Preparation and characteristics of electrospinning PTH‐Fc/PLCL/SF membranes for bioengineering applications

Abstract

Alveolar bone loss is always a big challenge for oral implant placement. Guided bone regeneration (GBR) is one of the solutions to fix this problem. Among all the factors, the barrier membrane plays a key role in this technology. At present, lack of the osteoinductivity remains the biggest weakness for clinical application. Focusing on this, we tried to fabricate an osteoinductive three-dimensional (3D) nanofiber membrane with drug releasing system by introducing parathyroid hormone (PTH)-Fc into the poly(lactic acid-co-caprolactone) PLCL/SF solution for electrospinning. The characteristics were determined by scanning electron microscopy, X-ray diffraction, contact angle measurement, and mechanical strength. According to the optimum parameters tested, we proceed to the in vitro experiments. Drug release and matrix degradation profiles of electrospinning fibers were investigated. Cell morphology was observed and cell proliferation was analyzed after bone marrow mesenchymal stem cells (BMMSCs) were seeded. The results showed favorable physical and chemical properties and good biocompatibility of these 3D nanofibers. The effect on the BMMSCs suggested the drug releasing promoted osteogenic differentiation of cells stably. This study exhibited a promising method of GBR oeteoinductive membrane fabrication.The bone reconstruction will be benefited from the osteogenic differentiation positive drug releasing system. K E Y W O R D S electrospinning, membrane, guided bone regeneration, PTH-Fc

Electrospun metformin-loaded polycaprolactone/chitosan nanofibrous membranes as promoting guided bone regeneration membranes: Preparation and characterization of fibers, drug release, and osteogenic activity in vitro

“…Generally, the biphasic pattern of initial burst release and subsequent steady release was a typical release profile observed in ordinary electrospinning groups. Most studies about drugs or osteogenic factors-loaded electrospun membranes 58–61 reported good osteogenic properties of their developed membranes. While the release rate and total release have an influence on the osteogenic effect.…”

Parathyroid Hormone 1 Receptor Signaling in Dental Mesenchymal Stem Cells: Basic and Clinical Implications

“…Several new methods have been developed to provide more convenient and effective ways to apply PTH or PTHrP as well as to reduce side effects. Among the various delivery methods proposed are an implantable and biodegradable pulsatile device ( Dang et al, 2017 ), bio-membrane fabrication ( Yin et al, 2020 ), and a nanoemulsion system ( Altaani et al, 2020 ). Most of the current research has not progressed beyond the pre-clinical phase, thus detailed guidance for dosage, frequency, duration, and clinical indication of PTH or PTHrP administration in orofacial region is still required.…”

Preparation and performance of random- and oriented-fiber membranes with core–shell structures via coaxial electrospinning

“…Fibrous scaffolds prepared by electrospinning are capable of mimicking the complex structure of a natural extracellular matrix ( Hasan et al, 2014 ; Ingavle and Kent, 2014 ; Kishan and Hernandez, 2017 ; Zong et al, 2018 ; Eilenberg et al, 2020 ), and they have large specific surface areas and a high-porosity microstructures that facilitate cell and nutrient infiltration ( Yin et al, 2020a ; Guo et al, 2022 ). The method of coaxial electrospinning can prepare functional fiber membranes ( Ezhilarasu et al, 2019 ; Johnson et al, 2021 ; Zhang et al, 2021 ).…”