Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D

Abney, Erika R.; Hunter, I.R.; Parkhouse, R. M. E.

doi:10.1038/259404a0

Cited by 60 publications

(30 citation statements)

References 17 publications

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“…Additionally, in this experiment, pretreatment with a putative anti-mouse 6-chain antiserum [23] had no effect on responses to either an in vitro or an in viyo boost. This result has been confirmed in one other experiment with these clones, in which the anti-Fab (though exceptionally not the anti-p) blocked successfully in parallel groups (not shown).…”

Section: Repeat Experimentsmentioning

confidence: 80%

“…The subclass-specific antisera were raised against mouse myeloma proteins, and absorbed with insoluble myeloma proteins of different classes and subclasses, and their specificity was confirmed by radioimmunoassay. The preparation and characterization of the specific rabbit anti-mouse &-chain antiserum has also been described in detail [23].…”

Section: Anti-immunoglobulin Antiseramentioning

confidence: 99%

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Immunoglobulin M receptors on memory cells of immunoglobulin G antibody‐forming cell clones

Abney¹,

Keeler²,

Parkhouse³

et al. 1976

Eur J Immunol

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The memory cells of two antibody-forming cell clones had receptors of the IgM class, even though the clones had been producing IgG1 or IgG2a anti-2,4-dinitrophenyl antibodies for 9-15 months previously (on exposure to antigen). Thus a phenotypic switch in heavy chain constant region evidently occurred after re-exposure of these memory cells to antigen. To show that, we first removed the clonal cells' surface immunoglobins by "capping" and "stripping", with class- or subclass-specific antisera. Then, to assay their remaining receptor activity, the cells were incubated with antigen in vitro, washed and transferred (together with carrier primed cells) to irradiated recipients, and their antibody responses to this in vitro boost were assayed by iselectric focusing. Pretreatment with anti-mu serum, as well as with anti-Fab(kappa), prevented the responses of the IgG1 and IgG2a clones to an in vitro boost, while anti-gamma1 and anti-gamma2a antisera had no effect. An antiserum to the putative mouse IgD also had no effect. The anti-mu serum failed to react with the IgG1 and IgG2A clonal serum antibodies in the test tube. Some other contaminating clones were suppressed completely only by the anti-Fab serum. This result strongly suggests that switching in class commitment may occur during the differentiation of memory cells to antibody producers, and may therefore be antigen-dependent. It also implies that some apparently naive cells with surface IgM may, in reality, be B memory cells.

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Section: Repeat Experimentsmentioning

confidence: 80%

Section: Anti-immunoglobulin Antiseramentioning

confidence: 99%

Immunoglobulin M receptors on memory cells of immunoglobulin G antibody‐forming cell clones

Abney¹,

Keeler²,

Parkhouse³

et al. 1976

Eur J Immunol

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“…The antiserum had been produced against a purified fraction of SC sIgD by a modification of the technique of Abney et al [35], as described previously [15]. Details of the membrane extract preparation, immunization and absorption procedures and of the assays for specificity have been reported elsewhere [15].…”

Section: Antisera Used For Immunofluorescence Stainingmentioning

confidence: 99%

The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes V. IgD‐bearing cells in immunological tolerance

Zan‐Bar

Barzilay

1982

Eur J Immunol

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The role of IgD (delta) in the induction of tolerance in murine B lymphocytes was explored in the in vivo adoptive antibody response to bovine serum albumin (BSA). Delta+ or delta- B cells purified on the fluorescence-activated cell sorter were injected, with or without purified T cells, into lethally irradiated intermediate hosts, which were rendered tolerant by an injection of deaggregated BSA one day after the cell transfer. Spleen cells from the intermediate hosts were treated with anti-Thy-1.2 antiserum and complement mixed with normal T cells and were transferred to final hosts. The ability of the B cells of the final hosts to respond to the tolerogen BSA and to dinitrophenylated human gamma globulin antigen was examined. This approach enabled examination of the function of the B cell subpopulations in an environment free of tolerogen and of induced T suppressor cells. The results revealed that in the presence of T cells the delta+ subpopulation was highly resistant to tolerance induction, whereas the delta- subpopulation was highly susceptible to tolerance induction. However, there was no difference in susceptibility to tolerance induction between the two subpopulations when tolerance was induced in the absence of T cells.

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“…Table 1 gives the distiibntion ol cell surface IgM and IgD on eaeh of these spleen cell populations. Onr restilts show a higher percentage of eells with both IgM and IgD than was found by Abney et al (1976) in adult animals. This is probalily due to differences in sensitivity caused by different optical systems and antisera, together with tlie considerable heterogeneity of brightness of individual ceUs.…”

Section: Resultsmentioning

confidence: 85%

DISTRIBUTION OF SURFACE IgM AND IgD ON SINGLE CELLS FROM LYMPHOCYTE SUBPOPULATIONS

Layton

Howard

1978

Aust J Exp Biol Med

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Summary. The surface immunoglobulin hea^y chains on individual spleen cells fractionated l)y velocity sedimentation were studied using lUmrescent antisera. In adult mice, cells bcarins both ii and 6 chains were found in all fractions. While there was an increase in the proportion of cells bearing \i only in the medium to large cell fraetioii.s, the majority of cells bearing )i only were small lymphocytes. Results obtained using 3-week-old mice were basically similar, but showed both a marked decrease in small |i + 6+ celLs and a marked iricrea.se in small ji + ft-cells when compared wilh adnlt animals.

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Preparation and characterisation of an antiserum to the mouse candidate for immunoglobulin D

Cited by 60 publications

References 17 publications

Immunoglobulin M receptors on memory cells of immunoglobulin G antibody‐forming cell clones

Immunoglobulin M receptors on memory cells of immunoglobulin G antibody‐forming cell clones

The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes V. IgD‐bearing cells in immunological tolerance

DISTRIBUTION OF SURFACE IgM AND IgD ON SINGLE CELLS FROM LYMPHOCYTE SUBPOPULATIONS

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