Preparation and characterisation of an<sup>57</sup>Fe enriched haemoglobin spike material for species-specific isotope dilution mass spectrometry

Brauckmann, Christine; Frank, Claudia; Schulze, Dietmar; Kaiser, Patricia; Stosch, Rainer; Swart, Claudia

doi:10.1039/c6ja00028b

Cited by 9 publications

(6 citation statements)

References 29 publications

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“…Therefore, absolute methods using species-specific isotope dilution methods have been developed to identify and also validate sample preparation approaches for these target species, and they give highly accurate and precise data [ 16 ]. This concept has only recently been extended to specific metalloproteins such as superoxide dismutase [ 17 ] and haemoglobin [ 18 ]. The accuracy and precision comes, however, at a price, since the methods are not cheap and they are complex and very slow.…”

Section: Bespoke Methods For Targeted Speciation Studies (Status Quo mentioning

confidence: 99%

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

2017

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This article is aimed at researchers interested in organic molecules which contain a heteroatom but who have never considered using inductively coupled plasma mass spectrometry (ICPMS) or who have used ICPMS for years and developed numerous methods for analysis of target elemental species. We try to illustrate (1) that ICPMS has been very useful for speciation analysis of metal(loid) target species and that there is now a trend to replace the costly detector with cheaper detection systems for routine target analysis, and (2) that ICPMS has been used and will be used even more in the future for non-targeted analysis of elements which are not normally associated with ICPMS analysis, such as non-metals such as sulfur, phosphorus, chlorine and fluorine. Graphical AbstractStarting with HPLC-ICPMS for non-targeted analysis of heteroatom containing molecules, once target molecule is identified alternative detectors can be used for routine measurements

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Section: Bespoke Methods For Targeted Speciation Studies (Status Quo mentioning

confidence: 99%

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

2017

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“…Due to the low ionization efficiency of heteroatoms (such as phosphorus and sulfur which are covalently bonded to the protein's primary structure) and its susceptibility to severe spectral line interference, 24,25 it is difficult to measure the heteroatoms by ICP-MS directly, and the measurement of heteroatoms is also interfered by polyatomic ions. 26,27 In fact, the use of the collision reaction cells and high-resolution ICP-MS instrumentation to remove polyatomic interferences may hinder the applicability of ICP-MS quantification in biological applications, usually requiring low limits of detection (LOD). 28,29 In this sense, the introduction of tandem configurations into ICP-MS instrumentation (ICP-QQQ) provided an efficient way for the elimination of polyatomic interferences and so for highly sensitive detection of non-metallic heteroatoms.…”

Section: Absolute Quantification Of Metalloproteins By Using Icp-ms Naturally Present Elements (P S Se and Metals)mentioning

confidence: 99%

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Feng¹

2021

At.Spectrosc.

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The emerging field of metrometallomics refers to the qualitative and quantitative measurement of metallic analytes in the metrological sciences. Inductively coupled plasma mass spectrometry (ICP-MS) has the merits of multi-elemental quantification with very low detection limits. It has been applied for the quantification of peptides and proteins by measuring the concentration of certain elements, which is a very important applications area in metrometallomics. This review showcases the application of ICP-MS on the quantification of metalloproteins through the quantification of protein-bound metal/metalloid elements, intrinsic metal/metalloid elements, or labeled metal/metalloid elements. The results of study also suggest that metrometallomics can be a useful tool in the development of certified reference materials of metalloproteins.

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“…This approach can be used in quantitative proteomics measuring isotope ratios in elements either naturally present in the peptides/proteins, or tagged to them, usually with reagent molecules. Hence, absolute quantification has been reported for either amino acids, peptides, and even full‐length proteins …”

Section: Stable‐isotope Labeled Standardization Approachesmentioning

confidence: 99%

Standardization approaches in absolute quantitative proteomics with mass spectrometry

Calderón-Celis

Encinar

Sanz‐Medel

2017

Mass Spectrometry Reviews

122

110

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Mass spectrometry-based approaches have enabled important breakthroughs in quantitative proteomics in the last decades. This development is reflected in the better quantitative assessment of protein levels as well as to understand post-translational modifications and protein complexes and networks. Nowadays, the focus of quantitative proteomics shifted from the relative determination of proteins (ie, differential expression between two or more cellular states) to absolute quantity determination, required for a more-thorough characterization of biological models and comprehension of the proteome dynamism, as well as for the search and validation of novel protein biomarkers. However, the physico-chemical environment of the analyte species affects strongly the ionization efficiency in most mass spectrometry (MS) types, which thereby require the use of specially designed standardization approaches to provide absolute quantifications. Most common of such approaches nowadays include (i) the use of stable isotope-labeled peptide standards, isotopologues to the target proteotypic peptides expected after tryptic digestion of the target protein; (ii) use of stable isotope-labeled protein standards to compensate for sample preparation, sample loss, and proteolysis steps; (iii) isobaric reagents, which after fragmentation in the MS/MS analysis provide a final detectable mass shift, can be used to tag both analyte and standard samples; (iv) label-free approaches in which the absolute quantitative data are not obtained through the use of any kind of labeling, but from computational normalization of the raw data and adequate standards; (v) elemental mass spectrometry-based workflows able to provide directly absolute quantification of peptides/proteins that contain an ICP-detectable element. A critical insight from the Analytical Chemistry perspective of the different standardization approaches and their combinations used so far for absolute quantitative MS-based (molecular and elemental) proteomics is provided in this review.

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Preparation and characterisation of an⁵⁷Fe enriched haemoglobin spike material for species-specific isotope dilution mass spectrometry

Abstract: A species-specific ID-ICP-MS method for intact haemoglobin was developed applying an 57Fe enriched haemoglobin spike, which was prepared and characterised carefully.

Cited by 9 publications

References 29 publications

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Standardization approaches in absolute quantitative proteomics with mass spectrometry

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Preparation and characterisation of an57Fe enriched haemoglobin spike material for species-specific isotope dilution mass spectrometry

Abstract: A species-specific ID-ICP-MS method for intact haemoglobin was developed applying an 57Fe enriched haemoglobin spike, which was prepared and characterised carefully.

Cited by 9 publications

References 29 publications

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

Inductively Coupled Plasma Mass Spectrometry For Metrometallomics: The Study Of Quantitative Metalloproteins

Standardization approaches in absolute quantitative proteomics with mass spectrometry

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Product

Resources

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Preparation and characterisation of an⁵⁷Fe enriched haemoglobin spike material for species-specific isotope dilution mass spectrometry