Preparation and certification of solutions of perdeuterated polycyclic aromatic compounds intended for use as surrogate internal standards

Quilliam, Michael A.; Hardstaff, W. R.; Anacleto, Joseph F.; LeBlanc, Mark; Stergiopoulos, Victonia; Dick, Karla L.; Bowser, Michael T.; Curtis, Jonathan M.; Embree, D. J.; Sim, P. G.; Boyd, Robert K.

doi:10.1007/bf00323170

Cited by 26 publications

(12 citation statements)

References 20 publications

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“…Standard solutions of perdeuterated compounds (DPAC-2 containing 7 PAHs and DPAC-1 containing 21 perdeuterated PAHs), developed 30 by the Certified Reference Materials Program of the National Research Council (NRC) Canada, were used as test solution and internal standard for quantification experiments, respectively. A DPAC-1 solution was spiked at five different levels into the NIST SRM 1597 coal tar mixture, in ratios of 1:1, 1:2, 1:3, 1:4, and 1:4.75 (v/v).…”

Section: Methodsmentioning

confidence: 99%

Isomeric identification and quantification of polycyclic aromatic hydrocarbons in environmental samples by liquid chromatography tandem mass spectrometry using a high pressure quadrupole collision cell

Mansoori

1998

Rapid Commun. Mass Spectrom.

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Section: Methodsmentioning

confidence: 99%

Isomeric identification and quantification of polycyclic aromatic hydrocarbons in environmental samples by liquid chromatography tandem mass spectrometry using a high pressure quadrupole collision cell

Mansoori

1998

Rapid Commun. Mass Spectrom.

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“…An HP GC (Hewlett-Packard, Palo Alto, CA, USA) equipped with a split/splitless injector was used. The PAHs were quantified relative to perdeuterated PAHs (Quilliam et al 1994;Baumard and Budzinski 1997). The response factors of the different compounds were measured by injecting a standard reference material [SRM 2260 (24 aromatic hydrocarbons in toluene) (NIST, Gaithersburg, MD, USA) solution spiked with the same solution containing the perdeuterated PAHs as the one used for spiking the fish liver].…”

Section: B[a]p Analysismentioning

confidence: 99%

Acute effects of benzo[a]pyrene on liver phase I and II enzymes, and DNA damage on sea bream Sparus aurata

Bannı¹,

Bouraoui²,

Ghedira³

et al. 2008

Fish Physiol Biochem

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In the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons benzo[a]pyrene (B[a]P) were investigated in the liver of Sparus aurata (sea bream). Sexually immature gilthead sea bream were treated by intraperitoneal injection of B[a]P (20 mg kg(-1)) for 6, 12, 24, and 48 h. B[a]P accumulation was quantified in sea bream liver by mean of gas phase chromatography (GPC-MS) after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity, as a phase I biotransformation parameter; (2) liver glutathione S-transferase (GST) activity as a phase II conjugation enzyme. DNA damage was assessed over time using the single-cell gel electrophoresis comet assay. B[a]P bioaccumulation in the liver resulted in a biphasic curve with an increasing uptake up to 5.55 +/- 0.67 microg g(-1) dry weight after only 6 h exposure and 4.67 +/- 0.68 microg g(-1) dry weight after 48 h exposure. EROD activity showed a nonsymmetrical bell-shaped kinetic with a maximum at 24 h and lower but significant activities at 12 and 48 h with respect to control animals. Hepatic GST activities were only significant after 48 h exposure. Comet assay showed an increase in liver cells DNA damage with a maximum after 48 h exposure reaching up to 12.17 % DNA in the tail.

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“…The principle of surrogate standard quantification is well known and described elsewhere [22,23]. The standards are added to the sample matrix and are taken through the entire analytical procedure -extraction, clean-up, chromatography.…”

Section: Resultsmentioning

confidence: 99%

Multi-residue analysis of polycyclic aromatic hydrocarbons, polychlorobiphenyls, and organochlorine pesticides in marine sediments

et al. 2001

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A multi-residue analysis procedure using microwave-assisted extraction and pre-purification has been developed for the combined analysis of polycyclic aromatic hydrocarbons (PAH), polychlorobiphenyls (PCB), and organochlorine pesticides (OCP) in marine sediments. This procedure has been validated with certified marine sediment. Several surrogate standards have been employed and the use of octachloronaphthalene (OCN) as a surrogate standard for organochlorine determination in this matrix is discussed. The recoveries of all compounds were high (>70%) and the relative standard deviations are of the same order as the certified values. Different analytical problems are discussed, including DDT degradation in gas chromatography and laboratory PCB background levels. Quantification problems encountered for two pesticides (cis-chlordane and trans-nonachlor) were attributed to PAH interference in the GC-ECD chromatogram.

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Preparation and certification of solutions of perdeuterated polycyclic aromatic compounds intended for use as surrogate internal standards

Cited by 26 publications

References 20 publications

Isomeric identification and quantification of polycyclic aromatic hydrocarbons in environmental samples by liquid chromatography tandem mass spectrometry using a high pressure quadrupole collision cell

Isomeric identification and quantification of polycyclic aromatic hydrocarbons in environmental samples by liquid chromatography tandem mass spectrometry using a high pressure quadrupole collision cell

Acute effects of benzo[a]pyrene on liver phase I and II enzymes, and DNA damage on sea bream Sparus aurata

Multi-residue analysis of polycyclic aromatic hydrocarbons, polychlorobiphenyls, and organochlorine pesticides in marine sediments

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