Preparation and Characterisation of Nobiletin-Loaded Nanostructured Lipid Carriers

Abstract

The objective of this manuscript was to investigate and optimise the potential of nanostructured lipid carriers (NLCs) as a carrier system for nobiletin (NOB), which was prepared by high-pressure homogenisation method. Additionally, this study was focused on the application of NOB-loaded NLC (NOB-NLC) in functional food. Response surface method with a three-level Box-Behnken design was validated through analysis of variance, and the robustness of the design was confirmed through the correspondence between the values measured in the experiments and the predicted ones. Properties of the prepared NOB-NLC, such asaverage, polydispersity, entrapment efficiency, zeta potential, morphology, and crystallinity, were investigated. NOB-NLC exhibited a spherical shape with a diameter of 112.27 ± 5.33 nm, zeta potential of −35.1 ± 2.94 mV, a polydispersity index of 0.251 ± 0.058, and an EE of 81.06% ± 6.02%. Results from X-ray diffraction and differential scanning calorimetry of NOB-NLC reviewed that the NOB crystal might be converted to an amorphous state. Fourier transform infrared spectroscopic analysis demonstrated that chemical interaction was absent between the compound and lipid mixture in NOB-NLC.

Enhanced delivery of melatonin loaded nanostructured lipid carriers duringin vitrofertilization: NLC formulation, optimization and IVF efficacy

“…The particle size of NLCs drops as the liquid lipid concentration increases (Fig. 1b), which is consistent with the results reported by Huang et al 38 It has been shown that the viscosity of melted GMS alone is higher than the viscosity of GMS and liquid lipid matrix. Therefore, this matrix of lipids can reduce viscosity and surface tension in the internal phase of NLC, which forms smaller nanoparticles.…”

“…The "Adeq Precision" factor represents the signal-to-noise ratio of the model, which is deemed acceptable for values higher than 4. 38 The values of "Adeq Precision" for the entrapment efficiency and particle size responses are 13.602 and 13.544, respectively, which demonstrates the sufficiency of models for both responses. 3.1.2.…”

“…The solvent layer attached to the particle improves the results of PSA. 35,38 According to the AFM image, peaks have approximately the same height, which indicates the uniform distribution of particles in the optimal sample. These results are consistent with those reported by Huang and et al 38 In their study, NLC nanoparticles also had a spherical and uniform structure.…”

Enhanced bactericidal effect of ceftriaxone drug encapsulated in nanostructured lipid carrier against gram-negative Escherichia coli bacteria: drug formulation, optimization, and cell culture study

“…TEM and DLS results are relatively identical, and the difference between TEM and DLS analysis is due to the measurement method. The DLS analysis measures the hydrodynamic diameter of particles in the solution, indicating a larger diameter for particles [22,23]. Moreover, the polydispersity index (PDI) of 0.131 reveals the uniform distribution of particles, which is aligned with AFM images (Fig.…”

Development of protocol for screening of formulation attributes and the assessment of common quality problems in oleuropein loaded nanostructured lipid carriers

“…Drug unloaded NLC was taken as a negative control and Oleuropein drug loaded NLC as a positive control to minimize the difference between spectra due to baseline shifts. IR spectrums were compared to investigate the interaction between drug and NLC (Wei Huang et al, 2017).…”