Preparation and characterization of 3D human glioblastoma spheroids using an N-octanoyl glycol chitosan hydrogel

Bae, Yoonhee; Joo, Chanyang; Park, Kyoung Hwan; Kang, Sun-Woong; Huh, Kang Moo; Choi, Ji Soo

doi:10.1016/j.ijbiomac.2021.06.083

Cited by 6 publications

(6 citation statements)

References 36 publications

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“…The hydrophilic interface could hinder protein adsorption to weaken the interaction between cells and microgels, thus contributing to the formation of cell–cell interaction and rASC aggregates . Therefore, the hydrophilicity of microgels was studied by calculating the swelling ratio.…”

Section: Resultsmentioning

confidence: 99%

“…The hydrophilic interface could hinder protein adsorption to weaken the interaction between cells and microgels, thus contributing to the formation of cell−cell interaction and rASC aggregates. 26 Therefore, the hydrophilicity of microgels was studied by calculating the swelling ratio. As shown in Figure 2D, the The quantitative DNA contents analysis showed that rASCs did not proliferate in the microgels (Figure S1), indicating that the rASCs did not adhere on the microgels and interactions between microgels and rASCs were weak.…”

Section: Characterization Of the Chondral Microtissuesmentioning

confidence: 99%

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Preparation of Assemblable Chondral and Subchondral Bone Microtissues for Osteochondral Tissue Engineering

Xia

Yan

et al. 2022

ACS Appl. Mater. Interfaces

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Microtissues exhibit great advantages in injecting with minimum invasiveness, mimicking natural tissues, and promoting tissue regeneration. However, very few studies have focused on the construction of osteochondral microtissues that could simultaneously support hyaline-like cartilage and bone tissue regeneration. In this study, chondral microtissues that could favor the formation of hyaline-like cartilages and subchondral bone microtissues that could repair subchondral defects to support the neo-generated cartilages were successfully constructed for osteochondral tissue engineering. For chondral repair, the developed chondral microgels with high porosity and hydrophilicity could make cells spherical, favor the formation of cell aggregates, and show an excellent differentiation effect toward hyaline-like cartilage, thus contributing to the production of chondral microtissues. For subchondral bone repair, the fabricated subchondral microgels realize cell adhesion and proliferation and support the osteogenic differentiation of stem cells, thus favoring the formation of subchondral bone microtissues. The injectable chondral and subchondral bone microtissues could be stably assembled by Michael addition reaction between sulfhydryl groups of microtissues and double bonds of hydrophilic macromolecular cross-linker. At 12 weeks postimplantation, osteochondral microtissues could support the reconstruction of osteochondral-like tissues. The present study provides new insight into the microtissues for repair of osteochondral tissues.

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Section: Resultsmentioning

confidence: 99%

Section: Characterization Of the Chondral Microtissuesmentioning

confidence: 99%

Preparation of Assemblable Chondral and Subchondral Bone Microtissues for Osteochondral Tissue Engineering

Xia

Yan

et al. 2022

ACS Appl. Mater. Interfaces

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“…It is better for studying oncolytic drug effects than regular culture, as it provides an environment for investigating drug effects. In vitro 3D studies of GBM combination therapy showed prominent apoptosis [46,50].…”

Section: Discussionmentioning

confidence: 99%

Comparison of 3D and 2D cultures for evaluating the effect of combination therapy with Erlotinib and Imatinib on Glioblastoma multiforme

Rashidi

Hadjighassem

Negahdari

2023

Preprint

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Purpose Glioblastoma multiforme (GBM) is a common brain tumor with aggressive phenotypes and resistance to treatments. Erlotinib and Imatinib, combined, hold promise as innovative therapies to overcome GBM's heterogeneous malignancy. This study conducted experiments in two-dimensional (2D) and three-dimensional (3D) cell cultures, comparing them to the in vivo microenvironment. Methods The study measured the effects of Imatinib and Erlotinib on the survival of 2D and 3D cell cultures and apoptosis after 24 and 48 hours (h) post-treatment using MTT assay and flow cytometry. The study also investigated gene expression of Bcl-2 and VEGF using qRT-PCR. The scratch test was used to explore drug treatments' effects on cellular migration. Results Based on our results, combination therapy reduced tumor cell migration by reducing Bcl-2 and VEGF gene expression through Erlotinib, Imatinib, or combination therapy. However, combination therapy showed a more significant reduction in B-cl2 gene expression than single therapy, as opposed to VEGF expression. The apoptotic effect of combination therapy was confirmed, with an increase in death percentage in the 48h-3D treated group (*P < 0.05 for monotherapy and ***P < 0.001 for combination). Conclusion 3D culture offers a more physiological environment for studying oncolytic drug effects. In a 3D in vitro study, combination GBM therapy caused significant apoptosis, requiring further preclinical model studies.

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“… 268 Based on these experiments, carbon‐based NPs can provide delivery of DOX in cancer therapy and CS modification improves their beneficial features. 99 , 269 , 270 , 271 , 272 , 273 , 274 , 275 Figure 7 demonstrates modification of carbon‐based nanomaterials with CS and then, conjugation of folic acid as ligand on nanocarriers to mediate their internalization in cervical cancer cells via endocytosis, resulting in a significant increase in accumulation of DOX in tumor cells. 253 , 276 , 277 Table 3 provides a summary of various nanoparticles modified by CS for purpose of DOX delivery in cancer suppression.…”

Section: Nanostructures Of Chitosan‐doxorubicinmentioning

confidence: 99%

“…263,264 For improving the biodegradability of CS and its solubil- CS modification improves their beneficial features. 99,[269][270][271][272][273][274][275] Figure 7 demonstrates modification of carbon-based nanomaterials with CS and then, conjugation of folic acid as ligand on nanocarriers to mediate their internalization in cervical cancer cells via endocytosis, resulting in a significant increase in accumulation of DOX in tumor cells. 253,276,277 however, there is no experiment pertaining to the use of CS-based nanocarriers for DOX delivery in treatment of cancer patients that exploits the afore mentioned benefits of CS.…”

Section: Metal Nanoparticle Modificationmentioning

confidence: 99%

Chitosan‐based nanoscale systems for doxorubicin delivery: Exploring biomedical application in cancer therapy

Ashrafizadeh

Hushmandi

Mirzaei

et al. 2022

Bioengineering & Transla Med

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Green chemistry has been a growing multidisciplinary field in recent years showing great promise in biomedical applications, especially for cancer therapy. Chitosan (CS) is an abundant biopolymer derived from chitin and is present in insects and fungi. This polysaccharide has favorable characteristics, including biocompatibility, biodegradability, and ease of modification by enzymes and chemicals. CS‐based nanoparticles (CS‐NPs) have shown potential in the treatment of cancer and other diseases, affording targeted delivery and overcoming drug resistance. The current review emphasizes on the application of CS‐NPs for the delivery of a chemotherapeutic agent, doxorubicin (DOX), in cancer therapy as they promote internalization of DOX in cancer cells and prevent the activity of P‐glycoprotein (P‐gp) to reverse drug resistance. These nanoarchitectures can provide co‐delivery of DOX with antitumor agents such as curcumin and cisplatin to induce synergistic cancer therapy. Furthermore, co‐loading of DOX with siRNA, shRNA, and miRNA can suppress tumor progression and provide chemosensitivity. Various nanostructures, including lipid‐, carbon‐, polymeric‐ and metal‐based nanoparticles, are modifiable with CS for DOX delivery, while functionalization of CS‐NPs with ligands such as hyaluronic acid promotes selectivity toward tumor cells and prevents DOX resistance. The CS‐NPs demonstrate high encapsulation efficiency and due to protonation of amine groups of CS, pH‐sensitive release of DOX can occur. Furthermore, redox‐ and light‐responsive CS‐NPs have been prepared for DOX delivery in cancer treatment. Leveraging these characteristics and in view of the biocompatibility of CS‐NPs, we expect to soon see significant progress towards clinical translation.

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Preparation and characterization of 3D human glioblastoma spheroids using an N-octanoyl glycol chitosan hydrogel

Cited by 6 publications

References 36 publications

Preparation of Assemblable Chondral and Subchondral Bone Microtissues for Osteochondral Tissue Engineering

Preparation of Assemblable Chondral and Subchondral Bone Microtissues for Osteochondral Tissue Engineering

Comparison of 3D and 2D cultures for evaluating the effect of combination therapy with Erlotinib and Imatinib on Glioblastoma multiforme

Chitosan‐based nanoscale systems for doxorubicin delivery: Exploring biomedical application in cancer therapy

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