Preparation and biological activities of chitosan oligosaccharides

Benchamas, Gunsriwiang; Huang, Gangliang; Huang, Shiyu; Huang, Hualiang

doi:10.1016/j.tifs.2020.11.027

Cited by 135 publications

(77 citation statements)

References 62 publications

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“…Nevertheless, chitosan is insoluble in water and soluble only in some inorganic and organic acids, such as acetic, hydrochloric, lactic, and citric acids, among which acetic acid is the most commonly used (Nascimento et al, 2020). Chitooligosaccharides (COSs), are degradation products of chitosan consisting of depolymerized derivatives of chitosan with a degree of polymerization (DP) ranging from 2 to 10 ( Benchamas et al, 2021). COSs are nontoxic, water-soluble, and biocompatible and have been widely used as antitumor (Jiang et al, 2020), drug-delivery (Wang et al, 2021a), antimicrobial (Silva et al, 2021), antifungal (Zhou et al, 2020), immune-activating (Zhang et al, 2021), and food preservation agents (Dutta et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Cui

Yang

et al. 2021

Front. Microbiol.

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Chitosanase plays a vital role in bioactive chitooligosaccharide preparation. Here, we characterized and prepared a potential GH46 family chitosanase from Bacillus atrophaeus BSS. The purified recombinant enzyme Csn-SH showed a molecular weight of 27.0 kDa. Csn-SH displayed maximal activity toward chitosan at pH 5.0 and 45°C. Thin-layer chromatography and electrospray ionization–mass spectrometry indicated that Csn-SH mainly hydrolyzed chitosan into (GlcN)2, (GlcN)3, and (GlcN)4 with an endo-type cleavage pattern. Molecular docking analysis demonstrated that Csn-SH cleaved the glycoside bonds between subsites −2 and + 1 of (GlcN)6. Importantly, the chitosan hydrolysis rate of Csn-SH reached 80.57% within 40 min, which could reduce time and water consumption. The hydrolysates prepared with Csn-SH exhibited a good antifungal activity against Magnaporthe oryzae and Colletotrichum higginsianum. The above results suggested that Csn-SH could be used to produce active chitooligosaccharides efficiently that are biocontrol agents applicable for safe and sustainable agricultural production.

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Section: Introductionmentioning

confidence: 99%

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Cui

Yang

et al. 2021

Front. Microbiol.

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“…42 An enzyme, chitosanase which fights fungal infections has been isolated from shells of shrimps, crabs and lobsters. 43 An extract named paolin I from clams, oysters and abalone has antibacterial properties including against the streptococcal infections. 44 A related agent paolin II has anti-cancerous and anti-viral properties particularly against herpes virus.…”

Section: Novel Compounds From Other Marine Creaturesmentioning

confidence: 99%

Marine natural products: the new generation of pharmacotherapeutics

Goel

Mahendru

Saini

2021

Int J Basic Clin Pharmacol

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The biomedical potential of the sea has gone largely unexplored so far, despite the fact that it covers three quarters of the planet surface and the fact that life on Earth originated from the sea. However, with the arrival of the professional deep sea divers, the marine researchers have gained access to all sorts of marine creatures like sponges, corals, sea urchins, sea squirts, hydroids, sea anemones, fishes and mollusks as well as to varied types of sea plants including algae and the other micro-organisms embedded in the sea bed. The biomedical scientists are exploiting these all to extract marine natural products (MNPs) having pharmacological properties that may one day cure long list of illnesses varying from bacterial infections to cancer, Alzheimer's and AIDS and was the focus of this review article.

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“…They also have a remarkable potential to be applied in pharmacological and biomedical fields. Compared with chitosan and chitin, COSs are equipped with reactive functional groups, such as hydroxyl groups at C3 and C6, while the amino and N -glucosamine units are linked by glycosides [ 11 , 12 ]. These properties are crucial for COSs’ natural biological effects and applicable utilization.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Caco-2 Cell Transport Assay of Chito-Oligosaccharides Nano-Liposomes Based on Layer-by-Layer Coated

et al. 2021

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Chito-oligosaccharides (COSs) were encapsulated by the film-ultrasonic method into three nano-liposomes, which were uncoated liposomes (COSs-Lip), chitosan-coated liposomes (CH-COSs-Lip), and sodium alginate (SA)/chitosan (CH)-coated liposomes (SA/CH-COSs-Lip). The physicochemical and structural properties, as well as the stability and digestive characteristics, of all three nano-liposomes were assessed in the current study. Thereafter, the characteristics of intestinal absorption and transport of nano-liposomes were investigated by the Caco-2 cell monolayer. All nano-liposomes showed a smaller-sized distribution with a higher encapsulation efficiency. The ζ-potential, Z-average diameter (Dz), and polydispersity index (PDI) demonstrated that the stability of the SA/CH-COSs-Lip had much better stability than COSs-Lip and CH-COSs-Lip. In addition, the transport of the nano-liposomes via the Caco-2 cell monolayer indicated a higher transmembrane transport capacity. In summary, the chitosan and sodium alginate could serve as potential delivery systems for COSs to fortify functional foods and medicines.

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Preparation and biological activities of chitosan oligosaccharides

Cited by 135 publications

References 62 publications

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

Marine natural products: the new generation of pharmacotherapeutics

Characterization and Caco-2 Cell Transport Assay of Chito-Oligosaccharides Nano-Liposomes Based on Layer-by-Layer Coated

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