2019
DOI: 10.3390/bios9020060
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Preparation and Characterization of a Pectin Membrane-Based Optical pH Sensor for Fish Freshness Monitoring

Abstract: In a simple and instant procedure for detecting fish freshness, a hydrogel and hydrophilic pectin matrix membrane was used successfully as an optical pH sensor by immobilizing the chromoionophore ETH 5294 (CI), which is very selective and sensitive for the membrane. The Pe/CI optical pH sensor exhibited excellent linearity between pH 5 and pH 9, with a sensor response time of 5 min and reproducibility of 1.49% relative standard deviation (RSD). The sensor showed response stability for 15 days and a response re… Show more

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Cited by 43 publications
(43 citation statements)
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“…The surface area of a solid catalyst has a direct impact on its catalytic activity [37,38]. Hydration and dehydration (recalcination) treatments play a vital role for improving the surface area of a catalyst [39].…”
Section: Braunaeur-emmet-teller (Bet)mentioning
confidence: 99%
“…The surface area of a solid catalyst has a direct impact on its catalytic activity [37,38]. Hydration and dehydration (recalcination) treatments play a vital role for improving the surface area of a catalyst [39].…”
Section: Braunaeur-emmet-teller (Bet)mentioning
confidence: 99%
“…The response of the pH optical sensor at pH 5-9 has a sensitivity of 0.056 (R 2 = 0.0986), where the color change of CI takes place against the change of pH, as reported by Hasanah et al [33]. This becomes the basis for TG determination of this optical biosensor, as a pH change will occur when lipase hydrolyzes TPs into fatty acids.…”
Section: The Response Of a Triglyceride Optical Sensor Using A Pectinmentioning
confidence: 53%
“…Table 2 shows an increase of the biosensor response at pH 6-7, but a decrease at pH 7.5 and 8. The decrease in biosensor sensitivity is caused by the change in the conformation of the enzyme binding sites, thus decreasing their function in degrading triglycerides [33]. In this study, the best sensitivity was given by pH 7-hence, pH 7 is used for the upcoming characterization of the TG biosensor.…”
Section: The Effect Of Buffer Concentration Ph and Loading Enzyme Cmentioning
confidence: 88%
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