Preparation and characterisation of calcium phosphate–hyaluronic acid nanocomposite bone cement

Abstract

In this study, calcium phosphate cements (CPCs) were prepared by using hyaluronic acid (HA) solutions with two different molecular weights. The physical, physicochemical and structural properties of these cements were characterised and compared with those of conventional CPC as control group. Results revealed that the long setting time and the low compressive strength of the CPC could be improved drastically by using HA in the cement composition, in a molecular weight dependent manner. The HA polymer has also a promising effect on cement injectability, which is more considerable in high molecular weight HA. The X-ray diffraction patterns of set cements showed that, in both control group and HA containing cements, primary reactant components were completely converted to nanostructured apatite after soaking in simulated body fluid. This study suggests that calcium phosphate nanocomposite cement obtained by HA solution can be successfully used as injectable bone filler material.

Probing the microporosity and 3D spatial distribution of calcium phosphate cement/hydrogel biomaterials using FIB/SEM at cryogenic temperatures

“…It has been reported that, in the presence of HA, the formation of such particles can be related to the precipitation of an apatitic phase. 31 These particles are present more in CDHA/Si-HA than in CDHA/gelatin or the control cement, which might be the result of the enhanced precipitation of the hydroxyapatite related to the presence of HA carboxylic groups. 44 These groups have been reported to favor the nucleation of apatite crystals.…”

“…Structural and chemical analyses were performed on calcium deficient hydroxyapatite (CDHA) as the control group and on CDHA mixed with two different hydrogels: gelatin that is a physical crosslink hydrogel and silanized hyaluronic acid (Si-HA) that is a covalent one. 27 Apart from the fact that CPC/ gelatin and CPC/hyaluronic acid composites are among the most widely studied biomaterials in bone regeneration, [28][29][30][31][32][33] they exhibit a highly hydrated fragile nature, which makes them typical samples of interest that could benefit from optimized protocols in cryo-FIB/SEM.…”

Design Strategies and Biomimetic Approaches for Calcium Phosphate Scaffolds in Bone Tissue Regeneration

“…Incorporation of sodium alginate caused a general decrease in setting behavior with different power/liquid ratios and increase in compressive and tensile strength of the hardened biomaterial [ 199 ]. The employment of other types of biopolymers, such as hyaluronic acid [ 200 , 201 ] and chitosan [ 202 ] revealed promising effects on cement injectability, making CPCs a viable example of bone filler for tissue engineering.…”

Hyaluronic-Acid-Based Organic-Inorganic Composites for Biomedical Applications

“…The properties of CPC-HYH were influenced by chemical interactions of the carboxylic groups of HYH and Ca ions on the surface of the CPC phase [ 88 ]. Investigations revealed the influence of the HYH phase on the CPC setting time and injection behavior [ 88 , 89 ].…”