrogels, including their insufficient mechanical properties and restricted biological functions, arise from the highly specific functions of proteins as natural building blocks. A potential solution to overcome these shortcomings is the development of protein‐protein hydrogels, which integrate structural and functional proteins. In this study, we introduce a protein‐protein hydrogel formed by crosslinking bovine serum albumin (BSA) and a genetically engineered intrinsically disordered collagen‐like protein (CLP) through Ag‐S bonding. Our approach involves thiolating lysine residues of BSA and crosslinking CLP with Ag+ ions, utilizing thiolation of BSA and the free‐cysteines of CLP. The resulting protein‐protein hydrogels exhibit exceptional properties, including notable plasticity, inherent self‐healing capabilities, and gel‐sol transition in response to redox conditions. In comparison to standalone BSA hydrogels, these protein‐protein hydrogels demonstrate enhanced cellular viability, and improved cellular migration. In vivo experiments provide conclusive evidence of accelerated wound healing, observed not only in murine models with streptozotocin (Step)‐induced diabetes but also in zebrafish models subjected to UV‐burn injuries. Detailed mechanistic insights, combined with assessments of pro‐inflammatory cytokines and the expression of epidermal differentiation‐related proteins, robustly validate the protein‐protein hydrogel's effectiveness in promoting wound repair.This article is protected by copyright. All rights reserved

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Section: Discussionmentioning

confidence: 99%

Thiolation‐Based Protein–Protein Hydrogels for Improved Wound Healing

Liu,

Guo,

Wang

et al. 2024

Adv Healthcare Materials

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“…F Regarding the crosslinking strategy, Traut's reagent-based modification has been employed in the thiolation of gelatin and proteins [43], such as ovalbumin [43], human decellularized extracellular matrix (human dECM) [29] and BSA [14,17]. Due to the strong nucleophilic nature of thiol groups, thiolated proteins commonly undergo self-crosslinking through disulfide bonds [29] or with the assistance of Ag + [14,17] .…”

Section: Discussionmentioning

confidence: 99%

Thiolation-Based Protein-Protein Hydrogels for Improved Wound Healing

Liu,

Wang,

Guo

et al. 2023

Preprint

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The limitations of protein-based hydrogels, including their insufficient mechanical properties and restricted biological functions, arise from the highly specific functions of proteins as natural building blocks. A potential solution to overcome these shortcomings is the development of protein-protein hydrogels, which integrate structural and functional proteins. In this study, we introduce a protein-protein hydrogel formed by crosslinking bovine serum albumin (BSA) and a genetically engineered intrinsically disordered collagen-like protein (CLP) through Ag-S bonding. Our approach involves thiolating lysine residues of BSA and crosslinking CLP with Ag+ ions, utilizing thiolation of BSA and the free-cysteines of CLP. The resulting protein-protein hydrogels exhibit exceptional properties, including notable plasticity, inherent self-healing capabilities, and gel-sol transition in response to redox conditions. Furthermore, in comparison to standalone BSA hydrogels, these protein-protein hydrogels demonstrate remarkable antibacterial properties, enhanced cellular viability, and improved cellular migration. In vivo experiments provide conclusive evidence of accelerated wound healing, observed not only in murine models with streptozotocin (Step)-induced diabetes but also in zebrafish models subjected to UV-burn injuries. Detailed mechanistic insights, combined with assessments of pro-inflammatory cytokines and the expression of epidermal differentiation-related proteins, robustly validate the protein-protein hydrogel's effectiveness in promoting wound repair. This pioneering approach advances the development of protein-protein hydrogels and serves as a reference for the creation of multifunctional protein-based hydrogels.

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“…[15] Our previous research found that increasing the sulfhydryl content through chemical modification resulted in extreme coordination between the sulfhydryl anion and silver ions, forming solid and reversible ─SAg bonds. [16] Protein-based hydrogels exhibit poor intrinsic antimicrobial properties when used alone in wound healing. Silver ions possess a broad antibacterial spectrum and exhibit potent and longlasting efficacy without inducing toxicity.…”

Section: Introductionmentioning

confidence: 99%

“…[17][18][19] Silver ions can enable a desirable slow-release pattern when integrated into protein-based hydrogels. [16] With favorable biosafety assessment, the resulting silver-containing hydrogels exerted significant inhibitory effects on E. coli and S. aureus. In addition, silver ions acted as crosslinking agents to facilitate gel formation.…”

Section: Introductionmentioning

confidence: 99%

A Novel Food Bore Protein Hydrogel with Silver Ions for Promoting Burn Wound Healing

Fu,

Hu,

Abker

et al. 2024

Macromolecular Bioscience

Self Cite

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Hydrogels have emerged as a promising option for treating local scald wounds due to their unique physical and chemical properties. This study aimed to evaluate the efficacy of ovalbumin/gelatin composite hydrogels in repairing deep II‐degree scald wounds using a mouse dorsal skin model. Trauma tissues collected at various time points were analyzed for total protein content, hydroxyproline content, histological features, and expression of relevant markers. The results revealed that the hydrogel accelerated the healing process of scalded wounds, which was 17.27% higher than the control group. The hydrogel treatment also effectively prevented wound enlargement and redness of the edges caused by infection during the initial stage of scalding. The total protein and hydroxyproline content of the treated wounds were significantly elevated. Additionally, the hydrogel up‐regulated the expression of VEGF (a crucial angiogenic factor) and down‐regulated CD68 (a macrophage marker). In summary, this study provided valuable insights into the potential of multifunctional protein‐based hydrogels in wound healing.This article is protected by copyright. All rights reserved

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Preparation and characterization of a novel antibacterial hydrogel based on thiolated ovalbumin/gelatin with silver ions

Cited by 15 publications

References 48 publications

Thiolation‐Based Protein–Protein Hydrogels for Improved Wound Healing

Thiolation‐Based Protein–Protein Hydrogels for Improved Wound Healing

Thiolation-Based Protein-Protein Hydrogels for Improved Wound Healing

A Novel Food Bore Protein Hydrogel with Silver Ions for Promoting Burn Wound Healing

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